OsNAC6, a member of the NAC gene family, is induced by various stresses in rice

Ohnishi, Takayuki; Sugahara, Seishi; Yamada, Toshiko; Kikuchi, Kazuhiro; Yoshiba, Yoshu; Hirano, Hideki; Tsutsumi, Nobuhiro

doi:10.1266/ggs.80.135

Cited by 162 publications

(117 citation statements)

References 26 publications

Supporting

Mentioning

108

Contrasting

Order By: Relevance

“…The SNAC1-overexpressing rice plants also showed improved salt tolerance, further emphasizing the usefulness of this gene in stress tolerance improvement (Hu et al 2006). Another stress-responsive NAC gene OsNAC6, which is a member of ATAF subfamily (Kikuchi et al 2000;Ooka et al 2003), has been reported for its induction by abiotic stresses and jasmonic acid treatment (Ohnishi et al 2005), and overexpression of this gene in rice resulted in enhanced tolerance to dehydration stresses (Nakashima et al 2007).…”

mentioning

confidence: 92%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

View full text Add to dashboard Cite

Plants respond to adverse environment by initiating a series of signaling processes including activation of transcription factors that can regulate expression of arrays of genes for stress response and adaptation. NAC (NAM, ATAF, and CUC) is a plant specific transcription factor family with diverse roles in development and stress regulation. In this report, a stress-responsive NAC gene (SNAC2) isolated from upland rice IRA109 (Oryza sativa L. ssp japonica) was characterized for its role in stress tolerance. SNAC2 was proven to have transactivation and DNA-binding activities in yeast and the SNAC2-GFP fusion protein was localized in the rice nuclei. Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment. The SNAC2 gene was over-expressed in japonica rice Zhonghua 11 to test the effect on improving stress tolerance. More than 50% of the transgenic plants remained vigorous when all WT plants died after severe cold stress (4-8°C for 5 days). The transgenic plants had higher cell membrane stability than wild type during the cold stress. The transgenic rice had significantly higher germination and growth rate than WT under high salinity conditions. Over-expression of SNAC2 can also improve the tolerance to PEG treatment. In addition, the SNAC2-overexpressing plants showed significantly increased sensitivity to ABA. DNA chip profiling analysis of transgenic plants revealed many up-regulated genes related to stress response and adaptation such as peroxidase, ornithine aminotransferase, heavy metal-associated protein, sodium/hydrogen exchanger, heat shock protein, GDSL-like lipase, and phenylalanine ammonia lyase. Interestingly, none of the up-regulated genes in the SNAC2-overexpressing plants matched the genes up-regulated in the transgenic plants over-expressing other stress responsive NAC genes reported previously. These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.

show abstract

mentioning

confidence: 92%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

View full text Add to dashboard Cite

show abstract

“…For example, transcript accumulation was observed for RD26 in response to cold stress [44], but this was not found with ATAF1 (data not shown). Recently, OsNAC6, a rice ortholog of ATAF1, was reported to be a positive contributor to disease resistance and salinity tolerance [29,30]. This finding may indicate the functional discrepancies of NAC genes between monocotyledon and dicotyledon plants.…”

Section: Functional Overlap Between Ataf1 and Other Stressregulatory mentioning

confidence: 99%

“…Additionally, the rice gene OsNAC6 was found to be an abiotic and biotic stress-related gene. OsNAC6-overexpression plants were tolerant to dehydration/high-salinity stresses and showed slightly increased tolerance to blast disease [29,30].…”

Section: Introductionmentioning

confidence: 99%

Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses

et al. 2009

View full text Add to dashboard Cite

NAC family genes encode plant-specific transcription factors involved in diverse biological processes. In this study, the Arabidopsis NAC gene ATAF1 was found to be induced by drought, high-salinity, abscisic acid (ABA), methyl jasmonate, mechanical wounding, and Botrytis cinerea infection. Significant induction of ATAF1 was found in an ABA-deficient mutant aba2 subjected to drought or high salinity, revealing an ABA-independent mechanism of expression. Arabidopsis ATAF1-overexpression lines displayed many altered phenotypes, including dwarfism and short primary roots. Furthermore, in vivo experiments indicate that ATAF1 is a bona fide regulator modulating plant responses to many abiotic stresses and necrotrophic-pathogen infection. Overexpression of ATAF1 in Arabidopsis increased plant sensitivity to ABA, salt, and oxidative stresses. Especially, ATAF1 overexpression plants, but not mutant lines, showed remarkably enhanced plant tolerance to drought. Additionally, ATAF1 overexpression enhanced plant susceptibility to the necrotrophic pathogen B. cinerea, but did not alter disease symptoms caused by avirulent or virulent strains of P. syringae pv tomato DC3000. Transgenic plants overexpressing ATAF1 were hypersensitive to oxidative stress, suggesting that reactive oxygen intermediates may be related to ATAF1-mediated signaling in response to both pathogen and abiotic stresses.

show abstract

“…In addition to ATAF1, 2, a number of NAC proteins were shown to play roles in biotic and abiotic responses. OsNAC6, an ATAF homolog in rice, is rapidly and strongly induced by cold, salt, drought, abscisic acid, jasmonic acid and wounding, indicating its role in both biotic and abiotic responses (Ohnishi et al, 2005;Nakashima et al, 2007). Recently, OsNAC5, like OsNAC6, was characterized as a transcriptional activator that enhances stress tolerance by upregulating the expression of stress-inducible rice genes (Sperotto et al, 2009;Takasaki et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

A structural view of the conserved domain of rice stress-responsive NAC1

et al. 2011

View full text Add to dashboard Cite

The importance of NAC (named as NAM, ATAF1, 2, and CUC2) proteins in plant development, transcription regulation and regulatory pathways involving proteinprotein interactions has been increasingly recognized. We report here the high resolution crystal structure of SNAC1 (stress-responsive NAC) NAC domain at 2.5 Å. Although the structure of the SNAC1 NAC domain shares a structural similarity with the reported structure of the ANAC NAC1 domain, some key features, especially relating to two loop regions which potentially take the responsibility for DNA-binding, distinguish the SNAC1 NAC domain from other reported NAC structures. Moreover, the dimerization of the SNAC1 NAC domain is demonstrated by both soluble and crystalline conditions, suggesting this dimeric state should be conserved in this type of NAC family. Additionally, we discuss the possible NAC-DNA binding model according to the structure and reported biological evidences.

show abstract

OsNAC6, a member of the NAC gene family, is induced by various stresses in rice

Cited by 162 publications

References 26 publications

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses

A structural view of the conserved domain of rice stress-responsive NAC1

Contact Info

Product

Resources

About