The purpose of the study was to adapt the 21st Century Skills Teaching Scale into Turkish Culture. The research was conducted with 233 prospective teachers in the Fall Term of 2019-2020 Academic Year. The scale serves the purpose of measuring prospective teachers' self-efficacy perceptions regarding the teaching of 21 st century skills and consists of 10 items and three factors. Sub-factors of the scale are utility of technology, collaboration and innovation & problem solving. In the adaptation process of the scale to Turkish, linguistic validity, conceptual and experiential equivalence, construct validity, criterion validity and reliability studies were carried out. In order to ensure the linguistic validity of the Turkish form of the scale, translation back translation technique was applied and expert opinions were asked. Confirmatory Factor Analysis (CFA) was applied to test the construct validity. To test the criterion validity, the correlation between the whole scale and each sub-factor was examined. Within the scope of reliability studies, Cronbach Alfa (internal consistency) reliability coefficients of the scale, halftest reliability, item total correlations and item discrimination values were calculated. As a result of the research, it has been determined that the 21 st Century Skills Teaching Scale Turkish Form is a valid and reliable measurement tool that can be used to measure the self-efficacy perceptions of prospective teachers regarding 21 st century skills teaching.