Osmotic fragility of red blood cells, lipid peroxidation and Ca<sup>2+</sup>-ATPase activity of placental homogenates and red blood cell ghosts in salt-loaded pregnant rats

Rojas, Deliana; Rodriguez, Freddy; Barráez, Juvell; Piñero, Sandy; Chiarello, Delia I.; Abad, Cilia; Marı́n, Reinaldo; Proverbio, Fulgencio

doi:10.3109/14767058.2014.994499

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…Table 2 shows the results obtained. Notice that as previously reported (Beauséjour et al, 2003;Rojas et al, 2015), the increase of sodium intake in pregnant rats (SLP) was accompanied by a significant decrease in placental and fetal weight. The presence of Mg-gluconate in the drinking water of control pregnant rats did not produce any significant change in the fetal parameters of the rats.…”

Section: Resultssupporting

confidence: 70%

“…These features have been interpreted as an indication of a decreased placental perfusion in the salt-loaded pregnant rats (Beauséjour et al, 2003;St-Louis et al, 2006). In our laboratory, we have shown that blood plasma, red blood cells ghosts, and placental homogenates of salt-loaded pregnant rats show an increased level of lipid peroxidation, as well as a concomitant, lowered PMCA activity (Rojas et al, 2015) which was previously shown in both red blood cell ghosts and syncytiotrophoblast plasma membranes from preeclamptic pregnant women (Matteo et al, 1998;Abad et al, 2012). Additionally, red blood cells from experimental rats displayed higher osmotic fragility compare to control rats.…”

Section: Introductionmentioning

confidence: 79%

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Effect of Mg-Gluconate on the Osmotic Fragility of Red Blood Cells, Lipid Peroxidation, and Ca2+-ATPase (PMCA) Activity of Placental Homogenates and Red Blood Cell Ghosts From Salt-Loaded Pregnant Rats

et al. 2022

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Preeclampsia (PE) is a pregnancy-specific syndrome with multisystem involvement which leads to fetal, neonatal, and maternal morbidity and mortality. A model of salt-loaded pregnant rats has been previously studied, sharing several pathological characteristics of preeclamptic women. In this study, it was compared the effects of the treatment with an oral magnesium salt, magnesium gluconate (Mg-gluconate), on the osmotic fragility of red blood cells, lipid peroxidation, and PMCA activity of placental homogenates and red blood cell ghosts in salt-loaded pregnant rats. Mg-gluconate has a higher antioxidant capacity than MgSO4 due to the presence of several hydroxyl groups in the two anions of this salt. Salt-loaded pregnant rats received 1.8% NaCl solution ad libitum as a beverage during the last week of pregnancy. On day 22nd of pregnancy, the rats were euthanized and red blood cells and placenta were obtained. Salt-loaded pregnant rats showed an increased level of lipid peroxidation and a lowered PMCA activity in placental and red blood cell ghosts, as well as an increased osmotic fragility of their red blood cells. The treatment of the salt-loaded pregnant rats with Mg-gluconate avoids the rise in the level of lipid peroxidation and the concomitant lowering of the PMCA activity of their red blood cell membranes, reaching values similar to those from control pregnant rats. Also, this treatment prevents the increase of the osmotic fragility of their red blood cells, keeping values similar to those from control pregnant rats. Mg-gluconate seems to be an important candidate for the replacement of the MgSO4 treatment of preeclamptic women.

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Section: Resultssupporting

confidence: 70%

Section: Introductionmentioning

confidence: 79%

Effect of Mg-Gluconate on the Osmotic Fragility of Red Blood Cells, Lipid Peroxidation, and Ca2+-ATPase (PMCA) Activity of Placental Homogenates and Red Blood Cell Ghosts From Salt-Loaded Pregnant Rats

et al. 2022

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“…PWH have high reactive species production in peripheral immune cells (67,68) and elevated lipid peroxidation products in plasma (69). We and others have previously shown that RBCs are especially susceptible to oxidative damage by lipid peroxidation (32,33,70), resulting in release of hemoglobin (Hb), metHb, free heme, and free iron (71). In this study we also found that PWH who self-report CWP had elevated levels of carbonyl adducts in RBC membranes.…”

Section: Discussionsupporting

confidence: 65%

Heme Attenuates Endogenous Opioid Levels in Leukocytes of HIV positive individuals with Chronic Widespread Pain

Aggarwal

DeBerry

Ahmad

et al. 2020

Preprint

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The prevalence of chronic widespread pain (CWP) in people with HIV (PWH) is high, yet the underlying mechanisms are elusive. Leukocytes synthesize the endogenous opioid, β-endorphin (β-END), within their endoplasmic reticulum (ER). When released into plasma, β-END dampens nociceptive transmission by binding to opioid receptors on sensory neurons. In the present study, we hypothesized that heme-induced ER stress attenuates leukocyte levels/release of β-END, thereby increasing pain sensitivity in PWH. Results demonstrate that PWH with CWP have fragile erythrocytes, high plasma levels of cell-free heme, and impaired heme metabolism. Leukocytes from PWH with CWP also had high ER stress and low β-END compared to PWH without CWP and HIV-negative individuals with or without pain. In vitro heme exposure decreased β-END levels/secretion in murine monocytes/macrophages, which was prevented by treatment with sodium 4phenylbutyrate, an ER stress inhibitor. To mimic hemolytic effects in a preclinical model in vivo, C57BL/6 mice were injected with phenylhydrazine hydrochloride (PHZ). PHZ increased cell-free heme and ER stress, decreased leukocyte β-END levels and hindpaw mechanical sensitivity thresholds. Treatment of PHZ-injected mice with the heme scavenger, hemopexin, blocked these effects, suggesting that heme-induced ER stress and a subsequent decrease in leukocyte β-END may contribute to CWP in PWH.

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“…People with HIV have high reactive species production in peripheral immune cells [ 70 , 71 ] and elevated lipid peroxidation products in plasma [ 72 ]. We and others have previously shown that RBCs are especially susceptible to oxidative damage by lipid peroxidation [ 32 , 33 , 73 ], resulting in release of hemoglobin (Hb), metHb, free heme, and free iron [ 74 ]. In this study we also found that HIV positive individuals who self-report CWP had elevated levels of methemoglobin in plasma and carbonyl adducts in RBC membranes.…”

Section: Discussionmentioning

confidence: 99%

Heme attenuates beta-endorphin levels in leukocytes of HIV positive individuals with chronic widespread pain

et al. 2020

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The prevalence of chronic widespread pain (CWP) in people with HIV is high, yet the underlying mechanisms are elusive. Leukocytes synthesize the endogenous opioid, β-endorphin, within their endoplasmic reticulum (ER). When released into plasma, β-endorphin dampens nociception by binding to opioid receptors on sensory neurons. We hypothesized that the heme-dependent redox signaling induces ER stress, which attenuates leukocyte β-endorphins levels/release, thereby increasing pain sensitivity in people with HIV. Results demonstrated that HIV positive individuals with CWP had increased plasma methemoglobin, erythrocytes membrane oxidation, hemolysis, and low plasma heme scavenging enzyme, hemopexin, compared to people with HIV without CWP and HIV-negative individuals with or without pain. In addition, the leukocytes from people with HIV with CWP had attenuated levels of the heme metabolizing enzyme, heme oxygenase-1, which metabolizes free heme to carbon-monoxide and biliverdin. These individuals also had elevated ER stress, and low β-endorphin in leukocytes. In vitro, heme exposure or heme oxygenase-1 deletion, decreased β-endorphins in murine monocytes/macrophages. Treating cells with a carbon-monoxide donor or an ER stress inhibitor, increased β-endorphins. To mimic hemolytic effects in a preclinical model, C57BL/6 mice were injected with phenylhydrazine hydrochloride (PHZ). PHZ increased cell-free heme and ER stress, decreased leukocyte β-endorphin levels and hindpaw mechanical sensitivity thresholds. Treatment of PHZ-injected mice with hemopexin blocked these effects, suggesting that heme-induced ER stress and a subsequent decrease in leukocyte β-endorphin is responsible for hypersensitivity in people with HIV.

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Osmotic fragility of red blood cells, lipid peroxidation and Ca²⁺-ATPase activity of placental homogenates and red blood cell ghosts in salt-loaded pregnant rats

Abstract: Salt-loaded pregnant rats showed, similar to preeclamptic women, an increased level of lipid peroxidation and a lowered Ca(2+)-ATPase activity in placental and red blood cells membranes, as well as an increased osmotic fragility of the red blood cells.

Cited by 6 publications

References 27 publications

Effect of Mg-Gluconate on the Osmotic Fragility of Red Blood Cells, Lipid Peroxidation, and Ca2+-ATPase (PMCA) Activity of Placental Homogenates and Red Blood Cell Ghosts From Salt-Loaded Pregnant Rats

Effect of Mg-Gluconate on the Osmotic Fragility of Red Blood Cells, Lipid Peroxidation, and Ca2+-ATPase (PMCA) Activity of Placental Homogenates and Red Blood Cell Ghosts From Salt-Loaded Pregnant Rats

Heme Attenuates Endogenous Opioid Levels in Leukocytes of HIV positive individuals with Chronic Widespread Pain

Heme attenuates beta-endorphin levels in leukocytes of HIV positive individuals with chronic widespread pain

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Osmotic fragility of red blood cells, lipid peroxidation and Ca2+-ATPase activity of placental homogenates and red blood cell ghosts in salt-loaded pregnant rats

Abstract: Salt-loaded pregnant rats showed, similar to preeclamptic women, an increased level of lipid peroxidation and a lowered Ca(2+)-ATPase activity in placental and red blood cells membranes, as well as an increased osmotic fragility of the red blood cells.

Cited by 6 publications

References 27 publications

Effect of Mg-Gluconate on the Osmotic Fragility of Red Blood Cells, Lipid Peroxidation, and Ca2+-ATPase (PMCA) Activity of Placental Homogenates and Red Blood Cell Ghosts From Salt-Loaded Pregnant Rats

Effect of Mg-Gluconate on the Osmotic Fragility of Red Blood Cells, Lipid Peroxidation, and Ca2+-ATPase (PMCA) Activity of Placental Homogenates and Red Blood Cell Ghosts From Salt-Loaded Pregnant Rats

Heme Attenuates Endogenous Opioid Levels in Leukocytes of HIV positive individuals with Chronic Widespread Pain

Heme attenuates beta-endorphin levels in leukocytes of HIV positive individuals with chronic widespread pain

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Osmotic fragility of red blood cells, lipid peroxidation and Ca²⁺-ATPase activity of placental homogenates and red blood cell ghosts in salt-loaded pregnant rats