OsHAK1, a High-Affinity Potassium Transporter, Positively Regulates Responses to Drought Stress in Rice

Chen, Guang; Liu, Chaolei; Gao, Zhenyu; Zhang, Yu; Jiang, Hongzhen; Zhu, Li; Ren, Deyong; Yu, Ling; Xu, Guohua; Qian, Qian

doi:10.3389/fpls.2017.01885

Cited by 85 publications

(74 citation statements)

References 74 publications

(119 reference statements)

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“…The H 2 O 2 content of leaf samples was determined following their equilibration in 5.3 mM TiCl 4 dissolved in 3.7 M H 2 SO 4 , based on the Mostofa and Fujita (2013) procedure. Leaf MDA contents were assessed following Chen et al (2017) , and leaf proline contents following Bates et al (1973) .…”

Section: Methodsmentioning

confidence: 99%

Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar

et al. 2018

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Salinity imposes a major constraint over the productivity of rice. A set of chromosome segment substitution lines (CSSLs), derived from a cross between the japonica type cultivar (cv.) Nipponbare (salinity sensitive) and the indica type cv. 9311 (moderately tolerant), was scored using a hydroponics system for their salinity tolerance at the seedling stage. Two of the CSSLs, which share a ∼1.2 Mbp stretch of chromosome 4 derived from cv. Nipponbare, were as sensitive to the stress as cv. Nipponbare itself. Fine mapping based on an F2 population bred from a backcross between one of these CSSLs and cv. 9311 narrowed this region to 95 Kbp, within which only one gene (OsHAK1) exhibited a differential (lower) transcript abundance in cv. Nipponbare and the two CSSLs compared to in cv. 9311. The gene was up-regulated by exposure to salinity stress both in the root and the shoot, while a knockout mutant proved to be more salinity sensitive than its wild type with respect to its growth at both the vegetative and reproductive stages. Seedlings over-expressing OsHAK1 were more tolerant than wild type, displaying a superior photosynthetic rate, a higher leaf chlorophyll content, an enhanced accumulation of proline and a reduced level of lipid peroxidation. At the transcriptome level, the over-expression of OsHAK1 stimulated a number of stress-responsive genes as well as four genes known to be involved in Na+ homeostasis and the salinity response (OsHKT1;5, OsSOS1, OsLti6a and OsLti6b). When the stress was applied at booting through to maturity, the OsHAK1 over-expressors out-yielded wild type by 25%, and no negative pleiotropic effects were expressed in plants gown under non-saline conditions. The level of expression of OsHAK1 was correlated with Na+/K+ homeostasis, which implies that the gene should be explored a target for molecular approaches to the improvement of salinity tolerance in rice.

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Section: Methodsmentioning

confidence: 99%

Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar

et al. 2018

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“…The impact on WD responses of manipulating root K + transporters has been addressed in few studies. Rice K + uptake transporters such as OsHAK1 and OsAKT1 have been shown to have a positive contribution to WD responses (Ahmad et al, 2016b;Chen et al, 2017). On the one hand, loss-of-function mutants are sensitive to WD and, on the other hand, overexpressors are tolerant.…”

Section: Potassium Membrane Transportmentioning

confidence: 99%

Coping With Water Shortage: An Update on the Role of K+, Cl-, and Water Membrane Transport Mechanisms on Drought Resistance

et al. 2019

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Drought is now recognized as the abiotic stress that causes most problems in agriculture, mainly due to the strong water demand from intensive culture and the effects of climate change, especially in arid/semi-arid areas. When plants suffer from water deficit (WD), a plethora of negative physiological alterations such as cell turgor loss, reduction of CO 2 net assimilation rate, oxidative stress damage, and nutritional imbalances, among others, can lead to a decrease in the yield production and loss of commercial quality. Nutritional imbalances in plants grown under drought stress occur by decreasing water uptake and leaf transpiration, combined by alteration of nutrient uptake and long-distance transport processes. Plants try to counteract these effects by activating drought resistance mechanisms. Correct accumulation of salts and water constitutes an important portion of these mechanisms, in particular of those related to the cell osmotic adjustment and function of stomata. In recent years, molecular insights into the regulation of K + , Cl-, and water transport under drought have been gained. Therefore, this article brings an update on this topic. Moreover, agronomical practices that ameliorate drought symptoms of crops by improving nutrient homeostasis will also be presented.

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“…The method used to quantify soluble protein content was as described by Zhou et al [ 20 ]. Leaf MDA contents were assessed using the protocol given by Chen et al [ 51 ]. To determine the H 2 O 2 and ROS-associated enzyme content in fully expanded leaves, leaf material was snap-frozen, ground to a powder and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

Knocking Out the Gene RLS1 Induces Hypersensitivity to Oxidative Stress and Premature Leaf Senescence in Rice

Chen

et al. 2018

IJMS

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Improving a plant’s level of tolerance to oxidative stress can frequently also enhance its tolerance to several other abiotic stresses. Here, a screen of a japonica type rice T-DNA insertion mutant library identified a highly oxidative stress-sensitive mutant. The line exhibited premature leaf senescence, starting at the three-leaf stage, and the symptoms were particularly severe from the five-leaf stage onwards. The leaves progressively lost chlorophyll, suffered protein degradation and were compromised with respect to their photosynthetic activity; their leaf mesophyll and bulliform cells became shrunken, and several senescence-associated genes (SAGs), senescence-associated transcription factor genes (SATFs) and autophagy-related genes (ATGs) were progressively up-regulated. The product of the gene inactivated by the mutation, identified via positional cloning, was putatively a ubiquitin-conjugating enzyme. The gene was denoted here as RLS1 (reactive oxygen species-sensitive leaf senescence1). The phenotype of plants in which RLS1 was knocked down using RNA interference was comparable to that of the rls1 mutant. A comparative analysis of the knock-out line and the wild type leaves showed that the former accumulated more hydrogen peroxide and more malondialdehyde, expressed a heightened level of superoxide dismutase activity and a decreased level of catalase activity, and exhibited an altered transcriptional profile with respect to several SAGs, SATFs and ATGs, and that these effects were magnified when the plants were exposed to oxidative stress. The product of RLS1 is presumed to be a critical component of the rice oxidative stress response and is involved in ROS (reactive oxygen species)-mediated leaf senescence.

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OsHAK1, a High-Affinity Potassium Transporter, Positively Regulates Responses to Drought Stress in Rice

Cited by 85 publications

References 74 publications

Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar

Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar

Coping With Water Shortage: An Update on the Role of K+, Cl-, and Water Membrane Transport Mechanisms on Drought Resistance

Knocking Out the Gene RLS1 Induces Hypersensitivity to Oxidative Stress and Premature Leaf Senescence in Rice

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