Numerous dental patients have maxillofacial defects, requiring improved methods for orthopedic replacement. To extend the life of facial epitheses, proper hygiene care is crucial, yet literature on this is scarce. Most publications focus on hygiene for removable dentures, but facial epitheses' distinct materials and fixing methods necessitate different care approaches. The study aimed to develop and assess the toxicological safety of hygiene products for facial epitheses. The proposed products include a spray and a foam, both containing various compounds such as an aqueous extract from pine wood, fermented licorice extract, 1,2-pentanediol, sodium cocoamphoacetate, and deionized water.
To evaluate the cytotoxicity of these hygiene products, photopolymer material samples for facial prostheses were produced and treated with the spray, foam, or both for 2 minutes. One control group received no treatment. The samples were layered with a primary cell culture from a human mucosal biopsy of the alveolar process of the mandible, cultured with DMEM/F12 growth medium supplemented with 10% embryonic calf serum and 1% penicillin-streptomycin. Cell viability was assessed after 48 hours using culture staining and a fluorescence microscope. A colorimetric CTT test was used to measure living cell metabolic activity attached to the samples. Results revealed the proposed hygiene products are not cytotoxic and can be safely used clinically.