Orthogonal Activation of RNA‐Cleaving DNAzymes in Live Cells by Reactive Oxygen Species

Xiao, Lü; Gu, Chunmei; Xiang, Yu

doi:10.1002/ange.201908105

Cited by 17 publications

(1 citation statement)

References 66 publications

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“…Further by combination of the caged DNAzymes with upconversion nanoparticles (UCNPs), they realized the activation of DNAzyme activity in an NIR region [44] . In 2019, the Xiang Lab constructed an reactive oxygen species (ROS) activated DNAzyme by incorporation PS or phenylboronate groups at specific backbone positions in 8–17 DNAzyme by the efficient chemical conjugation reaction [45] . The Shionoya group demonstrated the concept of sharp switching of DNAzyme activity by introducing a Cu II ‐mediated carboxyimidazole base pair (ImC−Cu II −ImC) in the splitted catalytic core of E5 DNAzyme [46] .…”

Section: Expanding Of Dnazyme Librarymentioning

confidence: 99%

Recent Advances on DNAzyme‐Based Sensing

Huang

Wang

et al. 2022

Chemistry An Asian Journal

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Owing to the high sensitivity, excellent programmability, and flexible obtainment through in vitro selection, RNA‐cleaving DNAzymes have attracted increasing interest in developing DNAzyme‐based sensors. In this review, we summarize the recent advances on DNAzyme‐based sensing applications. We initially conclude two general strategies to expand the library of DNAzmes, in vitro selection to discover new DNAzymes towards different targets of interest and chemical modifications to endue the existing DNAzymes with new function or properties. We then discuss the recent applications of DNAzyme‐based sensors for the detection of a variety of important biomoleucles both in vitro and in vivo. Finally, perspectives on the challenges and future directions in the development of DNAzyme‐based sensors are provided.

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Section: Expanding Of Dnazyme Librarymentioning

confidence: 99%

Recent Advances on DNAzyme‐Based Sensing

Huang

Wang

et al. 2022

Chemistry An Asian Journal

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Enzyme‐Mediated Endogenous and Bioorthogonal Control of a DNAzyme Fluorescent Sensor for Imaging Metal Ions in Living Cells

et al. 2019

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Bioorthogonal control of metal‐ion sensors for imaging metal ions in living cells is important for understanding the distribution and fluctuation of metal ions. Reported here is the endogenous and bioorthogonal activation of a DNAzyme fluorescent sensor containing an 18‐base pair recognition site of a homing endonuclease (I‐SceI), which is found by chance only once in 7×1010 bp of genomic sequences, and can thus form a near bioorthogonal pair with I‐SceI for DNAzyme activation with minimal effect on living cells. Once I‐SceI is expressed inside cells, it cleaves at the recognition site, allowing the DNAzyme to adopt its active conformation. The activated DNAzyme sensor is then able to specifically catalyze cleavage of a substrate strand in the presence of Mg2+ to release the fluorophore‐labeled DNA fragment and produce a fluorescent turn‐on signal for Mg2+. Thus I‐SceI bioorthogonally activates the 10–23 DNAzyme for imaging of Mg2+ in HeLa cells.

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Target Self‐Enhanced Selectivity in Metal‐Specific DNAzymes

et al. 2020

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Highly selective recognition of metal ions by rational ligand design is challenging, and simple metal binding by biological ligands is often obscured by nonspecific interactions. In this work, binding‐triggered catalysis is used and metal selectivity is greatly increased by increasing the number of metal ions involved, as exemplified in a series of in vitro selected RNA‐cleaving DNAzymes. The cleavage junction is modified with a glycyl–histidine‐functionalized tertiary amine moiety to provide multiple potential metal coordination sites. DNAzymes that bind 1, 2, and 3 Zn2+ ions, increased their selectivity for Zn2+ over Co2+ ions from approximately 20‐, 1000‐, to 5000‐fold, respectively. This study offers important insights into metal recognition by combining rational ligand design and combinatorial selection, and it provides a set of new DNAzymes with excellent selectivity for Zn2+ ions.

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Orthogonal Activation of RNA‐Cleaving DNAzymes in Live Cells by Reactive Oxygen Species

Cited by 17 publications

References 66 publications

Recent Advances on DNAzyme‐Based Sensing

Recent Advances on DNAzyme‐Based Sensing

Enzyme‐Mediated Endogenous and Bioorthogonal Control of a DNAzyme Fluorescent Sensor for Imaging Metal Ions in Living Cells

Target Self‐Enhanced Selectivity in Metal‐Specific DNAzymes

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