OsJAR1 and OsJAR2 are jasmonyl-l-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signalling

Wakuta, Shinji; Suzuki, Érika; Saburi, Wataru; Matsuura, Hideyuki; Nabeta, Kensuke; Imai, Ryozo; Matsui, Hirokazu

doi:10.1016/j.bbrc.2011.05.055

Cited by 72 publications

(64 citation statements)

References 42 publications

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“…Very recently, a Japanese group demonstrated that in fact two enzymes, OsJAR1 and OsGH3.3 (called OsJAR2 hereafter) are able to conjugate JA to Ile in crude extracts of Escherichia coli expressing the respective proteins (Wakuta et al 2011). Our own in vitro experiments using recombinant purified GST-tagged enzymes confirm this result, and additionally demonstrate that multiple amino acids can be used as a substrate by both enzymes (Svyatyna et al, manuscript in preparation).…”

Section: Photomorphogenic Responses Require Several Gh3 Enzymes In Ricesupporting

confidence: 64%

Light-dependent regulation of the jasmonate pathway

Svyatyna

Riemann

2012

Protoplasma

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Jasmonates (JAs) are plant hormones which are crucial for the response of plants to several biotic and abiotic stresses. Beside this important function, they are involved in several developmental processes throughout plant life. In this short review, we would like to summarize the recent findings about the function of JAs in photomorphogenesis with a main focus on the model plant rice. Early plant development is determined to a large extent by light. Depending on whether seedlings are raised in darkness or in light, they show a completely different appearance which led to the terms skoto- and photomorphogenesis, respectively. The different appearance depending on the light conditions has been used to screen for mutants in photoperception and signalling. By this approach, mutants for several photoreceptors and in the downstream signalling pathways could be isolated. In rice, we and others isolated mutants with a very intriguing phenotype. The mutated genes have been cloned by map-based cloning, and all of them encode for JA biosynthesis genes. The most bioactive form of JAs identified so far is the amino acid conjugate jasmonoyl-isoleucin (JA-Ile). In order to conjugate JA to Ile, an enzyme of the GH3 family, JASMONATE RESISTANT 1, is required. We characterized mutants of OsJAR1 on a physiological and biochemical level and found evidence for redundantly active enzymes in rice.

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Section: Photomorphogenic Responses Require Several Gh3 Enzymes In Ricesupporting

confidence: 64%

Light-dependent regulation of the jasmonate pathway

Svyatyna

Riemann

2012

Protoplasma

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“…Also, overexpression of rice OPR7 in Arabidopsis complemented opr3 defects, indicating rice OPR7 is a JAproducing enzyme (Tani et al, 2008). Moreover, recombinant JAR1 and JAR2 proteins showed JA-Ile-conjugating activity, and JAR1 and JAR2 are differentially induced upon wounding or pathogen challenge (Wakuta et al, 2011).…”

Section: Introductionmentioning

confidence: 92%

Disruption of OPR7 and OPR8 Reveals the Versatile Functions of Jasmonic Acid in Maize Development and Defense

et al. 2012

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Here, multiple functions of jasmonic acid (JA) in maize (Zea mays) are revealed by comprehensive analyses of JA-deficient mutants of the two oxo-phytodienoate reductase genes, OPR7 and OPR8. Single mutants produce wild-type levels of JA in most tissues, but the double mutant opr7 opr8 has dramatically reduced JA in all organs tested. opr7 opr8 displayed strong developmental defects, including formation of a feminized tassel, initiation of female reproductive buds at each node, and extreme elongation of ear shanks; these defects were rescued by exogenous JA. These data provide evidence that JA is required for male sex determination and suppression of female reproductive organ biogenesis. Moreover, opr7 opr8 exhibited delayed leaf senescence accompanied by reduced ethylene and abscisic acid levels and lack of anthocyanin pigmentation of brace roots. Remarkably, opr7 opr8 is nonviable in nonsterile soil and under field conditions due to extreme susceptibility to a root-rotting oomycete (Pythium spp), demonstrating that these genes are necessary for maize survival in nature. Supporting the importance of JA in insect defense, opr7 opr8 is susceptible to beet armyworm. Overall, this study provides strong genetic evidence for the global roles of JA in maize development and immunity to pathogens and insects.

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“…In Arabidopsis , only one protein, AtJAR1 was shown to utilize JA as a substrate [99]. In rice, two proteins were shown to produce JA-Ile in vitro, OsJAR1 and OsJAR2 [100]. The maize genome encodes five JAR1-like isoforms that group into two clusters (Figure 9).…”

Section: Ja-amino Acid Conjugationmentioning

confidence: 99%

Synthesis and Functions of Jasmonates in Maize

Borrego

Kolomiets

2016

Plants

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Of the over 600 oxylipins present in all plants, the phytohormone jasmonic acid (JA) remains the best understood in terms of its biosynthesis, function and signaling. Much like their eicosanoid analogues in mammalian system, evidence is growing for the role of the other oxylipins in diverse physiological processes. JA serves as the model plant oxylipin species and regulates defense and development. For several decades, the biology of JA has been characterized in a few dicot species, yet the function of JA in monocots has only recently begun to be elucidated. In this work, the synthesis and function of JA in maize is presented from the perspective of oxylipin biology. The maize genes responsible for catalyzing the reactions in the JA biosynthesis are clarified and described. Recent studies into the function of JA in maize defense against insect herbivory, pathogens and its role in growth and development are highlighted. Additionally, a list of JA-responsive genes is presented for use as biological markers for improving future investigations into JA signaling in maize.

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OsJAR1 and OsJAR2 are jasmonyl-l-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signalling

Cited by 72 publications

References 42 publications

Light-dependent regulation of the jasmonate pathway

Light-dependent regulation of the jasmonate pathway

Disruption of OPR7 and OPR8 Reveals the Versatile Functions of Jasmonic Acid in Maize Development and Defense

Synthesis and Functions of Jasmonates in Maize

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