Alcohol-free beers
(AFBs) brewed by cold-contact fermentation exhibit
a flavor reminiscent of wort which affects consumer acceptability.
The aims of this study were to identify the odor-active compounds
in AFB and elucidate the contribution of these to the overall aroma
and worty character of the beer. Using a sensomics approach, 27 odor-active
aroma compounds were identified and quantitated using gas chromatography–mass
spectrometry. The most odor-active compound was methional (boiled
potato-like aroma), followed by 3-methylbutanal (cocoa-like), (
E
)-β-damascenone (apple, jam-like), 5-ethyl-3-hydroxy-4-methyl-2(5
H
)-furanone (curry, spicy-like), and phenylacetaldehyde
(floral, honey-like). The important contribution of these flavor compounds
to the worty and honey aroma of AFB was determined by sensory assessment
of the recombinate in a beer-like matrix with omission tests. The
role of 5-ethyl-3-hydroxy-4-methyl-2(5
H
)-furanone
in AFB aroma was reported for the first time. The outcomes from this
study are of relevance for the brewing industry to design strategies
for the reduction of the wortiness of AFB.