Osmotic stress‐induced phosphoinositide and inositol phosphate signalling in plants

Munnik, Teun; Vermeer, Joop E. M.

doi:10.1111/j.1365-3040.2009.02097.x

Cited by 222 publications

(192 citation statements)

References 188 publications

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“…These data on salinity effects at the transcript level suggest that the MIB pathway is physiologically important during osmotic stress because it generates high concentrations of the compatible organic osmolyte myo-inositol, which protects cells from salinity-induced damage (Yancey et al, 1982). In addition, myoinositol produced via the MIB pathway represents a substrate for the synthesis of phosphoinositide compounds that are implicated in evolutionarily highly conserved signaling pathways, including osmotic stress signaling (Munnik and Vermeer, 2010).…”

Section: Introductionmentioning

confidence: 99%

Salinity-induced activation of the myo-inositol biosynthesis pathway in tilapia gill epithelium

Sacchi

Villarreal

et al. 2013

Journal of Experimental Biology

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SUMMARYThe myo-inositol biosynthesis (MIB) pathway converts glucose-6-phosphate to the compatible osmolyte myo-inositol that protects cells from osmotic stress. Using proteomics, the enzymes that constitute the MIB pathway, myo-inositol phosphate synthase (MIPS) and inositol monophosphatase 1 (IMPA1), are identified in tilapia (Oreochromis mossambicus) gill epithelium. Targeted, quantitative, label-free proteomics reveals that they are both upregulated during salinity stress. Upregulation is stronger when fish are exposed to severe (34 ppt acute and 90 ppt gradual) relative to moderate (70 ppt gradual) salinity stress. IMPA1 always responds more strongly than MIPS, suggesting that MIPS is more stable during salinity stress. MIPS is N-terminally acetylated and the corresponding peptide increases proportionally to MIPS protein, while non-acetylated N-terminal peptide is not detectable, indicating that MIPS acetylation is constitutive and may serve to stabilize the protein. Hyperosmotic induction of MIPS and IMPA1 is confirmed using western blot and real-time qPCR and is much higher at the mRNA than at the protein level. Two distinct MIPS mRNA variants are expressed in the gill, but one is more strongly regulated by salinity than the other. A single MIPS gene is encoded in the tilapia genome whereas the zebrafish genome lacks MIPS entirely. The genome of euryhaline tilapia contains four IMPA genes, two of which are expressed, but only one is salinity regulated in gill epithelium. The genome of stenohaline zebrafish contains a single IMPA gene. We conclude that the MIB pathway represents a major salinity stress coping mechanism that is regulated at multiple levels in euryhaline fish but absent in stenohaline zebrafish.Supplementary material available online at http://jeb.biologists.org/lookup/suppl

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Section: Introductionmentioning

confidence: 99%

Salinity-induced activation of the myo-inositol biosynthesis pathway in tilapia gill epithelium

Sacchi

Villarreal

et al. 2013

Journal of Experimental Biology

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“…In plants, InsP 3 has been associated with a wide range of cellular functions, such as guard cell physiology (Blatt et al, 1990;Gilroy et al, 1990;Burnette et al, 2003;Han et al, 2003), drought tolerance (Knight et al, 1997;Perera et al, 2008), heat shock responses (Liu et al, 2006), blue light perception (Chen et al, 2008), root gravitropism (Wang et al, 2009;Zhang et al, 2011), response to mechanical wounding (Mosblech et al, 2008), and pollen dormancy (Y. . However, the role of InsP 3 and other inositol phosphates in plant signaling remains controversial as no inositol phosphate receptor has been identified to date (Munnik and Vermeer, 2010;Munnik and Nielsen, 2011;Gillaspy, 2013).…”

Section: Introductionmentioning

confidence: 99%

VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis

et al. 2015

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ORCID IDs: 0000-0002-7823-5489 (D.L.); 0000-0002-4512-9508 (P.J.); 0000-0002-1025-9484 (H.J.J.); 0000-0001-9022-4515 (G.S.)Diphosphorylated inositol polyphosphates, also referred to as inositol pyrophosphates, are important signaling molecules that regulate critical cellular activities in many eukaryotic organisms, such as membrane trafficking, telomere maintenance, ribosome biogenesis, and apoptosis. In mammals and fungi, two distinct classes of inositol phosphate kinases mediate biosynthesis of inositol pyrophosphates: Kcs1/IP6K-and Vip1/PPIP5K-like proteins. Here, we report that PPIP5K homologs are widely distributed in plants and that Arabidopsis thaliana VIH1 and VIH2 are functional PPIP5K enzymes. We show a specific induction of inositol pyrophosphate InsP 8 by jasmonate and demonstrate that steady state and jasmonate-induced pools of InsP 8 in Arabidopsis seedlings depend on VIH2. We identify a role of VIH2 in regulating jasmonate perception and plant defenses against herbivorous insects and necrotrophic fungi. In silico docking experiments and radioligand binding-based reconstitution assays show highaffinity binding of inositol pyrophosphates to the F-box protein COI1-JAZ jasmonate coreceptor complex and suggest that coincidence detection of jasmonate and InsP 8 by COI1-JAZ is a critical component in jasmonate-regulated defenses.

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“…The molecule phosphatidic acid (PA) functions as the plant lipid secondary messenger (reviewed in (Munnik and Vermeer, 2010). In soybean, PA is an upstream activator of the woundinduced MAPK pathway .…”

Section: Phospholipid Signallingmentioning

confidence: 99%

Molecular Responses to Osmotic Stresses in Soybean

Phang¹,

Li²,

Cheng³

et al. 2011

Soybean - Molecular Aspects of Breeding

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Osmotic stress‐induced phosphoinositide and inositol phosphate signalling in plants

Cited by 222 publications

References 188 publications

Salinity-induced activation of the myo-inositol biosynthesis pathway in tilapia gill epithelium

Salinity-induced activation of the myo-inositol biosynthesis pathway in tilapia gill epithelium

VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis

Molecular Responses to Osmotic Stresses in Soybean

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Osmotic stress‐induced phosphoinositide and inositol phosphate signalling in plants

Cited by 222 publications

References 188 publications

Salinity-induced activation of the myo-inositol biosynthesis pathway in tilapia gill epithelium

Salinity-induced activation of the myo-inositol biosynthesis pathway in tilapia gill epithelium

VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis

Molecular Responses to Osmotic Stresses in Soybean

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VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis