Osmotic stress affects water relations, growth, and nitrogen fixation in Phaseolus vulgaris plants

Aydi, Sameh Sassi; Aydi, Samir; González, Esther M.; Abdelly, Chédly

doi:10.1007/s11738-008-0141-y

Cited by 20 publications

(7 citation statements)

References 38 publications

(30 reference statements)

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“…It was able to uptake more water and then grow more when exposed to decreased Ψ o , thus it turned out to be a better drought tolerant cultivar than Cv. 'Coco Blanc' (Sassi et al 2008a). This may be attributed to maintenance of the leaf and nodule water status under stressed conditions (Fig.…”

Section: Water Relationsmentioning

confidence: 99%

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Understanding osmotic stress tolerance in leaves and nodules of two Phaseolus vulgaris cultivars with contrasting drought tolerance

et al. 2010

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Drought and salinity are environmental constraints that affect crop yields worldwide. In nature, both stresses are multifaceted problems that are usually associated with other adverse circumstances which limit plant performance such as water shortage and nutrient deficits. In order to assess common features of both stresses, the effects of mannitol-induced osmotic stress were monitored using two Phaseolus vulgaris cultivars, Cv. 'Flamingo' (tolerant) and Cv. 'Coco Blanc' (sensitive) which differed in their drought and salinity tolerance. Growth, water relations, organic and inorganic compound accumulation and soluble protein contents were measured in leaves and nodules of these N 2 -fixing plants. The aim of the present study was to check whether osmotic stress tolerance is associated with accumulation of some of these compounds either in leaves, nodules or both organs. At the whole-plant level, Cv. 'Flamingo' showed a better maintenance of plant biomass and shoot water status. At the cell level, this was related to a better osmotic adjustment ability both in leaves and nodules and also to a better adjustment of the cell wall elasticity. At the metabolic level, the contrasting accumulation of the different amino acids in nodules of each cultivar suggested that amino acids pathways can be regulated to different degrees under stress conditions. At the metabolic level, it seems that symbiosis in the sink organ (the nodule) plays a crucial role in conferring drought and salinity tolerance in the common bean.

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Section: Water Relationsmentioning

confidence: 99%

“…'Flamingo' (tolerant) and Cv. 'Coco Blanc' (sensitive) was demonstrated (Sassi et al 2008a) These authors showed that nodule carbon metabolism and antioxidant defence was involved in the better stress performance of Cv. 'Flamingo' (Sassi et al 2008b).…”

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Understanding osmotic stress tolerance in leaves and nodules of two Phaseolus vulgaris cultivars with contrasting drought tolerance

et al. 2010

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“…Sodium and potassium were assayed by flame emission photometry (Corning, UK) and chloride concentration determined by coulombic titration against Ag + (Digital chloridometer; Buchler instruments, Kansas, KS, USA), while reduced nitrogen content (mmol per g DW), was determined using the Kjeldahl Method (Bouat and Crouzet 1965). Three plants from each treatment were used for the assessment of nodule nitrogenase activity (EC 1.7.9.92) estimated by the acetylene reduction assay (Hardy et al 1968;Drevon et al 1988;Sassi et al 2008). Ten percent of C 2 H 2 was added to the nodulated-root atmosphere, after incubation, formed ethylene rate was measured using gas chromatograph equipped with a Porapak-T column.…”

Section: Plant Harvest and Measured Parametersmentioning

confidence: 99%

Growth, nitrogen fixation and ion distribution in Medicago truncatula subjected to salt stress

2008

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This study compared the growth, nodulation, N 2 fixation, and ion distribution in three Medicago truncatula lines, in response to salt in nutrient solution. Two local lines (TN8.20 and TN6.18) and a reference line (Jemalong 6) were inoculated with a reference strain Sinorhizobium meliloti 2011, a very tolerant strain to salinity (700 mM NaCl) and grown in a controlled glasshouse with or without 75 mM NaCl. A genotypic variation in tolerance to salt was found: TN6.18 was the most sensitive line whereas TN8.20 was the most tolerant. The relative tolerance of TN8.20 was concomitant with the lowest leaf Na + concentration and the highest nodule biomass production. However, nodule efficiency (amount of nitrogen fixed per g dry weight nodule) decreased in all lines. Results suggest that the tolerance to salt seems to depend on the host plant ability to protect its leaves against an excessive Na + (and Cl − ) accumulation, and its ability to maintain the development of an abundant nodular system, which in turn determines an important rate of nitrogen fixation and allows the plants to conserve their growth potentialities. The loss of the nodular efficiency under salt stress seems to be compensated by a large nodule biomass.

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“…In a previous work, the responses at the whole plant level of four common bean cultivars to osmotic stress were analysed. Results therein showed a better tolerance of Flamingo relative to Coco blanc (Sassi et al 2008). The aim of this study was to analyze the likely mechanisms involved in the tolerance of common bean nodules to long-term osmotic stress.…”

Section: Introductionmentioning

confidence: 99%

Tolerance of common bean to long-term osmotic stress is related to nodule carbon flux and antioxidant defenses: evidence from two cultivars with contrasting tolerance

Sassi¹,

González

Aydi³

et al. 2008

Plant Soil

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The response to the osmotic effects of drought may largely vary not only between species but even cultivars of the same species. Gaining knowledge of the reasons underlying these differential responses can be critical in breeding programs to obtain lines with enhanced performance under drought or salinity. In this work, the responses to osmotic stress of two common bean (Phaseolus vulgaris L.) cultivars with contrasting tolerance (Coco blanc, sensitive, and Flamingo, tolerant) have been assessed by analyzing a full array of nodule parameters (enzyme activities, carbohydrate and organic acids content and antioxidant activity). The aim of this work was to study the likely involvement of carbon flux shortage in the decline of N 2 fixation under osmotic stress. The maintenance of sucrolytic activities, particularly sucrose synthase, to keep an adequate glycolytic flux, together with isocitrate dehydrogenase, to balance C/N interactions, associated with a suitable antioxidant defense may be relevant for osmotic tolerance in common bean nitrogen fixation.

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Osmotic stress affects water relations, growth, and nitrogen fixation in Phaseolus vulgaris plants

Cited by 20 publications

References 38 publications

Understanding osmotic stress tolerance in leaves and nodules of two Phaseolus vulgaris cultivars with contrasting drought tolerance

Understanding osmotic stress tolerance in leaves and nodules of two Phaseolus vulgaris cultivars with contrasting drought tolerance

Growth, nitrogen fixation and ion distribution in Medicago truncatula subjected to salt stress

Tolerance of common bean to long-term osmotic stress is related to nodule carbon flux and antioxidant defenses: evidence from two cultivars with contrasting tolerance

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