Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction

Torbet, J.; Malbouyres, Marilyne; Builles, Nicolas; Justin, V.; Roulet, Muriel; Damour, O.; Oldberg, Åke; Ruggiero, Florence; Hulmes, David J.S.

doi:10.1016/j.biomaterials.2007.05.024

Cited by 173 publications

(144 citation statements)

References 51 publications

Supporting

Mentioning

141

Contrasting

Unclassified

Order By: Relevance

“…Fiber diameter appeared to be on the order of hundreds of nanometers and transparency of the scaffold still needs to be measured. Torbet et al achieved fibril alignment by using magnetic orientation to create a stromal like scaffold of orthogonal layers of oriented collagen type I fibers (42). Proteoglycans were added to improve the transparency of the matrix (35% decorin and 65% lumican, keratocan, and osteoglycin) and were found to reduce the size and variability of the collagen fibers.…”

Section: Scaffold-based Approaches: Materials Used For Tissue-engineementioning

confidence: 99%

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

et al. 2008

View full text Add to dashboard Cite

ABSTRACT:The field of corneal tissue engineering has made many strides in recent years. The challenges of engineering a biocompatible, mechanically stable, and optically transparent tissue are significant. To overcome these challenges, researchers have adopted two basic approaches: cell-based strategies for manipulating cells to create their own extracellular matrix, and scaffold-based strategies for providing strong and transparent matrices upon which to grow cells. Both strategies have met with some degree of success. In addition, recent advances have been made in innervating a tissueengineered construct. Future work will need to focus on further improving mechanical stability of engineered constructs as well as improving the host response to implantation.

show abstract

Section: Scaffold-based Approaches: Materials Used For Tissue-engineementioning

confidence: 99%

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Previous studies have demonstrated the potential of collagenous scaffolds [8] and hydrogels [9] for tissue engineering applications [10]. The ability to assemble collagenous scaffolds in vitro with the same structure and properties as natural collagenous tissue would be of significant importance for studying cell-matrix interactions as well as for developing compatible engineered tissues.…”

Section: Introductionmentioning

confidence: 99%

Electromechanical properties of dried tendon and isoelectrically focused collagen hydrogels

et al. 2012

View full text Add to dashboard Cite

Assembling artificial collagenous tissues with structural, functional, and mechanical properties, which mimic natural tissues, is of vital importance for many tissue engineering applications. While the electromechanical properties of collagen are thought to play a role in, e.g., bone formation and remodeling, this functional property has not been adequately addressed in engineered tissues. Here, the electromechanical properties of rat tail tendon are compared with those of dried iso-electrically focused collagen hydrogels using piezoresponse force microscopy in ambient conditions. In both the natural tissue and the engineered hydrogel, D-periodic, type I collagen fibrils are observed, which exhibit shear piezoelectricity. While both tissues also exhibit fibrils with parallel orientation, Fourier transform analysis has revealed that the degree of parallel alignment of the fibrils in the tendon is three times that of the dried hydrogel. The results obtained demonstrate that iso-electrically focused collagen has similar structural and electromechanical properties to that of tendon, which is relevant for tissue engineering applications.

show abstract

“…The central stroma occupies approximately 90% of the corneal volume and 70% of its dry weight and is composed of a dense and highly organized ECM of collagen fibrils and proteoglycans (19). The stroma is the major component of transparent corneal tissue and is mainly comprised of type I and V collagens existing in parallel-organized fibrils with a uniform diameter of around 30 nm (20) that are further arranged in lamellae.…”

Section: General Description On Collagen In Connective Tissuesmentioning

confidence: 99%

Collagen Tissue Engineering: Development of Novel Biomaterials and Applications

et al. 2008

View full text Add to dashboard Cite

Scientific investigations involving collagen have inspired tissue engineering and design of biomaterials since collagen fibrils and their networks primarily regulate and define most tissues. The collagen networks form a highly organized, three-dimensional architecture to entrap other ingredients. Biomaterials are expected to function as cell scaffolds to replace native collagen-based extracellular matrix. The composition and properties of biomaterials used as scaffold for tissue engineering significantly affect the regeneration of neo-tissues and influence the conditions of collagen engineering. The complex scenario of collagen characteristics, types, fibril arrangement, and collagen structure-related functions (in a variety of connective tissues including bone, cartilage, tendon, skin and cornea) are addressed in this review. Discussion will focus on nanofibrillar assemblies and artificial synthetic peptides that mimic either the fibrillar structure or the elemental components of type I collagen as illustrated by their preliminary applications in tissue engineering. Conventional biomaterials used as scaffolds in engineering collagencontaining tissues are also discussed. The design of novel biomaterials and application of conventional biomaterials will facilitate development of additional novel tissue engineering bioproducts by refining the currently available techniques. The field of tissue engineering will ultimately be advanced by increasing control of collagen in native tissue and by continual manipulation of biomaterials. GENERAL DESCRIPTION ON COLLAGEN IN CONNECTIVE TISSUESThe extracellular matrix (ECM), provides physical support to tissues by occupying the intercellular space, acting not only as benign native scaffolding for arranging cells within connective tissues, but also as a dynamic, mobile, and flexible substance defining cellular behaviors and tissue function (1). For most soft and hard connective tissues (bone, cartilage, tendon, cornea, blood vessels, and skin) collagen fibrils and their networks function as ECM, the highly organized, three-dimensional (3D) architecture surrounding various cells. Collagen plays a dominant role in maintaining the biologic and structural integrity of ECM and is highly dynamic, undergoing constant remodeling for proper physiologic functions (1). Hence, the ideal goal of tissue regeneration is to restore both the structural integrity and the vivid remodeling process of native ECM, especially restoring the delicate collagen networks under which normal physiologic regeneration occurs.Collagen molecules have a triple-helical structure and the presence of 4-hydroxyproline resulting from a posttranslational modification of peptide-bound prolyl residues provides a distinctive marker of these molecules (2). To date, 28 collagen types have been identified; I, II, III, and V are the main types that make up the essential part of collagen in bone, cartilage, tendon, skin, and muscle. They also exist in fibrillar forms with elaborate 3D arrays in ECM (3-5).Bone tissu...

show abstract

Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction

Cited by 173 publications

References 51 publications

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

Electromechanical properties of dried tendon and isoelectrically focused collagen hydrogels

Collagen Tissue Engineering: Development of Novel Biomaterials and Applications

Contact Info

Product

Resources

About