Orthogonal dual molecularly imprinted polymer-based plasmonic immunosandwich assay: A double characteristic recognition strategy for specific detection of glycoproteins

Zhou, Licheng; Wang, Yijia; Xing, Rongrong; Chen, Jin; Liu, Jia; Li, Wei; Liu, Zhen

doi:10.1016/j.bios.2019.111729

Cited by 63 publications

(45 citation statements)

References 60 publications

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“…Zhou et al used CEA as a target to imprint MIPs [126]. CEA is a glycoprotein serving as a biomarker for colorectal, breast, ovarian, lung, gastric, bladder and pancreatic cancer [127,128].…”

Section: Mips and Cancermentioning

confidence: 99%

“…Orthogonal dual MIP-based plasmonic immunosandwich assay was used to measure the CEA levels in human serum for healthy and colon cancer patients. This method could distinguish between samples from colon cancer patients and healthy individuals [126]. Xing et al used a similar method to detect neuron-specific enolase in human serum, which is used as a protein biomarker of small cell lung cancer.…”

Section: Mips and Cancermentioning

confidence: 99%

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Molecularly Imprinted Polymers in Biological Applications

et al. 2020

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Molecularly imprinted polymers (MIPs) are currently widely used and further developed for biological applications. The MIP synthesis procedure is a key process, and a wide variety of protocols exist. The templates that are used for imprinting vary from the smallest glycosylated glycan structures or even amino acids to whole proteins or bacteria. The low cost, quick preparation, stability and reproducibility have been highlighted as advantages of MIPs. The biological applications utilizing MIPs discussed here include enzyme-linked assays, sensors, in vivo applications, drug delivery, cancer diagnostics and more. Indeed, there are numerous examples of how MIPs can be used as recognition elements similar to natural antibodies.

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Section: Mips and Cancermentioning

confidence: 99%

Section: Mips and Cancermentioning

confidence: 99%

Molecularly Imprinted Polymers in Biological Applications

et al. 2020

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“…Schematic illustration of (A) the duMIP‐PISA for the detection of protein biomarkers; [ 51 ] (B) odMIP‐PISA approach for detection of trace target glycoprotein; [ 52 ] (C) odA‐based sandwich immunoassay for detection of target glycoprotein; [ 53 ] (D) PASA approach for rapid and ultrasensitive probing of trace miRNAs [ 54 ] …”

Section: Analysis Techniquesmentioning

confidence: 99%

“…We have successfully quantitatively analyzed a variety of important biomarkers including alpha fetal protein (AFP), ALP, neuron specific enolase (NSE), carcinoma embryonic antigen (CEA) in serum and EPO in urine. [ 46,48‐50,52 ] These assays have been fully validated to fulfill clinical requirements in specificity and sensitivity. Particularly, the PISA have also been demonstrated to probe target proteins in tumor‐bearing mice, providing a potent tool for in‐situ detection in vivo .…”

Section: Applicationsmentioning

confidence: 99%

New Promises of Advanced Molecular Recognition: Bioassays, Single Cell Analysis, Cancer Therapy, and Beyond

Liu

2022

Chin. J. Chem.

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Comprehensive Summary The development of biomimetic affinity materials holds great value for scientific research and practical applications. Starting from boronic acid chemistry, we developed a series of boronate affinity materials (BAMs) with desired binding properties in aspects of binding pH, affinity and selectivity. Based on BAMs, molecularly imprinted polymers (MIPs) and aptamers capable of targeting vital biomolecules were rationally designed and prepared. Using these affinity reagents as potent tools and combining with advanced analytical techniques particularly liquid chromatography, mass spectrometry and spectrometry allowed for the construction of promising analytical methods to provide new qualitative and quantitative solutions to challenging applications including targeted proteomics analysis, single cell analysis and disease diagnostics. In addition, the targeting capability of these affinity reagents particularly nanoscale MIPs opened a unique access to develop innovative nanotherapeutics for cancer treatment. What is the most favorite and original chemistry developed in your research group? So far, the most favorite and original chemistry developed by us is that HER2‐glycan‐imprinted nanoparticles could function as an anti‐cancer drug through blocking the HER2 signaling pathway and thereby inhibiting the growth of HER2+ breast cancer. How do you get into this specific field? Could you please share some experiences with our readers? Actually, I was not educated and trained to be a scientist for my current research field. During my graduate education and post‐doctoral research, separation science, particularly liquid chromatography and capillary electrophoresis, was the focus of my study and research. After I started my independent research career, I had to do something different from what I was trained and the contributions for which my previous supervisors were well recognized worldwide. I first picked up boronate affinity chromatography, a unique mode of affinity chromatography with great potentials but less explored, to start up. This choice was not because it was hot; rather, because there were many challenges that hamper this technique to be practically useful. Along with solving these issues one by one, I recognized that we need not to go elsewhere since this field is so cool that we can do quite a lot of things different from others. What is the most important personality for scientific research? I believe that persistence is the most important personality for scientific research, because it is the key to success. Albert Einstein once said, “It's not that I'm so smart, it's just that I stay with problems longer.” If you are not smarter than Einstein, think about the chances for you to succeed if you do not persist. What are your hobbies? I love walking, hiking and listening to music. Waling and hiking make me healthy while listening to music drives me into diverse favorite moods, be deep in thought, be passionate, and so on. I am good at whistling. I like to mimick bird singing walking in f...

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“…To solve the problems of low abundance of PMGs and the abundant interfering biomolecules, enrichment of glycosylated molecules is necessary before identification and analysis. There are several methods for enrichment of glycosylated molecules: hydrazide chemistry, 14 lectin affinity chromatography, 15 the boric acid affinity technique, [16][17][18][19] and hydrophilic interaction liquid chromatography (HILIC). 20 Among them, HILIC is one of the most important methods for the enrichment of glycopeptides and has received widespread attention due to its high enrichment performance and simple operation.…”

Section: Introductionmentioning

confidence: 99%