Osmoregulation and Na,K-ATPase expression in osmoregulatory organs of Scylla paramamosain

Chung, Kuei-Fang; Lin, Han

doi:10.1016/j.cbpa.2006.02.003

Cited by 70 publications

(50 citation statements)

References 35 publications

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“…While H. rubra acts as an osmoconformer at oceanic salinities (32‰), it transitions to osmoregulation at lower salinities, similar to previously studied marine euryhaline crustaceans (e.g. Zanders, 1980;Henry and Watts, 2001;Chung and Lin, 2006;Faleiros et al, 2010), including another atyid species (Born, 1968). However, 'strong' osmoregulating crustaceans with a marine ancestry such as the blue crab (Callinectes sapidus) maintain an osmotic gradient between the external medium and their hemolymph of 600 mOsm kg −1 H 2 O (Cameron, 1978;Henry, 2001).…”

Section: Discussionsupporting

confidence: 52%

“…In euryhaline crustaceans, expression of these genes usually increases dramatically in the osmoregulatory gills during comparable salinity transfers (reviewed by Havird et al, 2013). For example, NKA expression in C. granulatus increased 25-to 55-fold after transfer from SW to 45‰ and 2‰ (Luquet et al, 2005), with similar results for Scylla paramamosain (Chung and Lin, 2006), Pachygrapsus marmoratus (Jayasundara et al, 2007), C. sapidus (Serrano et al, 2007), C. maenas (Serrano and Henry, 2008;Jillette et al, 2011), Macrobrachium amazonicum (Faleiros et al, 2010) and Litopenaeus vannamei (Wang et al, 2012). Although utilized in fewer studies, CAc (but not CAg) (see Serrano and Henry, 2008), NKCC, HAT and AK (e.g.…”

Section: Research Articlementioning

confidence: 77%

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Osmoregulation in the Hawaiian anchialine shrimpHalocaridina rubra(Crustacea: Atyidae): expression of ion transporters, mitochondria-rich cell proliferation, and hemolymph osmolality during salinity transfers

Havird

Santos

Henry

2014

Journal of Experimental Biology

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Studies of euryhaline crustaceans have identified conserved osmoregulatory adaptions allowing hyper-osmoregulation in dilute waters. However, previous studies have mainly examined decapod brachyurans with marine ancestries inhabiting estuaries or tidal creeks on a seasonal basis. Here, we describe osmoregulation in the atyid Halocaridina rubra, an endemic Hawaiian shrimp of freshwater ancestry from the islands' anchialine ecosystem (coastal ponds with subsurface freshwater and seawater connections) that encounters near-continuous spatial and temporal salinity changes. Given this, survival and osmoregulatory responses were examined over a wide salinity range. In the laboratory, H. rubra tolerated salinities of 0-56‰, acting as both a hyper-and hypo-osmoregulator and maintaining a maximum osmotic gradient of ~868 mOsm kg −1 H 2 O in freshwater. Furthermore, hemolymph osmolality was more stable during salinity transfers relative to other crustaceans. Silver nitrate and vital mitochondria-rich cell staining suggest all gills are osmoregulatory, with a large proportion of each individual gill functioning in ion transport (including when H. rubra acts as an osmoconformer in seawater). Additionally, expression of ion transporters and supporting enzymes that typically undergo upregulation during salinity transfer in osmoregulatory gills (i.e. Na + /K + -ATPase, carbonic anhydrase, Na + /K + /2Cl -cotransporter, Vtype H + -ATPase and arginine kinase) were generally unaltered in H. rubra during similar transfers. These results suggest H. rubra (and possibly other anchialine species) maintains high, constitutive levels of gene expression and ion transport capability in the gills as a means of potentially coping with the fluctuating salinities that are encountered in anchialine habitats. Thus, anchialine taxa represent an interesting avenue for future physiological research.

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Section: Discussionsupporting

confidence: 52%

Section: Research Articlementioning

confidence: 77%

Osmoregulation in the Hawaiian anchialine shrimpHalocaridina rubra(Crustacea: Atyidae): expression of ion transporters, mitochondria-rich cell proliferation, and hemolymph osmolality during salinity transfers

Havird

Santos

Henry

2014

Journal of Experimental Biology

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“…NKA activity was measured for all of the 13 species but HA activity and western blotting were only done for Uca formosensis. The protein concentration assay followed the method described previously (Chung and Lin, 2006). Protein concentration was determined using a detergent compatible kit (BioRad, Hercules, CA, USA, Cat.…”

Section: Protein Extractionmentioning

confidence: 99%

“…Many organs are involved in osmoregulation, including the gills, the antennal gland, part of the intestine and the hepatopancreas. The gills are one of the most important osmoregulatory organs in aquatic crustaceans (Péqueux, 1995;Lucu and Towle, 2003;Chung and Lin, 2006). In osmoregulatory crabs, there are some functional differentiations among/within the gills.…”

Section: Introductionmentioning

confidence: 99%

V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

Tsai

Lin

2007

Journal of Experimental Biology

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125

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SUMMARY Because of their diverse habitats, crabs are excellent experimental species to study owing to the morphological changes and physiological adaptation that occur during their terrestrial invasion. Their hemolymphic osmoregulation in brackish water is crucial for a successful terrestrial invasion. Crabs can actively uptake or excrete ions upon salinity change, and the gills play a major role among the osmoregulatory organs. Several enzymes are involved in the osmoregulatory process, including Na+,K+-ATPase and V-type H+-ATPase (V-H+-ATPase). Na+,K+-ATPase is the driving force in establishing an ion gradient across the epithelial cell membrane in marine crabs. It has been reported that the osmoregulatory mechanisms in freshwater crabs are different from those in marine ones, suggesting that the driving force may come from V-H+-ATPase by generating the H+ ion gradient to facilitate the ion flow. Thirteen crab species from two families were used in this study. These crabs lived in five different habitats, including marine,intertidal, bimodal, freshwater and terrestrial habitats. The distribution of V-H+-ATPase in the 13 euryhaline crabs was revealed by histochemistry. V-H+-ATPase was localized in the apical region in crabs that could survive in the freshwater environment. We found that the freshwater and terrestrial crabs with stable Na+,K+-ATPase activity during salinity changes tended to have an apical V-H+-ATPase, whereas the intertidal ones with varying Na+,K+-ATPase activity showed a cytoplasmic V-H+-ATPase distribution. Finally, in Uca formosensis, a crab that had stable Na+,K+-ATPase activity, a significant difference in V-H+-ATPase activity between salinities was found. In conclusion, the hypothesis that V-H+-ATPase plays a crucial role in the freshwater adaptation of crabs is supported by our systemic investigation on 13 euryhaline crabs.

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“…Molecular and cellular responses of osmoregulatory enzymes, iron transport proteins, and immune metabolism-related genes have been studied in the gills of crustaceans and teleost under acute salinity stress (Henry et al 2002(Henry et al , 2003Scott et al 2004;Chung and Lin 2006;de la Vega et al 2007). Gene expression analysis has been conducted using different salinity stresses via cDNA microarray chip and expressed sequence tags (ESTs) in order to study the osmoregulation mechanism in swimming crab Portunus trituberculatus (Xu et al 2010;Xu and Liu 2011) and it has been found that ion transport processes, amino acid metabolism, and synthesis processes and chitin metabolic processes have been involved in gills osmoregulation process.…”

mentioning

confidence: 99%

Chitinase gene responses and tissue sensitivity in an intertidal mud crab (Macrophthalmus japonicus) following low or high salinity stress

Nikapitiya

Kim

Park

et al. 2015

Cell Stress and Chaperones

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Changes in salinity affect the physiological status of the marine habitat including that of the intertidal mud crab Macrophthalmus japonicus. Chitinases play significant biological roles in crustaceans such as morphogenesis, nutrient digestion, and pathogen defense. In this study, the osmoregulatory function of three chitinase gene transcripts was determined compared to seawater (SW, 31±1 psu) in M. japonicus gills and hepatopancreas under different salinities (10, 25, and 40 psu) for 1, 4, and 7 days. In SW-exposed crab, quantitative real-time PCR analysis showed chitinase 1 (Mj-chi1) and chitinase 4 (Mj-chi4) transcripts constitutively expressed in all the tested tissues with strong expression in hepatopancreas, but chitinase 5 (Mj-chi5) showed highest expression in stomach. When exposed to different salinities, Mj-chi1 showed significant up-regulation at day 4 whereas Mj-chi4 showed late up-regulation (day 7) at all the salinities in hepatopancreas. In the gills, early up-regulation (day 1) in Mj-chi1 and time-dependent late up-regulation (day 7) in Mj-chi4 at high salinity were observed. These results indicate the possibility of using Mj-chi4 as a marker against salinity changes. Moreover, our results further suggest that Mj-chi1 and Mj-chi4 transcriptions were significantly affected by changes in salinity; however, Mj-chi5 in gills was less affected by salinity and showed no effect in hepatopancreas. Thus, chitinase transcription modulations in the gills are more sensitive than hepatopancreas to salinity stress. Further, present data indicate the possible existence of different physiological roles among chitinase gene families, which need to be clarified in more detail by future biochemical and physiological functional studies.

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Osmoregulation and Na,K-ATPase expression in osmoregulatory organs of Scylla paramamosain

Cited by 70 publications

References 35 publications

Osmoregulation in the Hawaiian anchialine shrimpHalocaridina rubra(Crustacea: Atyidae): expression of ion transporters, mitochondria-rich cell proliferation, and hemolymph osmolality during salinity transfers

Osmoregulation in the Hawaiian anchialine shrimpHalocaridina rubra(Crustacea: Atyidae): expression of ion transporters, mitochondria-rich cell proliferation, and hemolymph osmolality during salinity transfers

V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

Chitinase gene responses and tissue sensitivity in an intertidal mud crab (Macrophthalmus japonicus) following low or high salinity stress

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