Oryza sativa class III peroxidase (OsPRX38) overexpression in Arabidopsis thaliana reduces arsenic accumulation due to apoplastic lignification

Kidwai, Maria; Dhar, Yogeshwar Vikram; Gautam, Neelam; Tiwari, Madhu; Ahmad, Iffat Zareen; Asif, Mehar Hasan; Chakrabarty, Debasis

doi:10.1016/j.jhazmat.2018.09.029

Cited by 94 publications

(39 citation statements)

References 78 publications

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“…In higher plants, class III PRXs comprise a large gene family, the members of which have been reported to participate in plant’s response to abiotic stresses [ 16 ]. For example, the class III PRX gene OsPRX38 in O. sativa reportedly improved Arabidopsis arsenic (As) tolerance by activating the antioxidant system (SOD, PRX, and GST) and scavenging H 2 O 2 [ 45 ]. In tobacco, it has been shown that the overexpression of the class III PRX gene AtPrx64 in A. thaliana improved the plant’s aluminum (Al) tolerance by increasing root growth and scavenging the accumulation of Al and ROS [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

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A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

Yan

et al. 2020

BMC Plant Biol

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Background: Salt and drought are the main abiotic stresses that restrict the yield of crops. Peroxidases (PRXs) are involved in various abiotic stress responses. Furthermore, only few wheat PRXs have been characterized in the mechanism of the abiotic stress response. Results: In this study, a novel wheat peroxidase (PRX) gene named TaPRX-2A, a member of wheat class III PRX gene family, was cloned and its response to salt stress was characterized. Based on the identification and evolutionary analysis of class III PRXs in 12 plants, we proposed an evolutionary model for TaPRX-2A, suggesting that occurrence of some exon fusion events during evolution. We also detected the positive selection of PRX domain in 13 PRXs involving our evolutionary model, and found 2 or 6 positively selected sites during TaPRX-2A evolution. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results showed that TaPRX-2A exhibited relatively higher expression levels in root tissue than those exhibited in leaf and stem tissues. TaPRX-2A expression was also induced by abiotic stresses and hormone treatments such as polyethylene glycol 6000, NaCl, hydrogen peroxide (H 2 O 2), salicylic acid (SA), methyljasmonic acid (MeJA) and abscisic acid (ABA). Transgenic wheat plants with overexpression of TaPRX-2A showed higher tolerance to salt stress than wild-type (WT) plants. Confocal microscopy revealed that TaPRX-2A-eGFP was mainly localized in cell nuclei. Survival rate, relative water content, and shoot length were higher in TaPRX-2A-overexpressing wheat than in the WT wheat, whereas root length was not significantly different. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were enhanced in TaPRX-2Aoverexpressing wheat compared with those in the WT wheat, resulting in the reduction of reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content. The expression levels of downstream stress-related genes showed that RD22, TLP4, ABAI, GST22, FeSOD, and CAT exhibited higher expressions in TaPRX-2A-overexpressing wheat than in WT under salt stress. Conclusions: The results show that TaPRX-2A plays a positive role in the response to salt stress by scavenging ROS and regulating stress-related genes.

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Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that class III PRXs can improve stress tolerance by regulating the ROS balance in plants. For example, OsPRX38 improves Arabidopsis As tolerance by activating the antioxidant system and scavenging H 2 O 2 [ 45 ] . AtPrx64 improves the plant’s Al tolerance by scavenging ROS accumulation [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

Yan

et al. 2020

BMC Plant Biol

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“…As Prxs might be involved with lignification, they need to be mentioned when discussing ways to improve biomass and to facilitate its conversion. In this sense, OsPrx38 overexpression in A. thaliana increased biomass and seed yield under arsenic stress [120], which could be an alternative pathway to be engineered.…”

Section: Applicative Aspects Of Research On Cwpsmentioning

confidence: 99%

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa

Calderan-Rodrigues

Fonseca

Moraes

et al. 2019

IJMS

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Plant cell walls mostly comprise polysaccharides and proteins. The composition of monocots’ primary cell walls differs from that of dicots walls with respect to the type of hemicelluloses, the reduction of pectin abundance and the presence of aromatic molecules. Cell wall proteins (CWPs) differ among plant species, and their distribution within functional classes varies according to cell types, organs, developmental stages and/or environmental conditions. In this review, we go deeper into the findings of cell wall proteomics in monocot species and make a comparative analysis of the CWPs identified, considering their predicted functions, the organs analyzed, the plant developmental stage and their possible use as targets for biofuel production. Arabidopsis thaliana CWPs were considered as a reference to allow comparisons among different monocots, i.e., Brachypodium distachyon, Saccharum spp. and Oryza sativa. Altogether, 1159 CWPs have been acknowledged, and specificities and similarities are discussed. In particular, a search for A. thaliana homologs of CWPs identified so far in monocots allows the definition of monocot CWPs characteristics. Finally, the analysis of monocot CWPs appears to be a powerful tool for identifying candidate proteins of interest for tailoring cell walls to increase biomass yield of transformation for second-generation biofuels production.

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“…regulating plant growth and development (Beltramo et al, 2012, Cosio et al, 2009, Mei et al, 2009; responses towards pathogen defence (Almagro et al, 2008) and abiotic stress responses (Llorente et al, 2002, Klok et al, 2002, Kidwai et al, 2019, Abercrombie et al, 2008. Interestingly, a subset of Class III peroxidases have been shown to be induced in rice, following infection with Magnaporthe grisea, a causal agent of rice blast disease (Sasaki et al, 2004).…”

Section: Inferred Changes To Sa Metabolism In Nahg Plants During Infementioning

confidence: 99%

“…Recently Zhu et al (2019) identified151 Brachypodium peroxidases (BdPrx), using bioinformatic approaches and subsequently classified them into 15 subfamily group based on gene structure and protein sequence similarity. Subfamilies 10, 12 and 14 are predicted to have roles in low oxygen responses (Klok et al, 2002), arsenic tolerance (Kidwai et al, 2019), and plant development (Sundaravelpandian et al, 2013, Hruz et al, 2008 and defence respectively based on homology to characterised peroxidases in rice and Arabidopsis. Out of the 151 BdPrxs identified, we found that 39 were differentially expressed between one or more comparisons with a fold change expression cut off log2 2 (Supplementary table 3.2).…”

Section: Inferred Changes To Sa Metabolism In Nahg Plants During Infementioning

confidence: 99%

Characterising candidate genes for roles in Fusarium pseudograminearum-host interactions in the model cereal Brachypodium distachyon

Turner¹

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Fusarium Crown Rot (FCR) is a crop disease of global concern, causing yield losses and grain quality reduction in wheat and barley. In Australia, FCR is predominantly caused by the fungal pathogen Fusarium pseudograminearum (Fp) and costs the Australian wheat industry approximately AU$88 million dollars annually. Fp has a biphasic lifestyle and hence is classified as a hemi-biotrophic pathogen. Generally, during initial infection and colonisation of the host Fp is thought to be biotrophic and sources nutrition from living host tissue, whereas at later stages of infection, Fp switches to a necrotrophic lifestyle and sources nutrition from dead host tissue. Additionally, Fp can live saprophytically on residual plant material in the soil and produce spores that can remain viable for up to 16 months. Current control measures are focussed on reducing inoculum load. However, due to environmental and socioeconomic factors these can be difficult to implement. While the use of a highly

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Oryza sativa class III peroxidase (OsPRX38) overexpression in Arabidopsis thaliana reduces arsenic accumulation due to apoplastic lignification

Cited by 94 publications

References 78 publications

A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa

Characterising candidate genes for roles in Fusarium pseudograminearum-host interactions in the model cereal Brachypodium distachyon

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