Osmotic Stress Leads to Significant Changes in Rice Root Metabolic Profiles between Tolerant and Sensitive Genotypes

Matsunami, Toshinori; Toyofuku, Kyoko; Kimura, Natsumi; Ogawa, Atsushi

doi:10.3390/plants9111503

Cited by 13 publications

(8 citation statements)

References 51 publications

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“… Zhang P et al (2016) observed that salt-tolerant wild soybean accumulate higher contents of citric acid under salt stress. The fumaric acid content of tolerant varieties increases significantly under abiotic stress ( Matsunami et al, 2020 ). In the present study, 2, 3-dihydroxybenzoic acid and phenylacetate were significantly upregulated in KR under Al stress, whereas citraconic acid and fumaric acid were significantly downregulated in MR ( Supplementary Tables S4, 5 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics and metabolomics reveal tolerance new mechanism of rice roots to Al stress

Wang

Su²,

Cui³

et al. 2023

Front. Genet.

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The prevalence of soluble aluminum (Al) ions is one of the major limitations to crop production worldwide on acid soils. Therefore, understanding the Al tolerance mechanism of rice and applying Al tolerance functional genes in sensitive plants can significantly improve Al stress resistance. In this study, transcriptomics and metabolomics analyses were performed to reveal the mechanism of Al tolerance differences between two rice landraces (Al-tolerant genotype Shibanzhan (KR) and Al-sensitive genotype Hekedanuo (MR) with different Al tolerance. The results showed that DEG related to phenylpropanoid biosynthesis was highly enriched in KR and MR after Al stress, indicating that phenylpropanoid biosynthesis may be closely related to Al tolerance. E1.11.1.7 (peroxidase) was the most significant enzyme of phenylpropanoid biosynthesis in KR and MR under Al stress and is regulated by multiple genes. We further identified that two candidate genes Os02g0770800 and Os06g0521900 may be involved in the regulation of Al tolerance in rice. Our results not only reveal the resistance mechanism of rice to Al stress to some extent, but also provide a useful reference for the molecular mechanism of different effects of Al poisoning on plants.

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Section: Discussionmentioning

confidence: 99%

Transcriptomics and metabolomics reveal tolerance new mechanism of rice roots to Al stress

Wang

Su²,

Cui³

et al. 2023

Front. Genet.

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“…Previous studies have shown that plants commonly accumulate amino acids under abiotic stresses, the majority of which are preferentially allocated to tolerance-related processes, such as osmotic adjustment, signal transduction and mitochondrial respiration instead of gene translation, to increase survival rate [ 65 – 69 ]. In comparison to stress sensitive rice, both the concentration and number of amino acids have been observed to be significantly enhanced in stress-tolerant rice under various stress conditions [ 70 , 71 ]. We speculate that the greater accumulation of amino acids in stress-tolerant plants might satisfy the demand of amino acids for not only the tolerance-related processes but also gene translation, which is supported by our observations of more DTGs and greater RPF occupancy on transcripts in SR86 than in NB exposed to salt stress (Figs.…”

Section: Discussionmentioning

confidence: 99%

Comparative ribosome profiling reveals distinct translational landscapes of salt-sensitive and -tolerant rice

et al. 2021

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Background Soil salinization represents a serious threat to global rice production. Although significant research has been conducted to understand salt stress at the genomic, transcriptomic and proteomic levels, few studies have focused on the translatomic responses to this stress. Recent studies have suggested that transcriptional and translational responses to salt stress can often operate independently. Results We sequenced RNA and ribosome-protected fragments (RPFs) from the salt-sensitive rice (O. sativa L.) cultivar ‘Nipponbare’ (NB) and the salt-tolerant cultivar ‘Sea Rice 86’ (SR86) under normal and salt stress conditions. A large discordance between salt-induced transcriptomic and translatomic alterations was found in both cultivars, with more translationally regulated genes being observed in SR86 in comparison to NB. A biased ribosome occupancy, wherein RPF depth gradually increased from the 5′ ends to the 3′ ends of coding regions, was revealed in NB and SR86. This pattern was strengthened by salt stress, particularly in SR86. On the contrary, the strength of ribosome stalling was accelerated in salt-stressed NB but decreased in SR86. Conclusions This study revealed that translational reprogramming represents an important layer of salt stress responses in rice, and the salt-tolerant cultivar SR86 adopts a more flexible translationally adaptive strategy to cope with salt stress compared to the salt susceptible cultivar NB. The differences in translational dynamics between NB and SR86 may derive from their differing levels of ribosome stalling under salt stress.

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“…Capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS) analysis was used to investigate the metabolic profiles of two rice ( Oryza sativa L.) cultivars resistant (IR 58) and sensitive (Basilanon) to osmotic stress [ 101 ]. IR 58 showed an increased accumulation of stress-induced metabolites involved in sugar metabolism (sucrose 6′-phosphate, glucose 1-phosphate), polyamine, and phenylpropanoid metabolisms (spermine, spermidine, gamma-aminobutyric acid (GABA)), and glutathione metabolism (glutathione, cysteine, cadaverine).…”

Section: Metabolomics For the Elucidation Of Abiotic Stress In Plants...mentioning

confidence: 99%

Applications of Metabolomics for the Elucidation of Abiotic Stress Tolerance in Plants: A Special Focus on Osmotic Stress and Heavy Metal Toxicity

Mashabela

Masamba

Kappo

2023

Plants

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Plants undergo metabolic perturbations under various abiotic stress conditions; due to their sessile nature, the metabolic network of plants requires continuous reconfigurations in response to environmental stimuli to maintain homeostasis and combat stress. The comprehensive analysis of these metabolic features will thus give an overview of plant metabolic responses and strategies applied to mitigate the deleterious effects of stress conditions at a biochemical level. In recent years, the adoption of metabolomics studies has gained significant attention due to the growing technological advances in analytical biochemistry (plant metabolomics). The complexity of the plant biochemical landscape requires sophisticated, advanced analytical methods. As such, technological advancements in the field of metabolomics have been realized, aided much by the development and refinement of separatory techniques, including liquid and gas chromatography (LC and GC), often hyphenated to state-of-the-art detection instruments such as mass spectrometry (MS) or nuclear resonance magnetic (NMR) spectroscopy. Significant advances and developments in these techniques are briefly highlighted in this review. The enormous progress made thus far also comes with the dawn of the Internet of Things (IoT) and technology housed in machine learning (ML)-based computational tools for data acquisition, mining, and analysis in the 4IR era allowing for broader metabolic coverage and biological interpretation of the cellular status of plants under varying environmental conditions. Thus, scientists can paint a holistic and comprehensive roadmap and predictive models for metabolite-guided crop improvement. The current review outlines the application of metabolomics and related technological advances in elucidating plant responses to abiotic stress, mainly focusing on heavy metal toxicity and subsequent osmotic stress tolerance.

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Osmotic Stress Leads to Significant Changes in Rice Root Metabolic Profiles between Tolerant and Sensitive Genotypes

Cited by 13 publications

References 51 publications

Transcriptomics and metabolomics reveal tolerance new mechanism of rice roots to Al stress

Transcriptomics and metabolomics reveal tolerance new mechanism of rice roots to Al stress

Comparative ribosome profiling reveals distinct translational landscapes of salt-sensitive and -tolerant rice

Applications of Metabolomics for the Elucidation of Abiotic Stress Tolerance in Plants: A Special Focus on Osmotic Stress and Heavy Metal Toxicity

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