Osmotic deformation of red blood cell ghosts induced by carbohydrates

Chang, Taihyun; Sharpless, Philip B.; Davenport, Deborah A.; Radunsky, Michael B.; Yu, Hyuk

doi:10.1016/0005-2736(83)90027-5

Cited by 6 publications

(1 citation statement)

References 17 publications

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“…Thus the ghosts shrank to a greater extent in solutions containing raffinose and sucrose than in media containing isosmotic amounts of glyceraldehyde and erythritol. Osmotic pressure/volume curves obtained using different organic solutes gave different values for the nonosmotic volume (the intercept on the ordinate axis) in each case (Chang et al, 1983). Such observations are in contrast to a recent report for mouse fibroblast cells where, although osmotic pressure/volume responses were found to be dependent upon the nature of the external solute (in this case the ionic salts KC1, NaC1, LiC1 and CaC12 were used), the curves all produced the same nonosmotic volume (Raaphorst & Kruuv, 1979).…”

Section: Introductionmentioning

confidence: 99%

Transient breakdown in the selective permeability of the plasma membrane ofChlorella emersonii in response to hyperosmotic shock: Implications for cell water relations and osmotic adjustment

Reed

1984

J. Membrain Biol.

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Summary.In osmotic experiments involving cells of the euryhaline unicellular green alga Chlorella emersonii exposed to hyperosmotic stress by immersion in a range of low molecular weight organic and inorganic solutes, a temporary breakdown in the selective permeability of the plasma membrane was observed during the initial phase of transfer to media of high osmotic strength (up to 2000 mosmol kg 1). Thus, although the cells appeared to obey the Boyle-van't Hoff relationship in all cases, showing approximately linear changes in volume (at high salinity) as a function of the reciprocal of the external osmotic pressure, the extent of change was least for the triitols, propylene glycol and glycerol, intermediate for glucose, sorbitol, NaC1 and KC1, with greatest changes in media containing the disaccharides sucrose and maltose. In NaCl-treated cells, uptake of external solute and loss of internal ions was observed in response to hyperosmotic treatment while sucrose-treated cells showed no significant uptake of external solute, although loss of intracellular K + was observed. These observations suggest that the widely used technique of estimating cellular turgor, and osmotic/nonosmotic volume by means of the changes in volume that occur upon transfer to media containing increasing amounts of either a low molecular weight organic solute or an inorganic salt may be subject to error. The assumption that all algal cells behave as "ideal osmometers," with outer membranes that are permeable to water but not to solutes, during the course of such experiments is therefore incorrect, and the data need to be adjusted to take account of hyperosmotically induced external solute penetration and/or loss of intracellular osmotica before meaningful estimates of cell turgor and osmotic volume can be obtained.Key Words Chlorella emersonii . unicellular alga 9 osmotic responses -cell volume 9 membrane permeability 9 hyperosmotic shock 9 osmometric behavior

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Section: Introductionmentioning

confidence: 99%

Transient breakdown in the selective permeability of the plasma membrane ofChlorella emersonii in response to hyperosmotic shock: Implications for cell water relations and osmotic adjustment

Reed

1984

J. Membrain Biol.

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Acute toxicity of methyl isocyanate in rabbit: In vitro and in vivo effects on rabbit erythrocyte membrane

Jeevaratnam

Vaidyanathan

1992

Arch. Environ. Contam. Toxicol.

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Methyl isocyanate (MIC) interaction with the rabbit erythrocyte membrane increased the fluidity of the membrane and decreased the osmotic fragility of erythrocytes both in vitro and in vivo in rabbits intoxicated with MIC subcutaneously. MIC inhibited both acetylcholinesterase (AChE) and adenosine triphosphatase (ATPase) activities of erythrocytes dose-dependently in vitro, while in vivo a decreased trend in ATPase activity with unaltered AChE activity was observed. MIC also caused significant decrease in plasma sodium level with corresponding increase in potassium level in rabbits. The observed effects are due to MIC, per se, as the hydrolysis products of MIC, methylamine and N,N'-dimethylurea did not affect the erythrocyte fluidity and enzymes activities both in vitro and in vivo while they increased the osmotic fragility of erythrocytes in vivo in rabbits administered subcutaneously in equimolar concentration to MIC dosage. Inhibition of Na(+)-K(+)-dependent ATPase with altered permeability to cations and also probably water transport of plasma membrane due to MIC interaction are envisaged.

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Are osmotic forces involved in influenza virus-cell fusion?

Pritzen

Herrmann

1988

Bioscience Reports

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The kinetics of the fusion process of unsealed and resealed erythrocyte ghosts with influenza virus (A/PR8/34, A/Chile 1/83) were measured under hypotonic, isotonic and hypertonic conditions using a recently developed fluorescence assay (Hoekstra et al. (1984) Biochemistry 23:5675-5681]. No correlation between the external osmotic pressure and kinetics and extent of fusion was observed. Influenza viruses fuse as effectively with unsealed ghosts as with resealed ghosts. It is concluded that osmotic forces as well as osmotic swelling of cells are not necessary for virus-cell membrane fusion.

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Osmotic deformation of red blood cell ghosts induced by carbohydrates

Cited by 6 publications

References 17 publications

Transient breakdown in the selective permeability of the plasma membrane ofChlorella emersonii in response to hyperosmotic shock: Implications for cell water relations and osmotic adjustment

Transient breakdown in the selective permeability of the plasma membrane ofChlorella emersonii in response to hyperosmotic shock: Implications for cell water relations and osmotic adjustment

Acute toxicity of methyl isocyanate in rabbit: In vitro and in vivo effects on rabbit erythrocyte membrane

Are osmotic forces involved in influenza virus-cell fusion?

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