OsO2 as the Contrast‐Generating Chemical Species of Osmium‐Stained Biological Tissues in Electron Microscopy
Ruiyu Li,
Gregg Wildenberg,
Kevin Boergens
et al.
Abstract:Electron imaging of biological samples stained with heavy metals has enabled visualization of subcellular structures critical in chemical‐, structural‐, and neuro‐biology. In particular, osmium tetroxide OsO4 has been widely adopted for selective lipid imaging. Despite the ubiquity of its use, the osmium speciation in lipid membranes and the process for contrast generation in electron microscopy (EM) have continued to be open questions, limiting efforts to improve staining protocols and therefore high‐resoluti… Show more
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