Orthotopic Ovarian Grafts in the Golden Hamster

Parrott, Delphine M. V.

doi:10.1677/joe.0.0190126

Cited by 16 publications

(8 citation statements)

References 3 publications

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“…Live young have been obtained from fresh and frozen ovarian tissue grafted to orthotopic or heterotopic locations in females (Parrott 1959, 1960, Carroll et al 1990, Gosden et al 1994, Gunasena et al 1997, Baird et al 1999, Candy et al 2000, Salle et al 2002, Snow et al 2002. As grafts at heterotopic locations do not allow spontaneous conception, the oocytes have to be harvested for IVF and embryo transfer.…”

Section: Discussionmentioning

confidence: 99%

“…The reason for this is unclear. Allo-and autografts at orthotopic sites do, however, respond to endogenous gonadotrophins as they undergo spontaneous ovulation in vivo as evidenced by the presence of corpora lutea in the grafts and birth of live young (Parrott 1959, 1960, Gosden et al 1994, Jenkin et al 1996, Gunasena et al 1997, Candy et al 2000, Salle et al 2002, Petroianu et al 2002.…”

Section: Groupmentioning

confidence: 99%

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Offspring produced from heterotopic ovarian allografts in male and female recipient mice

Waterhouse¹,

Cox²,

Snow³

et al. 2004

Reproduction

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Studies on human ovarian xenografts and mouse allografts indicate that the male hormonal milieu and exogenous gonadotrophin administration stimulate antral follicle growth. However, it is not known whether oocytes produced under these conditions are developmentally competent. The objective of our study was to evaluate the developmental competence of oocytes produced in heterotopic mouse ovarian grafts placed in male and female recipient mice. Gonadotrophins were 7.5 IU pregnant mare serum gonadotrophin (PMSG) alone or 7.5 IU PMSG and 7.5 IU human chorionic gonadotrophin or were not given prior to oocyte collection. The developmental competence of oocytes was assessed by performing in vitro fertilisation and embryo transfer to recipients. When no gonadotrophins were given the cleavage rate was similar for oocytes collected from ovarian grafts in male and female recipients. Gonadotrophin treatment significantly (P < 0.05) increased two-cell formation by oocytes grown in female graft recipients but not in male recipients. Implantation rates, fetal development and the birth of live young were unaffected by the sex of the graft recipient or gonadotrophin treatment. Live offspring were produced from oocytes collected from ovarian grafts in male and female recipients treated with or without gonadotrophins. In conclusion, this work has shown that the hormonal environment of male mice can support the growth of oocytes in ovarian allografts and that these oocytes can produce live offspring.

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Section: Discussionmentioning

confidence: 99%

Section: Groupmentioning

confidence: 99%

Offspring produced from heterotopic ovarian allografts in male and female recipient mice

Waterhouse¹,

Cox²,

Snow³

et al. 2004

Reproduction

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“…20 Brief, ovaries was finely minced and incubated in collagenase II (3 mg/ml) 1640 medium for 45 min at 378C. The ratio of digest solution to ovarian tissue was 4:1.…”

Section: Apoptosis Assaymentioning

confidence: 99%

Cryopreservation of vascularized ovary: An evaluation of histology and function in rats

et al. 2008

Microsurgery

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Cryopreservation of organs has been investigated to sustain the reproductive function of patients undergoing sterilizing chemotherapy and radiotherapy or reproductive surgery. A modified protocol for whole organ cryopreservation was described and the outcome of cryopreservative ovaries was evaluated, and apoptosis of cryopreservative cells stored for different time period and the viability of cryopreserved cells stored at different temperature was examined in rats. Lewis rat ovarian grafts were perfused for 30 min at 0.35 ml/min with M2 medium containing 0.1M fructose and increasing concentrations of 0-1.5M dimethylsulfoxide, cooled to -140 degrees C controlled by a computerized program, and stored in liquid nitrogen (-196 degrees C) for 24 hours. After being thawed, ovaries were transplanted to syngeneic recipients after bilateral oophorectomy. Graft functions were monitored postoperatively. The major findings were that: 1) A 100% survival rate of rat ovaries was achieved in this study. Ovarian hormone secretion recovered in 80% rats which had received cryopreservative ovarian grafts. Postoperative serum estradiol levels in the cryopreservative graft group were lower than in the sham surgery control, but much higher than in the bilateral oophorectomy group. 2) Histological examination of cryopreservative ovarian grafts showed preantral and antral follicles. Two gestations were obtained. 3) Estradiol levels remained low in ovariectomized rats while in the oophorectomized rats given cryopreservative ovarian grafts levels started to rise after 14 +/- 3 days. 4) The average viability in the cells from cryopreservative ovary organ (-196 degrees C) was about 71 +/- 18% compared to 90 +/- 9% of fresh cells. This success should encourage further improvement of cryopreservative techniques for large organs.

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“…Live births have been reported after orthotopic ovarian transplantation in the hamster (Parrott, 1959), mouse (Jones and Krohn, 1960), rabbit (Winston and McClure Browne, 1974) and sheep (Gosden et al, 1994a). In the human there is one report of a live birth after orthotopic transplantation (Morris, 1895), and heterotopic transplantation has been used to restore menstrual cyclicity (Davidson, 1912).…”

Section: Introductionmentioning

confidence: 99%

Ovary and ovulation: Follicular development in cryopreserved marmoset ovarian tissue after transplantation

1995

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Pieces of marmoset ovary were frozen by slow cooling in 1.5 M dimethylsulphoxide. The follicles in fresh and frozen tissue were counted and examined for morphological appearance in stained serial sections. The proportion of normal follicles was similar in fresh tissue and frozen tissue examined immediately after thawing. Follicles at all stages of folliculogenesis up to the small antral stage survived freezing and thawing. Fresh and frozen tissue was transplanted underneath the kidney capsules of ovariectomized immunodeficient mice. The establishment of grafts was similar, and oestrogenic activity (cornification of the vaginal epithelium) was observed in the recipients 20 and 16 days after transplantation of fresh and frozen grafts respectively. The total number of follicles and the proportion of normal follicles were similar in fresh and frozen grafts. Grafts of frozen tissue recovered between 7 and 15 days after transfer contained follicles up to the small antral stage of development. Grafts recovered between 21 and 32 days contained follicles at all stages of folliculogenesis, including large antral follicles (1-2 mm diameter). Our results suggest that freezing and thawing do not substantially damage marmoset ovarian tissue, and the cryopreserved tissue retains its ability to support the development of large antral follicles.

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Orthotopic Ovarian Grafts in the Golden Hamster

Cited by 16 publications

References 3 publications

Offspring produced from heterotopic ovarian allografts in male and female recipient mice

Offspring produced from heterotopic ovarian allografts in male and female recipient mice

Cryopreservation of vascularized ovary: An evaluation of histology and function in rats

Ovary and ovulation: Follicular development in cryopreserved marmoset ovarian tissue after transplantation

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