Osmotic stress-induced somatic embryo maturation of coffee Coffea arabica L., shoot and root apical meristems development and robustness

Valencia-Lozano, Eliana; Ibarra, Jorge E.; Herrera-Ubaldo, Humberto; Folter, Stefan de; Cabrera-Ponce, José Luis

doi:10.1038/s41598-021-88834-z

Cited by 13 publications

(5 citation statements)

References 59 publications

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“…Accordingly, in our experiments, somatic embryos derived from edited PEMs were induced to maturation on medium with elevated content of gelrite, in the presence of 2-iP. Under such environmental conditions, water content decreases within the SE [ 89 ]. Hence, to determine potential genes involved in embryo development and maturation, we additionally used 15-days old embryos (growing on G9-2iP medium) for RNA-seq analysis.…”

Section: Discussionmentioning

confidence: 99%

Editing of SlWRKY29 by CRISPR-activation promotes somatic embryogenesis in Solanum lycopersicum cv. Micro-Tom

Valencia-Lozano,

Cabrera-Ponce,

Barraza

et al. 2024

PLoS ONE

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At present, the development of plants with improved traits like superior quality, high yield, or stress resistance, are highly desirable in agriculture. Accelerated crop improvement, however, must capitalize on revolutionary new plant breeding technologies, like genetically modified and gene-edited crops, to heighten food crop traits. Genome editing still faces ineffective methods for the transformation and regeneration of different plant species and must surpass the genotype dependency of the transformation process. Tomato is considered an alternative plant model system to rice and Arabidopsis, and a model organism for fleshy-fruited plants. Furthermore, tomato cultivars like Micro-Tom are excellent models for tomato research due to its short life cycle, small size, and capacity to grow at high density. Therefore, we developed an indirect somatic embryo protocol from cotyledonary tomato explants and used this to generate epigenetically edited tomato plants for the SlWRKY29 gene via CRISPR-activation (CRISPRa). We found that epigenetic reprogramming for SlWRKY29 establishes a transcriptionally permissive chromatin state, as determined by an enrichment of the H3K4me3 mark. A whole transcriptome analysis of CRISPRa-edited pro-embryogenic masses and mature somatic embryos allowed us to characterize the mechanism driving somatic embryo induction in the edited tomato cv. Micro-Tom. Furthermore, we show that enhanced embryo induction and maturation are influenced by the transcriptional effector employed during CRISPRa, as well as by the medium composition and in vitro environmental conditions such as osmotic components, plant growth regulators, and light intensity.

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Section: Discussionmentioning

confidence: 99%

Editing of SlWRKY29 by CRISPR-activation promotes somatic embryogenesis in Solanum lycopersicum cv. Micro-Tom

Valencia-Lozano,

Cabrera-Ponce,

Barraza

et al. 2024

PLoS ONE

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“…Shoot sections (14 µm) were obtained on a rotary microtome (Reichert-Jung 2040, Leica (Hernalser Hauptstrasse, Vienna); and the root semithin sections (500 nm) with a Leica EM UC7 ultramicrotome Leica (Hernalser Hauptstrasse, Vienna). Tissue sections were stained with a 0.02% toluidine blue solution (HYCEL, Zapopan, Mexico) for 3 min, washed with distilled water for 1 min, and then air dried [86]. Shoot images were taken with a DM6000B microscope (Leica) and root photos were taken with a DMi8 inverted microscope (Leica).…”

Section: Histology Of C Citratus Shoots and Rootsmentioning

confidence: 99%

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

Cárdenas-Aquino,

Camas-Reyes,

Valencia-Lozano

et al. 2023

Plants

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The known activities of cytokinins (CKs) are promoting shoot multiplication, root growth inhibition, and delaying senescence. 6-Benzylaminopurine (BAP) has been the most effective CK to induce shoot proliferation in cereal and grasses. Previously, we reported that in lemongrass (Cymbopogon citratus) micropropagation, BAP 10 µM induces high shoot proliferation, while the natural CK 6-(γ,γ-Dimethylallylamino)purine (2-iP) 10 µM shows less pronounced effects and developed rooting. To understand the molecular mechanisms involved, we perform a protein–protein interaction (PPI) network based on the genes of Brachypodium distachyon involved in shoot proliferation/repression, cell cycle, stem cell maintenance, auxin response factors, and CK signaling to analyze the molecular mechanisms in BAP versus 2-iP plants. A different pattern of gene expression was observed between BAP- versus 2-iP-treated plants. In shoots derived from BAP, we found upregulated genes that have already been demonstrated to be involved in de novo shoot proliferation development in several plant species; CK receptors (AHK3, ARR1), stem cell maintenance (STM, REV and CLV3), cell cycle regulation (CDKA-CYCD3 complex), as well as the auxin response factor (ARF5) and CK metabolism (CKX1). In contrast, in the 2-iP culture medium, there was an upregulation of genes involved in shoot repression (BRC1, MAX3), ARR4, a type A-response regulator (RR), and auxin metabolism (SHY2).

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“…While the effects of low osmoticum are not fully understood, it is apparent that water limitation can induce cellular changes favoring the embryogenic and conversion processes. Recently, Valencia-Lozano et al (2021) showed that 9.0 g/L gelrite (−1.47 MPa) enhanced the conversion of Coffea arabica somatic embryos from 39% to 95% [75]. A manipulation of sucrose levels can also influence SE [25].…”

Section: Osmotic Stressmentioning

confidence: 99%

Transduction of Signals during Somatic Embryogenesis

Elhiti

Stasolla

2022

Plants

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Somatic embryogenesis (SE) is an in vitro biological process in which bipolar structures (somatic embryos) can be induced to form from somatic cells and regenerate into whole plants. Acquisition of the embryogenic potential in culture is initiated when some competent cells within the explants respond to inductive signals (mostly plant growth regulators, PRGs), and de-differentiate into embryogenic cells. Such cells, “canalized” into the embryogenic developmental pathway, are able to generate embryos comparable in structure and physiology to their in vivo counterparts. Genomic and transcriptomic studies have identified several pathways governing the initial stages of the embryogenic process. In this review, the authors emphasize the importance of the developmental signals required for the progression of embryo development, starting with the de-differentiation of somatic cells and culminating with tissue patterning during the formation of the embryo body. The action and interaction of PGRs are highlighted, along with the participation of master regulators, mostly transcription factors (TFs), and proteins involved in stress responses and the signal transduction required for the initiation of the embryogenic process.

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Osmotic stress-induced somatic embryo maturation of coffee Coffea arabica L., shoot and root apical meristems development and robustness

Cited by 13 publications

References 59 publications

Editing of SlWRKY29 by CRISPR-activation promotes somatic embryogenesis in Solanum lycopersicum cv. Micro-Tom

Editing of SlWRKY29 by CRISPR-activation promotes somatic embryogenesis in Solanum lycopersicum cv. Micro-Tom

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

Transduction of Signals during Somatic Embryogenesis

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