Osmotin gene expression is controlled by elicitor synergism

Chang, Pi-Fang Linda; Cheah, Kheng T.; Narasimhan, Meena L.; Hasegawa, Paul M.; Bressan, Ray A.

doi:10.1034/j.1399-3054.1995.950417.x

Cited by 4 publications

(4 citation statements)

References 21 publications

(38 reference statements)

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“…For instance, the induction of tobacco genes for vacuolar PR-2 and PR-3 proteins after infection by Erwinia carotovora was similar in nahG -expressing plants and wild-type plants (Vidal et al, 1997). Pathogen-responsive tobacco genes for vacuolar PR-2, PR-3, and PR-5 proteins are known to be induced much more efficiently by the external application of ethylene than SA (Brederode et al, 1991; Xu et al, 1994;Beffa et al, 1995), and elicitor-induced induction of a vacuolar PR-5 gene in tobacco could be blocked by norbornadiene, which is a blocker of ethylene action (Chang et al, 1995). In Arabidopsis, we have recently identified a gene ( PDF1.2 ) encoding an antifungal peptide belonging to the family of plant defensins (Penninckx et al, 1996; see Broekaert et al, 1995, for a review on plant defensins).…”

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confidence: 99%

Concomitant Activation of Jasmonate and Ethylene Response Pathways Is Required for Induction of a Plant Defensin Gene in Arabidopsis

et al. 1998

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Activation of the plant defensin gene PDF1.2 in Arabidopsis by pathogens has been shown previously to be blocked in the ethylene response mutant ein2-1 and the jasmonate response mutant coi1-1. In this work, we have further investigated the interactions between the ethylene and jasmonate signal pathways for the induction of this defense response. Inoculation of wild-type Arabidopsis plants with the fungus Alternaria brassicicola led to a marked increase in production of jasmonic acid, and this response was not blocked in the ein2-1 mutant. Likewise, A. brassicicola infection caused stimulated emission of ethylene both in wild-type plants and in coi1-1 mutants. However, treatment of either ein2-1 or coi1-1 mutants with methyl jasmonate or ethylene did not induce PDF1.2 , as it did in wild-type plants. We conclude from these experiments that both the ethylene and jasmonate signaling pathways need to be triggered concomitantly, and not sequentially, to activate PDF1.2 upon pathogen infection. In support of this idea, we observed a marked synergy between ethylene and methyl jasmonate for the induction of PDF1.2 in plants grown under sterile conditions. In contrast to the clear interdependence of the ethylene and jasmonate pathways for pathogen-induced activation of PDF1.2 , functional ethylene and jasmonate signaling pathways are not required for growth responses induced by jasmonate and ethylene, respectively. INTRODUCTIONHigher plants induce various defense responses when they are attacked by microbial pathogens, such as fungi, bacteria, or viruses. These defense responses include suicide of the attacked host cell (the so-called hypersensitive response); the production of antimicrobial secondary metabolites (called phytoalexins); the production of pathogenesisrelated (PR) proteins, of which many exert antimicrobial properties; and the production and oxidative cross-linking of cell wall polymers. The efficacy of these defense responses often determines whether plants are susceptible to infection by a pathogen.Elicitors secreted by or released from microbial invaders are the primary signal for induction of plant defense responses (Ebel and Cosio, 1994). Each pathogen produces a particular mixture of elicitors, which are sometimes accompanied by suppressors, and these molecules interact with receptors on the host cells that further translate the primary signal into particular events in the plasma membrane, the cytosol, and/or the nucleus (Shirasu et al., 1996). Induction of some defense genes requires the generation of secondary endogenous signal molecules (stress hormones) by the challenged cells in the infection site. The secondary signal molecules in turn set in motion signal transduction cascades in receiving cells, eventually leading to activation of pathogen-responsive genes. Secondary signal molecules thus serve to amplify and spread the response of the host after initial recognition of the pathogen. Several secondary signal molecules whose synthesis is increased in response to elicitor recognition and that ar...

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confidence: 99%

Concomitant Activation of Jasmonate and Ethylene Response Pathways Is Required for Induction of a Plant Defensin Gene in Arabidopsis

et al. 1998

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“…Osmotins, whose expression is related to drought resistance, salt tolerance, and plant disease resistance, is a kind of newly synthesized or increased protein when plant faces osmosis stress [ 21 ]. Besides, the accumulation of osmotins may be a kind of elementary immune reaction generated by plants for original immune response, and osmotins may even act as the dehydration storage protein which also possesses the antifungal activity [ 22 ]. From above, the expression increase of two proteins, HSP (No.15) and osmotin (No.13), may protect cellular structure and play a role in the repair of cellular dysfunction during sugarcane- S. scitamineum interaction.…”

Section: Discussionmentioning

confidence: 99%

Differential Protein Expression in Sugarcane during Sugarcane-Sporisorium scitamineumInteraction Revealed by 2-DE and MALDI-TOF-TOF/MS

Que

Lin

et al. 2011

Comparative and Functional Genomics

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To understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant proteins that respond to the pathogen attack. Two sugarcane varieties, NCo376 and Ya71-374, were used in this study. By applying 2-dimensional electrophoresis (2-DE), the protein expression profile of sugarcane after inoculating with Sporisorium scitamineum was analyzed. In total, 23 differentially expressed proteins were identified by MALDI-TOF-TOF/MS. Bioinformatics analysis revealed that the functions of these 20 differential proteins were associated with such functions as photosynthesis, signal transduction, and disease resistance, while the function of the remaining three proteins was not determined. From above, we can assume that the protein regulatory network during the interaction between sugarcane and S. scitamineum is complicated. This represents the first proteomic investigation focused on highlighting the alterations of the protein expression profile in sugarcane exposed to S. scitamineum, and it provides reference information on sugarcane response to S. scitamineum stress at the protein level.

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“…Procedures used for RNA extraction, northem hybridization and immunoblot detection and probes for the de-symptoms such as yellowing cotyledons and brown tection of osmotin mRNA and protein have been de-roots in the absence of spores (Fig. 2, data for MeJA scribed (Chang et al 1995). PR-lb mRNA was detected alone not shown).…”

Section: Rna and Protein Analysesmentioning

confidence: 99%

“…Only the protein-type elicitors have been (Nelson et al 1992). Homozygous seeds were germifound to induce resistance in plants without directly in-nated as described (Chang et al 1995). Five-day-old hibiting fungal germination (Ricci et al 1989, Ebel and seedlings were used for all treatment experiments.…”

Section: Introductionmentioning

confidence: 99%

Induction of pathogen resistance and pathogenesis-related genes in tobacco by a heat-stable Trichoderma mycelial extract and plant signal messengers

Chang¹,

Xu²,

Narasimhan³

et al. 1997

Physiol Plant

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1997. Induction of pathogen resistance and pathogenesis-related genes in tobacco by a heat-stable Trichoderma mycelial extract and plant signal messengers. -Physiol. Plant. 100: 341-352.Heat-stable mycelial extracts of the nonpathogenic fungus Trichoderma longibrachiatum induced resistance in tobacco seedlings {Nicotiana tabacum L. cv. Wisconsin 38) to the pathogen Phytophthora parasitica var. nicotianae (race 0), which did not involve a hypersensitive response. Resistance could not be induced with mycelial extract prepared in the same manner from P. parasitica. The nonpathogenic mycelial extract induced expression of PR-lb and osmotin (PR-5) genes to a higher level than did mycelial extract from the pathogenic fungus. The tissue-specific pattem of PR gene induction by the nonpathogenic mycelial extract was different from that of the pathogenic mycelial extract and was consistent with the ability of the former to cause disease resistance. The expression pattems of these two PR genes and the accumulations of their encoded proteins also were affected by salicylic acid (SA), methyl jasmonate (MeJA), ethylene (E) and combinations of these plant signal messengers. However, only combined SA and MeJA treatment mimicked the pattem of PR gene mRNA and protein accumulation induced by the nonpathogenic mycelial extract. E inhibitors blocked both mycelial extract-induced and SA/MeJA-induced PR gene expression, and the cis pattem of responsiveness on the osmotin promoter was the same for the mycelial extract, SA, E, or E/MeJA. Seedlings treated with P. parasitica spores in the presence of SA/MeJA were protected from pathogen colonization. However, these seedlings exhibited symptoms of cell death (disease symptoms) both in the absence and presence of P. parasitica spores, in contrast to seedlings treated with nonpathogenic mycelial extract, which remained healthy. These results suggest that the signal transduction pathways for elicitation of defense responses by exogenously applied heat-stable nonpathogenic mycelial extract and SA/MeJA overlap at the point of PR protein induction but are not identical.

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Osmotin gene expression is controlled by elicitor synergism

Cited by 4 publications

References 21 publications

Concomitant Activation of Jasmonate and Ethylene Response Pathways Is Required for Induction of a Plant Defensin Gene in Arabidopsis

Concomitant Activation of Jasmonate and Ethylene Response Pathways Is Required for Induction of a Plant Defensin Gene in Arabidopsis

Differential Protein Expression in Sugarcane during Sugarcane-Sporisorium scitamineumInteraction Revealed by 2-DE and MALDI-TOF-TOF/MS

Induction of pathogen resistance and pathogenesis-related genes in tobacco by a heat-stable Trichoderma mycelial extract and plant signal messengers

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