Osmolyte modulated enhanced rice leaf catalase activity under salt-stress

Abstract

Change in catalase activity was examined in leaves of rice plant exposed to salinity. Depending on the method of preparation of crude protein extract from leaf and the constituents of the assay medium, a significant difference in enzyme activity was recorded. Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in unstressed plants, which otherwise showed a 50% declined activity in leaf extract prepared in buffer solution and assayed in a medium depleted of these sugars. In view of the accumulation of osmolytes under saline condition, these observations suggest that the catalase activity is modulated by the osmolytes and maintains a high rate of hydrogen peroxide scavenging property in vivo and serves as the major antioxidant enzyme to scavenge the salt-induced formation of peroxide. Therefore, the salt-stress induced appearance of low activity of the enzyme under normal buffer extraction and assay conditions, as reported in literature may represent an apparent than for its real in vivo activity.

Novel insight into the molecular interaction of catalase and sucrose: A combination of in silico and in planta assays study

“…As expected Sahu et al (2010) have shown a declined rice leaf catalase activity under elevated salt-treatment, however, surprisingly, supplementation of sucrose significantly rescues salt mediated decay in catalase activity. The investigation also evidenced that catalase in rice leaf interacts with sucrose under in vivo condition and renders a protective role in maintaining high enzymatic activity thus acting as the principal enzymatic source for scavenging the stress induced production of H 2 O 2 (Sahu et al, 2010).…”

“…Plants treated with different concentrations of sucrose under salt stress condition induces metabolic pathways and induced systemic responses that reprogrammed the whole/partial catabolic and metabolic cascade related to salt tolerance and signalling (Kumar et al, 2013;Sahu et al, 2010). It was found that plants treated with sucrose activated stress related cascade and caused manifold increase in the activation of catalase activities (Fig.…”

A unique bZIP transcription factor imparting multiple stress tolerance in Rice

“…In situ detection of hydrogen peroxide (H 2 O 2 ) was performed by staining the tissue with DAB (Sigma-Aldrich) as suggested (Bindschedler et al 2006). Steady-state levels of H 2 O 2 in leaves were determined following FOX-1 method (Sahu et al 2010).…”

Investigation of the possible protective effect of Smilax fluminensis steud. leaf in mice subjected to oxidative stress by paracetamol

“…Oxidative stress is a result of the imbalance between reactive oxygen species (ROS), nitrogen (ERN) and the antioxidant defense system present in the body (Sahu et al 2010). When such a balance tends towards an excessive production of these compounds or a deficiency of the antioxidant systems, the oxidative stress condition arises, which is harmful to the cellular components and to the individuals as a whole (Carocho and Ferreira 2013).…”