This study examined the vacuum drying of jaboticaba berries at temperatures ranging from 40 to 70 °C. Prior to drying, the berries underwent osmotic dehydration using 70% sugar and 10% salt solutions separately. The drying behavior of the osmotically treated berries differed at higher temperatures (60−70 °C). Instead of the usual two falling rate periods, the osmotically treated berries displayed an increasing rate, followed by a falling rate period. The Midilli−Kucuk model satisfactorily described the drying kinetics, and the moisture diffusivity was approximately 4 × 10 −10 m 2 /s, increasing to 1 × 10 −9 m 2 /s at 70 °C. The drying temperature and duration influenced the phenolic compounds, including flavonoids and anthocyanins, as well as the reducing power of the berries. Flavonoids (0.97−1.56 mg of QE/g) were more susceptible to extended drying duration than temperature, while salttreated berries could prevent rapid degradation of anthocyanins (0.25−0.51 mg of C3G/g) better than sugar-treated berries (0.12− 0.50 mg of C3G/g). Nontreated berries demonstrated an IC 50 value of 303.50 μg/mL against the proliferation of lung cancer cells.