OsEIL1 and OsEIL2, two master regulators of rice ethylene signaling, promote the expression of ROS scavenging genes to facilitate coleoptile elongation and seedling emergence from soil

Qiao, Jinzhu; Quan, Ruidang; Wang, Juan; Li, Yuxiang; Xiao, Dinglin; Zhao, Zihan; Huang, Rongfeng; Qin, Hua

doi:10.1016/j.xplc.2023.100771

Cited by 4 publications

(4 citation statements)

References 68 publications

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“…The POD gene PER1A regulated rice seed germinability by the bZIP23 –PER1A complex via the ABA signaling pathway, further emphasizing the role of POD in SD release and germination and its association with the ABA signaling pathway [ 26 ]. More recently, two ethylene signaling regulators, OsEIL1 and OsEIL2, directly mediated the expressions of four POD genes ( OsPRX37 , OsPRX81 , OsPRX82 , and OsPRX88 ) and facilitated rice coleoptile growth by scavenging ROS [ 35 ]. However, the specific function of POD in regulating wheat SD and germination remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Gao,

Jiang,

Yang

et al. 2024

BMC Plant Biol

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Background Class III peroxidases (PODs) perform crucial functions in various developmental processes and responses to biotic and abiotic stresses. However, their roles in wheat seed dormancy (SD) and germination remain elusive. Results Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests were performed using the transgenic Arabidopsis and rice lines as well as wheat mutant mutagenized with ethyl methane sulfonate (EMS) in Jing 411 (J411) background. These results indicated that TaPer12-3A negatively regulated SD and positively mediated germination. Further studies showed that TaPer12-3A maintained H2O2 homeostasis by scavenging excess H2O2 and participated in the biosynthesis and catabolism pathways of gibberellic acid and abscisic acid to regulate SD and germination. Conclusion These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also provide a target gene for breeding wheat varieties with high pre-harvest sprouting resistance by gene editing technology.

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Section: Discussionmentioning

confidence: 99%

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Gao,

Jiang,

Yang

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…The POD gene PER1A regulated rice seed germinability by the bZIP23-PER1A complex via the ABA signaling pathway, further emphasizing the role of POD in SD release and germination and its association with the ABA signaling pathway [26]. More recently, two ethylene signaling regulators, OsEIL1 and OsEIL2, directly mediated the expressions of four POD genes (OsPRX37, OsPRX81, OsPRX82, and OsPRX88) and facilitated rice coleoptile growth by scavenging ROS [37]. However, the speci c function of POD in controlling wheat SD and germination remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Gao,

Jiang,

Yang

et al. 2023

Preprint

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Background Class III peroxidases (PODs) perform crucial functions in various developmental processes and responses to biotic and abiotic stresses. However, their roles in wheat seed dormancy (SD) and germination remain elusive. Results Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests were performed using transgenic Arabidopsis and rice lines as well as wheat mutant mutagenized with ethyl methane sulfonate (EMS) in a Jing 411 (J411) background. These tests indicated that TaPer12-3A negatively regulated SD and positively mediated germination. Further studies showed that TaPer12-3A maintained H2O2 homeostasis by scavenging excess H2O2 and participated in the biosynthesis and catabolism of gibberellic acid and abscisic acid to regulate SD and germination. Conclusion These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also provide a target gene for breeding wheat varieties with high SD and pre-harvest sprouting resistance by gene editing technology.

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“…In rice, OsEIL1, one of the six EIN3/EIL homologs, directly activates the JA biosynthesis‐related genes encoding 12‐oxo‐PHYTODIENOIC ACID REDUCTASES , leading to increased JA accumulation and enhanced resistance to M. oryzae (Yang et al ., 2017). In addition, OsEIL1 and OsEIL2, can directly activate the ROS‐scavenging genes to decrease ROS accumulation and facilitate coleoptile elongation and seedling emergence from soil (Qiao et al ., 2023). Furthermore, OsEIL2 plays a critical role in the regulation of plant abiotic stress responses, resistance to southern rice black‐streaked dwarf virus infection and the white‐backed planthopper preference (Jin et al ., 2020; Zhao et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

OsEIL2 balances rice immune responses against (hemi)biotrophic and necrotrophic pathogens via the salicylic acid and jasmonic acid synergism

Zhao,

Zhu,

Shi

et al. 2024

New Phytologist

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Summary Plants typically activate distinct defense pathways against various pathogens. Heightened resistance to one pathogen often coincides with increased susceptibility to another pathogen. However, the underlying molecular basis of this antagonistic response remains unclear. Here, we demonstrate that mutants defective in the transcription factor ETHYLENE‐INSENSITIVE 3‐LIKE 2 (OsEIL2) exhibited enhanced resistance to the biotrophic bacterial pathogen Xanthomonas oryzae pv oryzae and to the hemibiotrophic fungal pathogen Magnaporthe oryzae, but enhanced susceptibility to the necrotrophic fungal pathogen Rhizoctonia solani. Furthermore, necrotroph‐induced OsEIL2 binds to the promoter of OsWRKY67 with high affinity, leading to the upregulation of salicylic acid (SA)/jasmonic acid (JA) pathway genes and increased SA/JA levels, ultimately resulting in enhanced resistance. However, biotroph‐ and hemibiotroph‐induced OsEIL2 targets OsERF083, resulting in the inhibition of SA/JA pathway genes and decreased SA/JA levels, ultimately leading to reduced resistance. Our findings unveil a previously uncharacterized defense mechanism wherein two distinct transcriptional regulatory modules differentially mediate immunity against pathogens with different lifestyles through the transcriptional reprogramming of phytohormone pathway genes.

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OsEIL1 and OsEIL2, two master regulators of rice ethylene signaling, promote the expression of ROS scavenging genes to facilitate coleoptile elongation and seedling emergence from soil

Cited by 4 publications

References 68 publications

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

OsEIL2 balances rice immune responses against (hemi)biotrophic and necrotrophic pathogens via the salicylic acid and jasmonic acid synergism

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