Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters

Zemančíková, Jana; Dušková, Michala; Elicharová, Hana; Papoušková, Klára; Sychrová, Hana

doi:10.1093/femsle/fny020

Cited by 4 publications

(6 citation statements)

References 18 publications

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“…As described by Hohmann (2002) and Zemancíková et al (2018), glycerol proton symporters (Stl1 and Stl2) are important for the osmotic stress resistance of yeasts (e.g., Dekkera bruxellensis and Saccharomyces cerevisiae) through glycerol synthesis and its intracellular accumulation. Most prokaryotic and eukaryotic organisms tolerate hypersaline conditions using an "organic solute-in strategy" through the accumulation of "compatible solutes" (e.g., polyols in fungi) to maintain their intracellular Na + concentrations below the levels that would be toxic for the cells (Hohmann, 2002).…”

Section: Differential Expression Analysismentioning

confidence: 99%

Improving Azo Dye Decolorization Performance and Halotolerance of Pichia occidentalis A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis

Wang

Ning

et al. 2020

Front. Microbiol.

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A halotolerant yeast, Pichia occidentalis A2, was recently isolated that can decolorize various azo dyes. The azo dye decolorization performance of this strain was characterized, including the degradation pathway and detoxification effects of this yeast. Additionally, the effect of static magnetic field (SMF) on this decolorization process was investigated. Activities of key enzymes were analyzed to estimate the change of metabolic activity. Furthermore, possible mechanisms were analyzed through detecting differentially expressed genes between yeast A2 in the absence and presence of SMF. The results indicated that yeast A2 displayed the optimal decolorization performance when the concentrations (in g/L) of glucose, (NH 4) 2 SO 4 , yeast extract, and NaCl were 4.0, 1.0, 0.1, and ≤30.0, respectively. Meanwhile, the optimal rotation speed, temperature, and pH were 160 rpm, 30 • C, and 5.0, respectively. Acid Red B was decolorized and detoxified by yeast A2 through successive steps, including cleavage of the naphthalene-amidine bond, reductive deamination, oxidative deamination/desulfurization, open-loop of hydroxy-substituted naphthalene, and tricarboxylic acid cycle. The dye decolorization efficiency and halotolerance of yeast A2 were enhanced by 206.3 mT SMF. The activities of manganese peroxidase, and laccase were elevated 1.37-and 1.16-fold by 206.3 mT SMF, but lignin peroxidase activity showed little change. It was suggested from the transcriptome sequence that the enhanced halotolerance might be related to the upregulated genes encoding the enzymes or functional proteins related to intracellular synthesis and accumulation of glycerol.

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Section: Differential Expression Analysismentioning

confidence: 99%

Improving Azo Dye Decolorization Performance and Halotolerance of Pichia occidentalis A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis

Wang

Ning

et al. 2020

Front. Microbiol.

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“…Analysis of its secondary structure revealed that it is a typical member of the STL family with 12 trans‐membrane segments and conserved motifs typical of this family (Zemancikova et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…(Zemancikova et al . ) was followed. For measuring the intracellular pH, the procedure using cells expressing ratiometric pHluorin described in Ref.…”

Section: Methodsmentioning

confidence: 99%

Stl1 transporter mediating the uptake of glycerol is not a weak point of Saccharomyces kudriavzevii's low osmotolerance

Zemančíková

Papoušková

Pérez‐Torrado

et al. 2018

Lett Appl Microbiol

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Significance and Impact of the Study: An increasing demand for food products with benefits for human health turns the attention to less-exploited nonconventional yeasts with interesting traits not found in Saccharomyces cerevisiae. Among them, Saccharomyces kudriavzevii has good potential for aroma-compound production, fermentations and other biotechnological applications, but it is less adapted to stressful industrial conditions. This report studied S. kudriavzevii relative osmosensitivity and its capacity for active glycerol uptake. The results obtained (on the activity and physiological function of S. kudriavzevii glycerol transporter) may contribute to a further engineering of this species aiming to improve its osmotolerance. AbstractSaccharomyces kudriavzevii is a nonconventional and rather osmosensitive yeast with a high potential of use in fermentation processes. To elucidate the basis of its relative osmosensitivity, the role of the STL1 gene encoding a putative glycerol uptake system was studied. Under higher osmotic pressure, the addition of a low amount of glycerol to the growth medium improved the growth of S. kudriavzevii and the expression of the STL1 gene was highly induced. Deletion of this gene decreased the strain's ability to grow in the presence of higher concentrations of salts and other solutes. Moreover, the mutant had a disturbed homeostasis of intracellular pH. Expression of the SkSTL1 gene in Saccharomyces cerevisiae complemented the osmosensitivity of the S. cerevisiae hog1D stl1D mutant, and the gene's tagging with GFP localized its product to the plasma membrane. Altogether, a deficiency in glycerol uptake did not seem to be the reason for S. kudriavzevii's low osmotolerance; its Stl1 transporter properly contributes to the regulation of intracellular pH and is crucial to its survival of osmotic stress.Letters in Applied Microbiology ISSN 0266-8254

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“…Bootstrap values below 500 are not shown. The S. cerevisiae glycerol transporter Stl1p and its B. bruxellensis ortholog (Zemancíková et al 2018) were used as outgroups. GenBank protein accession numbers for S. cerevisiae transporters are displayed.…”

Section: Resultsmentioning

confidence: 99%

Identification and characterisation of two high-affinity glucose transporters from the spoilage yeastBrettanomyces bruxellensis

Tiukova

Møller-Hansen

Belew

et al. 2019

FEMS Microbiology Letters

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The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.

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Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters

Cited by 4 publications

References 18 publications

Improving Azo Dye Decolorization Performance and Halotolerance of Pichia occidentalis A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis

Improving Azo Dye Decolorization Performance and Halotolerance of Pichia occidentalis A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis

Stl1 transporter mediating the uptake of glycerol is not a weak point of Saccharomyces kudriavzevii's low osmotolerance

Identification and characterisation of two high-affinity glucose transporters from the spoilage yeastBrettanomyces bruxellensis

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