OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity

Huang, Jiexue; Cai, Maohong; Long, Qizhang; Liu, Linglong; Lin, Qinlu; Jiang, Ling; Chen, Saihua; Wan, Jianmin

doi:10.1007/s11248-014-9803-2

Cited by 61 publications

(42 citation statements)

References 49 publications

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“…Based on these information, it is reasonable for us to propose that lipid peroxidation could be one of the main causes of wheat seed aging during storage and inactivated LOXs could extend the seed longevity via alleviating the damage of cell membranes and cellular proteins. Such proposition was also supported by other previous researches (Wilson and McDonald 1986;McDonald 1999;Zhang et al 2007;Huang et al 2014). …”

Section: Discussionsupporting

confidence: 87%

“…Lipid degradation was proposed as one cause of seed deterioration during storage (Takano 1993). Similar to the results from soybean, the negative relationships between LOX activity and seed longevity have also been observed in maize and rice (Li et al 2007;Song et al 2007;Jiang et al 2009;Long et al 2013;Gayen et al 2014;Huang et al 2014). For example, after storing seeds of three maize varieties with and without LOX-1, 2 at 42°C and 84 % relative humidity for a period of 15 days, the variety lacking LOX-1, 2 showed a slight change in germination, but for varieties with LOX-1, 2, an obvious decline in germination was observed, suggesting LOX-1, 2 may be a definitive factor which influences seed life span (Li et al 2007).…”

supporting

confidence: 69%

“…The relationships between LOX activity and seed longevity during AA treatment have been well studied in maize and rice (Li et al 2007;Song et al 2007;Jiang et al 2009;Long et al 2013;Gayen et al 2014;Huang et al 2014). However, there have been few reports about the effect of LOXs on wheat grain deterioration.…”

Section: Discussionmentioning

confidence: 99%

“…In rice seeds, three LOXs were detected and LOX-3 is the major isozyme component (Suzuki et al 1996). Rice lines over-expressing LOX2 showed lower seed viability, while RNAi of LOX2 caused enhanced seed longevity after accelerated aging (Huang et al 2014). Overexpressing rice LOX3 was also found accelerated the decrease in seed germination ability when the seeds were routinely stored (Long et al 2013).…”

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confidence: 99%

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Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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In higher plants, lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids and can accumulate to relatively high levels in the seeds. However, high LOX activity has often been found associated with harmful effects on the quality attributes and the longevity of harvested seeds. Here, we report the development and characterization of three homozygous T 5 transgenic lines (designated as LOX RNAi -4, 7 and 9, respectively) with LOX activity specifically reduced in the grains of common wheat. An RNAi hairpin directed under an endosperm-specific promoter was introduced into the genome of the elite wheat variety Longchun 23 using particle bombardment. Each transgenic line (LOX RNAi -4, 7 or 9) derived from separate integration event, which carried more than 20 copies of the transgene. The three LOX RNAi lines and wild-type (WT) Longchun 23 showed a similar level of LOX transcripts in the flag leaves. However, relative to WT control, LOX RNAi -4, 7 and 9 exhibited drastic decreases in the level of LOX transcripts during grain development compared with WT control. The LOX activity levels in the harvested grains of the three transgenic lines were significantly lower than that of WT control under either normal conditions or artificial aging (AA, stored at 40°C and 85 % humidity for 6 or 9 days) treatment. The whiteness values of the flour samples from LOX RNAi -4, 7 and 9 were generally and significantly higher than those of WT control regardless of the grains were stored under normal or AA conditions. Furthermore, the germination rates of LOX RNAi -4, 7 and 9 were substantially higher than those of WT control under AA treatment. Our data demonstrate that grainspecific reduction in LOX activity by RNAi may represent a generally applicable approach for improving the quality traits and longevity of crop seeds.Keywords Lipoxygenase Á RNA interference Á Germination rate Á Whiteness Á Wheat Zhenying Dong and Bo Feng have contributed equally to this work.Electronic supplementary material The online version of this article

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Section: Discussionsupporting

confidence: 87%

supporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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“…The expression levels of LOX1 , LOX2 and LOX3 , especially that of LOX3 , the key enzyme of seed longevity, were much higher between the low longevity varieties FH653 and H2 (Figure b). According to the results of previous studies, LOX1 was involved in tolerance of the rice plant to wounding and brown planthopper attack, whereas LOX2 and LOX3 both had negative effect on seed longevity (Huang et al, ; Long et al, ; Wang et al, ). In our study, the storable variety FH7185 also showed lower expression level of LOX2 and LOX3 .…”

Section: Resultsmentioning

confidence: 96%

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Jiang

Lian

et al. 2019

Plant Breeding

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Seed longevity is a very important characteristic controlling seed quality. However, the mechanism underlying this characteristic is poorly understood. In this study, 'FH7185', a storable rice variety, the germination rate of which was 66.63%, much higher than the control after artificial ageing under relative humidity 88% and 42°C for 21 days. Different methods were applied to reveal the involvement of proteins during ageing of rice seeds. In total, 35 differential proteins were identified from 2D‐PAGE, and 3,719 proteins from iTRAQ analysis. A comparison of these two methods showed that not all protein types could be detected on the 2D‐PAGE gels, and the dynamic range was somewhat limited. So the comprehensive proteome map from the iTRAQ analysis was used to identify the quantitatively regulated proteins that played roles in seed longevity, and found that the most marked change was the increased abundance of many metabolic enzymes, especially the ones involved in α‐linolenic acid metabolism in the embryos during ageing. OsLOX2 and OsLOX3 had a negative effect on seed longevity, which were lower in FH7185 than the control. The dehydrogenase (LOC_Os07g44430) which played a major catalytic role in lipid peroxidation, also changed significantly during ageing. Therefore, OsLOX2, OsLOX3 and Loc_OS 07G44430 may be involved in the regulation of seed longevity with a synergistic effect.

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Identification of a novel SPLIT-HULL (SPH) gene associated with hull splitting in rice (Oryza sativa L.)

Lee

et al. 2018

Theor Appl Genet

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The split-hull phenotype caused by reduced lemma width and low lignin content is under control of SPH encoding a type-2 13-lipoxygenase and contributes to high dehulling efficiency. Rice hulls consist of two bract-like structures, the lemma and palea. The hull is an important organ that helps to protect seeds from environmental stress, determines seed shape, and ensures grain filling. Achieving optimal hull size and morphology is beneficial for seed development. We characterized the split-hull (sph) mutant in rice, which exhibits hull splitting in the interlocking part between lemma and palea and/or the folded part of the lemma during the grain filling stage. Morphological and chemical analysis revealed that reduction in the width of the lemma and lignin content of the hull in the sph mutant might be the cause of hull splitting. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive gene, sph (Os04g0447100), which encodes a type-2 13-lipoxygenase. SPH knockout and knockdown transgenic plants displayed the same split-hull phenotype as in the mutant. The sph mutant showed significantly higher linoleic and linolenic acid (substrates of lipoxygenase) contents in spikelets compared to the wild type. It is probably due to the genetic defect of SPH and subsequent decrease in lipoxygenase activity. In dehulling experiment, the sph mutant showed high dehulling efficiency even by a weak tearing force in a dehulling machine. Collectively, the results provide a basis for understanding of the functional role of lipoxygenase in structure and maintenance of hulls, and would facilitate breeding of easy-dehulling rice.

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OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity

Cited by 61 publications

References 49 publications

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Identification of a novel SPLIT-HULL (SPH) gene associated with hull splitting in rice (Oryza sativa L.)

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