Osmotic stress affects functional properties of human melanoma cell lines

Porta, Caterina A. M. La; Ghilardi, Anna; Pasini, Maria Enrica; Laurson, Lasse; Alava, Mikko J.; Zapperi, Stefano; Amar, Martine Ben

doi:10.1140/epjp/i2015-15064-x

Cited by 22 publications

(27 citation statements)

References 26 publications

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“…3) Does the critical lytic tension depend on the strain rate, and thus the strength of the osmotic gradient? Such questions arise beyond this context of vesicle osmoregulation in other important scenarios where the coupling between the dissipation of osmotic energy and cellular compartmentalization has important biological ramifications (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Pulsatile Lipid Vesicles under Osmotic Stress

Chabanon

Liedberg

et al. 2017

Biophysical Journal

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The response of lipid bilayers to osmotic stress is an important part of cellular function. Recent experimental studies showed that when cell-sized giant unilamellar vesicles (GUVs) are exposed to hypotonic media, they respond to the osmotic assault by undergoing a cyclical sequence of swelling and bursting events, coupled to the membrane's compositional degrees of freedom. Here, we establish a fundamental and quantitative understanding of the essential pulsatile behavior of GUVs under hypotonic conditions by advancing a comprehensive theoretical model of vesicle dynamics. The model quantitatively captures the experimentally measured swell-burst parameters for single-component GUVs, and reveals that thermal fluctuations enable rate-dependent pore nucleation, driving the dynamics of the swell-burst cycles. We further extract constitutional scaling relationships between the pulsatile dynamics and GUV properties over multiple timescales. Our findings provide a fundamental framework that has the potential to guide future investigations on the nonequilibrium dynamics of vesicles under osmotic stress.

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Section: Introductionmentioning

confidence: 99%

Pulsatile Lipid Vesicles under Osmotic Stress

Chabanon

Liedberg

et al. 2017

Biophysical Journal

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“…(iii) Does the critical lytic tension depend on the strain rate, and thus the strength of the osmotic gradient? Such questions arise beyond the present context of vesicle osmoregulation in other important scenarios where the coupling between the dissipation of osmotic energy and cellular compartmentalization has important biological ramifications (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Pulsatile lipid vesicles under osmotic stress

Chabanon

Liedberg

et al. 2016

Preprint

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The response of lipid bilayers to osmotic stress is an important part of cellular function. Recent experimental studies showed that when cell-sized giant unilamellar vesicles (GUVs) are exposed to hypotonic media, they respond to the osmotic assault by undergoing a cyclical sequence of swelling and bursting events, coupled to the membrane's compositional degrees of freedom. Here, we establish a fundamental and quantitative understanding of the essential pulsatile behavior of GUVs under hypotonic conditions by advancing a comprehensive theoretical model of vesicle dynamics. The model quantitatively captures the experimentally measured swellburst parameters for single-component GUVs, and reveals that thermal fluctuations enable rate-dependent pore nucleation, driving the dynamics of the swell-burst cycles. We further extract constitutional scaling relationships between the pulsatile dynamics and GUV properties over multiple time scales. Our findings provide a fundamental framework that has the potential to guide future investigations on the non-equilibrium dynamics of vesicles under osmotic stress.

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“…The authors of [230] have studied the wound closure in Madin-Darby canine kidney epithelial cell layers and established that submarginal cells exhibit protrusive and migratory behavior similar to that of marginal cells. In [231], the wound made in an epithelium of human cancerous melanocytes, does not exhibit this leader cell mechanism but exhibits a diffuse and noisy border. The results of [232] show that the fibroblast growth factor leads to directed migration of leader cells but do not control cell migration and coordination of the follower cells.…”

Section: Constitutive Laws For Fibrous Collagen Networkmentioning

confidence: 99%

Towards a unified approach in the modeling of fibrosis: A review with research perspectives

Amar

Bianca

2016

Physics of Life Reviews

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Pathological fibrosis is the result of a failure in the wound healing process. The comprehension and the related modeling of the different mechanisms that trigger fibrosis is a challenge of many researchers that work in the field of medicine and biology. The modern scientific analysis of a phenomenon generally consists of three major approaches: theoretical, experimental, and computational. Different theoretical tools coming from mathematics and physics have been proposed for the modeling of the physiological and pathological fibrosis. However a complete framework is missing and the development of a general theory is required. This review aims at finding a unified approach in the modeling of fibrosis diseases that takes into account the different phenomena occurring at each level: molecular, cellular and tissue. Specifically by means of a critical analysis of the different models that have been proposed in the mathematical, computational and physical biology, from molecular to tissue scales, a multiscale approach is proposed, an approach that has been strongly recommended by top level biologists in the past decades.

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Osmotic stress affects functional properties of human melanoma cell lines

Cited by 22 publications

References 26 publications

Pulsatile Lipid Vesicles under Osmotic Stress

Pulsatile Lipid Vesicles under Osmotic Stress

Pulsatile lipid vesicles under osmotic stress

Towards a unified approach in the modeling of fibrosis: A review with research perspectives

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