OsMYB103 is essential for tapetum degradation in rice

Lei, Ting; Zhang, Lisha; Feng, Ping; Liu, Yang; Yin, Wuzhong; Shang, Lei; He, Guanghua; Wang, Nan

doi:10.1007/s00122-021-04007-6

Cited by 18 publications

(31 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several investigations have revealed key regulators of both tapetum development and exine formation in rice. These include four bHLH TFs (UDT1, TDR, TIP2/OsbHLH142, and EAT1/DTD) (Fu et al., 2014; Ji et al., 2013; Jung et al., 2005; Ko et al., 2014; Li et al., 2006; Niu, Liang, et al., 2013), three MYB TFs (GAMYB, OsMS188/OsMYB80/OsMYB103, and OsTDF1) (Aya et al., 2009; Cai et al., 2015; Han et al., 2021; Lei et al., 2022; Liu et al., 2010; Pan et al., 2020), two PHD‐finger TFs (PTC1/OsMS1 and TIP3) (Li et al., 2011; Yang, Liu, et al., 2019; Yang, Sun, et al., 2019), and an AHL domain‐containing TF (PTC2) (Uzair et al., 2019). In this study, we present the cytological observations, expression analysis, and biochemical findings on the critical regulatory role of AMD1, a novel transcription activator involved in rice tapetum degradation and exine formation.…”

Section: Discussionmentioning

confidence: 99%

“…The degradation of anther wall layers including the tapetum is delayed in dmd1 (Ren et al., 2020). The mutant of the MYB TF‐encoding gene MALE STERILITY188 ( OsMS188 )/ OsMYB80 / OsMYB103 shows abnormal tapetum development (Han et al., 2021; Lei et al., 2022; Pan et al., 2020). Another two MYB TFs, rice DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (OsTDF1) and GIBBERELLIN MYB (GAMYB), were also reported to modulate tapetum development (Aya et al., 2009; Cai et al., 2015; Liu et al., 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, TDR also interacts with DMD1 and TIP3 (Ren et al., 2020; Yang, Sun, et al., 2019). The transcription of OsMS188 / OsMYB80 / OsMYB103 is directly regulated by TDR, and its protein also interacts with TDR (Han et al., 2021; Lei et al., 2022). Simultaneously, OsMS188/OsMYB80/OsMYB103 can bind directly to the promoters of EAT1 / DTD and PTC1 / OsMS1 to affect their expression (Lei et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

“…The transcription of OsMS188 / OsMYB80 / OsMYB103 is directly regulated by TDR, and its protein also interacts with TDR (Han et al., 2021; Lei et al., 2022). Simultaneously, OsMS188/OsMYB80/OsMYB103 can bind directly to the promoters of EAT1 / DTD and PTC1 / OsMS1 to affect their expression (Lei et al., 2022). In addition to forming a complex with TIP2/OsbHLH142 (Yang, Liu, et al., 2019), PTC1/OsMS1 also associates with TDR to regulate the expression of EAT1 / DTD (Wu et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Grass‐specific ABERRANT MICROSPORE DEVELOPMENT 1 is required for maintaining pollen fertility in rice

et al. 2022

View full text Add to dashboard Cite

Pollen development includes a series of biological events that require precise gene regulation. Although several transcription factors (TFs) have been shown to play roles in maintaining pollen fertility, the major regulatory networks underlying tapetum development and pollen wall formation are largely unknown. Herein, we report that ABERRANT MICROSPORE DEVELOPMENT1 (AMD1), a protein annotated previously as unknown protein, is required for tapetum development and pollen exine patterning in rice (Oryza sativa L.). AMD1 encodes a grass-specific protein exhibiting transactivation activity in the nucleus and is spatiotemporally expressed in the tapetum and microspores during pollen development. Further biochemical assays indicate that AMD1 directly activates the transcription of DEFECTIVE POLLEN WALL (DPW) and POLYKETIDE SYNTHASE2 (OsPKS2), which are both implicated in sporopollenin biosynthesis during exine formation. Additionally, AMD1 directly interacts with TAPETUM DEGENERATION RETARDATION (TDR), a key TF involved in the regulation of tapetum degradation and exine formation. Taken together, we demonstrate that AMD1 is an important regulatory component involved in the TDR-mediated regulatory pathway to regulate sporopollenin biosynthesis, tapetum degradation, and exine formation for pollen development.Our work provides insights into the regulatory network of rice sexual reproduction and a useful target for genetic engineering of new male-sterile lines for hybrid rice breeding.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Grass‐specific ABERRANT MICROSPORE DEVELOPMENT 1 is required for maintaining pollen fertility in rice

et al. 2022

View full text Add to dashboard Cite

show abstract

“…After the morphogenesis of the anther, the microspores are enclosed by the tapetum, which could secrete sporopollenin and provide essential nutrients for microspore development by secreting vesicles and self-degradation [ 36 , 37 ]. Studies have shown that the abnormal PCD of tapetum will affect pollen exine pattern formation and microspore development, thereby leading to pollen abortion and MS [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (Brassica juncea var. tumida Tsen et Lee)

Shen

Wang

Xia

et al. 2022

IJMS

View full text Add to dashboard Cite

The development of flower and pollen is a complex biological process that involves multiple metabolic pathways in plants. In revealing novel insights into flower and pollen development underlying male sterility (MS), we conducted an integrated profiling of gene and protein activities in developing buds in cytoplasmic male sterile (CMS) mutants of mustard (Brassica juncea). Using RNA-Seq and label-free quantitative proteomics, 11,832 transcripts and 1780 protein species were identified with significant differential abundance between the male sterile line 09-05A and its maintainer line 09-05B at the tetrad stage and bi-nucleate stage of B. juncea. A large number of differentially expressed genes (DEGs) and differentially abundant proteins (DAPs) involved in carbohydrate and energy metabolism, including starch and sucrose metabolism, tricarboxylic acid (TCA) cycle, glycolysis, and oxidoreductase activity pathways, were significantly downregulated in 09-05A buds. The low expression of these DEGs or functional loss of DAPs, which can lead to an insufficient supply of critical substrates and ATP, could be associated with flower development, pollen development, and changes in fertility in B. juncea. Therefore, this study provided transcriptomic and proteomic information of pollen abortion for B. juncea and a basis for further research on the molecular regulatory mechanism of MS in plants.

show abstract

The vesicle trafficking gene, OsRab7, is critical for pollen development and male fertility in cytoplasmic male-sterility rice

Ying,

Tang,

Yang

et al. 2024

Gene

View full text Add to dashboard Cite

OsMYB103 is essential for tapetum degradation in rice

Cited by 18 publications

References 45 publications

Grass‐specific ABERRANT MICROSPORE DEVELOPMENT 1 is required for maintaining pollen fertility in rice

Grass‐specific ABERRANT MICROSPORE DEVELOPMENT 1 is required for maintaining pollen fertility in rice

Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (Brassica juncea var. tumida Tsen et Lee)

The vesicle trafficking gene, OsRab7, is critical for pollen development and male fertility in cytoplasmic male-sterility rice

Contact Info

Product

Resources

About