Oscillations in H+ and Ca2+ Ion Fluxes around the Elongation Region of Corn Roots and Effects of External pH

Shabala, Sergey; Newman, Ian; Morris, John

doi:10.1104/pp.113.1.111

Cited by 252 publications

(234 citation statements)

References 29 publications

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“…Ion homeostasis, especially the optimum K + /Na + ratio, is crucial for plant salt stress. Discoveries achieved by non-invasive microelectrode ion flux estimation (MIFE) techniques (Shabala et al, 1997) revealed that SA pretreatment can enhance K + retention, increase H + -ATPase activity and reduce K + loss under salt stress (Jayakannan et al, 2013). Generally speaking, the potential mechanism of SA to enhance salt tolerance lies in improving photosynthesis, enhancing antioxidant protection and maintaining the optimum K + /Na + ratio (Horváth et al, 2007;Jayakannan et al, 2013;Nazar et al, 2011).…”

Section: Phytohormone In Salt Stressmentioning

confidence: 99%

Global plant-responding mechanisms to salt stress: physiological and molecular levels and implications in biotechnology

Tang

Shao

et al. 2014

Critical Reviews in Biotechnology

292

171

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The increasing seriousness of salinization aggravates the food, population and environmental issues. Ameliorating the salt-resistance of plants especially the crops is the most effective measure to solve the worldwide problem. The salinity can cause damage to plants mainly from two aspects: hyperosmotic and hyperionic stresses leading to the restrain of growth and photosynthesis. To the adverse effects, the plants derive corresponding strategies including: ion regulation and compartmentalization, biosynthesis of compatible solutes, induction of antioxidant enzymes and plant hormones. With the development of molecular biology, our understanding of the molecular and physiology knowledge is becoming clearness. The complex signal transduction underlying the salt resistance is being illuminated brighter and clearer. The SOS pathway is the central of the cell signaling in salt stress. The accumulation of the compatible solutes and the activation of the antioxidant system are the effective measures for plants to enhance the salt resistance. How to make full use of our understanding to improve the output of crops is a huge challenge for us, yet the application of the genetic engineering makes this possible. In this review, we will discuss the influence of the salt stress and the response of the plants in detail expecting to provide a particular account for the plant resistance in molecular, physiological and transgenic fields.

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Section: Phytohormone In Salt Stressmentioning

confidence: 99%

Global plant-responding mechanisms to salt stress: physiological and molecular levels and implications in biotechnology

Tang

Shao

et al. 2014

Critical Reviews in Biotechnology

292

171

View full text Add to dashboard Cite

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“…During measurement, a computer-controlled stepper motor moved the electrodes between two positions (20 and 50 m, respectively) from the root surface in a 10 second square-wave manner. The CHART software recorded the potential difference between two positions and converted them to electrochemical potential differences using the calibrated Nernst slope of the electrodes (Shabala et al, 1997). Ion fluxes were then calculated using MIFEFLUX software.…”

Section: Recording Of Extracellular K + Fluxmentioning

confidence: 99%

“…K + -selective MIFE microelectrodes were fabricated and calibrated as described elsewhere (Shabala et al, 1997). Arabidopsis roots were immobilised as described in Shabala et al (Shabala et al, 2006).…”

Section: Recording Of Extracellular K + Fluxmentioning

confidence: 99%

Arabidopsis root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death

Demidchik

Cuin

Svistunenko

et al. 2010

Journal of Cell Science

449

369

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SummaryReactive oxygen species (ROS) are central to plant stress response, signalling, development and a multitude of other processes. In this study, the plasma-membrane hydroxyl radical (HR)-activated K + channel responsible for K + efflux from root cells during stress accompanied by ROS generation is characterised. The channel showed 16-pS unitary conductance and was sensitive to Ca 2+ , tetraethylammonium, Ba 2+ , Cs + and free-radical scavengers. The channel was not found in the gork1-1 mutant, which lacks a major plasma-membrane outwardly rectifying K + channel. In intact Arabidopsis roots, both HRs and stress induced a dramatic K + efflux that was much smaller in gork1-1 plants. Tests with electron paramagnetic resonance spectroscopy showed that NaCl can stimulate HR generation in roots and this might lead to K + -channel activation. In animals, activation of K + -efflux channels by HRs can trigger programmed cell death (PCD). PCD symptoms in Arabidopsis roots developed much more slowly in gork1-1 and wild-type plants treated with K + -channel blockers or HR scavengers. Therefore, similar to animal counterparts, plant HR-activated K + channels are also involved in PCD. Overall, this study provides new insight into the regulation of plant cation transport by ROS and demonstrates possible physiological properties of plant HR-activated K + channels.

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“…Net Ca 2+ flux was measured by Xu-Yue Science & Technology Co. (www.xuyue.net) using the noninvasive, scanning ion-selective electrode technique as described previously (Shabala et al, 2000). The data obtained by the ion-selective probe technique were analyzed with Excel spreadsheet to Fluo-3/AM ester was loaded into pollen tubes at low temperature in the dark at a final concentration of 10 mM as described.…”

Section: Measurement Of Extracellular Ca 2+ Influxmentioning

confidence: 99%

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

Chen

et al. 2008

Plant Physiology

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Ca 2+ -calmodulin (Ca 2+ -CaM) is a critical molecule that mediates cellular functions by interacting with various metabolic and signaling pathways. However, the protein expression patterns and accompanying serial cytological responses in Ca 2+ -CaM signaling deficiency remain enigmatic. Here, we provide a global analysis of the cytological responses and significant alterations in protein expression profiles after trifluoperazine treatment in Picea meyeri, which abrogates Ca 2+ -CaM signaling. Ninety-three differentially displayed proteins were identified by comparative proteomics at different development stages and were assigned to different functional categories closely related to tip growth machinery. The inhibition of Ca 2+ -CaM signaling rapidly induced an increase in extracellular Ca 2+ influx, resulting in dramatically increased cytosolic Ca 2+ concentrations and ultrastructural abnormalities in organelles as the primary responses. Secondary and tertiary alterations included actin filament depolymerization, disrupted patterns of endocytosis and exocytosis, and cell wall remodeling, ultimately resulting in perturbed pollen tube extension. In parallel with these cytological events, time-course experiments revealed that most differentially expressed proteins showed time-dependent quantitative changes (i.e. some signaling proteins and proteins involved in organelle functions and energy production changed first, followed by alterations in proteins related to cytoskeletal organization, secretory pathways, and polysaccharide synthesis). Taken together, Ca 2+ -CaM dysfunction induced serial cytological responses and temporal changes in protein expression profiles, indicating the pivotal role of Ca 2+ -CaM in the regulation of tip growth machinery.

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Oscillations in H+ and Ca2+ Ion Fluxes around the Elongation Region of Corn Roots and Effects of External pH

Cited by 252 publications

References 29 publications

Global plant-responding mechanisms to salt stress: physiological and molecular levels and implications in biotechnology

Global plant-responding mechanisms to salt stress: physiological and molecular levels and implications in biotechnology

Arabidopsis root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

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