OsbHLH057 targets the AATCA cis-element to regulate disease resistance and drought tolerance in rice

Liu, Jiazong; Shen, Yanting; Cao, Hongxiang; He, Kai; Zhang, Chu; Li, Ning

doi:10.1007/s00299-022-02859-w

Cited by 21 publications

(18 citation statements)

References 46 publications

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“…Transgenic rice plants overexpressing OsERF83 showed substantial inhibition of lesion formation following rice blast infection ( Tezuka et al., 2018 ). Overexpression of OsbHLH057 enhanced disease resistance and drought tolerance in rice ( Liu et al., 2022 ). Our results showed that specific TFs were significantly higher expression in the RC than in the SC.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome and metabolome analyses of cherry leaves spot disease caused by Alternaria alternata

et al. 2023

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Alternaria alternata is a necrotrophic fungal pathogen with a broad host range that causes widespread and devastating disease in sweet cherry (Prunus avium). We selected a resistant cultivar (RC) and a susceptible cultivar (SC) of cherry and used a combined physiological, transcriptomic, and metabolomic approach to investigate the molecular mechanisms underlying the plant’s resistance to A. alternata, of which little is known. We found that A. alternata infection stimulated the outbreak of reactive oxygen species (ROS) in cherry. The responses of the antioxidant enzymes and chitinase to disease were observed earlier in the RC than in the SC. Moreover, cell wall defense ability was stronger in the RC. Differential genes and metabolites involved in defense responses and secondary metabolism were primarily enriched in the biosynthesis of phenylpropanoids, tropane, piperidine and pyridine alkaloids, flavonoids, amino acids, and α-linolenic acid. Reprogramming the phenylpropanoid pathway and the α-linolenic acid metabolic pathway led to lignin accumulation and early induction of jasmonic acid signaling, respectively, in the RC, which consequently enhanced antifungal and ROS scavenging activity. The RC contained a high level of coumarin, and in vitro tests showed that coumarin significantly inhibited A. alternata growth and development and had antifungal effect on cherry leaves. In addition, differentially expressed genes encoding transcription factors from the MYB, NAC, WRKY, ERF, and bHLH families were highly expressed, they could be the key responsive factor in the response of cherry to infection by A. alternata. Overall, this study provides molecular clues and a multifaceted understanding of the specific response of cherry to A. alternata.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome and metabolome analyses of cherry leaves spot disease caused by Alternaria alternata

et al. 2023

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“…Taken together, we identified a novel gene, bHLH57 , which acted as a positive regulator of chilling tolerance through enhancing the trehalose synthesis, ROS metabolism and CBFs/DREBs‐ dependent pathways. Previous study showed that bHLH57 functions as transcriptional activator (Liu et al, 2022), thus, we proposed a model of chilling tolerance regulation in rice mediated by transcriptional activation of bHLH57 (Figure 10), which will be beneficial for people to understand the roles of bHLH transcription factor in plant chilling stress response and grain yield improvement. Consequently, bHLH57 is a potential target to modify rice chilling tolerance and grain yield improvement in rice breeding programmes.…”

Section: Discussionmentioning

confidence: 99%

bHLH57 confers chilling tolerance and grain yield improvement in rice

Zhang

Xiang

et al. 2022

Plant Cell & Environment

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Chilling stress has become a major limiting factor that reduces crop productivity worldwide. In this study, we identified a new gene bHLH57, whose product enhances chilling tolerance in rice at diverse developmental stages. bHLH57 was mainly expressed in leaves and anthers, and its protein was targeted to the nucleus. Overexpression of bHLH57 enhanced chilling tolerance by increasing trehalose synthesis, whereas its mutants by CRISPR/Cas9‐mediated mutagenesis were more sensitive to chilling and had reduced trehalose. Meanwhile, bHLH57 may regulate ROS metabolism and CBFs/DREBs‐ dependent pathways in response to chilling stress. In addition, the overexpression of bHLH57 resulted in increased grain yield under normal and chilling conditions, however, the disruption of bHLH57 displayed decreased grain size and seed setting rate, thus reduced grain yield. Phylogenetic and nucleotide diversity analyses suggested that bHLH57 is relatively conserved in monocotyledons, and may be selected during indica populations adaptation. Taken together, we have identified a new bHLH regulator involved rice chilling tolerance and grain yield, and provide a potential target gene for improving chilling tolerance and grain yield of rice.

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“…In addition, OsWIH2 , which is a drought induced WIH gene, was activated by OsbHLH130, and the overexpression of OsbHLH130 resulted in substantially higher drought tolerance via its participation in cuticular wax biosynthesis, with reductions in the water loss rate and ROS accumulation ( Figure 2 ) [ 137 ]. OsbHLH057 targeted the AATCA cis -element, in the promoter of Os2H16 , a gene responding to fungal attack in rice, and overexpression of OsbHLH057 enhanced rice disease resistance to fungus Rhizoctonia solani and drought tolerance [ 138 ]. In sorghum, a typical bHLH gene, SbbHLH85 , plays a key role in the root development, and its overexpression increased Na + absorption, which indicates that SbbHLH85 might play a negative regulatory role in salt tolerance [ 87 ].…”

Section: The Bhlh Transcription Factor Family In Plantsmentioning

confidence: 99%

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Zuo

Lee

Kang

2023

IJMS

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Plant basic helix-loop-helix (bHLH) transcription factors are involved in many physiological processes, and they play important roles in the abiotic stress responses. The literature related to genome sequences has increased, with genome-wide studies on the bHLH transcription factors in plants. Researchers have detailed the functionally characterized bHLH transcription factors from different aspects in the model plant Arabidopsis thaliana, such as iron homeostasis and abiotic stresses; however, other important economic crops, such as rice, have not been summarized and highlighted. The bHLH members in the same subfamily have similar functions; therefore, unraveling their regulatory mechanisms will help us to identify and understand the roles of some of the unknown bHLH transcription factors in the same subfamily. In this review, we summarize the available knowledge on functionally characterized bHLH transcription factors according to four categories: plant growth and development; metabolism synthesis; plant signaling, and abiotic stress responses. We also highlight the roles of the bHLH transcription factors in some economic crops, especially in rice, and discuss future research directions for possible genetic applications in crop breeding.

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OsbHLH057 targets the AATCA cis-element to regulate disease resistance and drought tolerance in rice

Cited by 21 publications

References 46 publications

Comparative transcriptome and metabolome analyses of cherry leaves spot disease caused by Alternaria alternata

Comparative transcriptome and metabolome analyses of cherry leaves spot disease caused by Alternaria alternata

bHLH57 confers chilling tolerance and grain yield improvement in rice

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

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