Orphan nuclear receptor<i>ftz-f1 (NR5A3)</i>promotes egg chamber survival in the<i>Drosophila</i>ovary

Beachum, Allison N.; Whitehead, Kaitlin M.; McDonald, Samantha I; Phipps, Daniel N.; Berghout, Hanna E; Ables, Elizabeth T.

doi:10.1093/g3journal/jkab003

Cited by 8 publications

(7 citation statements)

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“…The expression of MnFtz-f1 in the CS of M. nipponense was significantly higher than that in the other developmental stages (Figure 7); this showed that MnFtz-f1 might play an important role in the process of oocyte mitosis. A recent study in Drosophila revealed that Drosophila oocytes could not undergo normal mitosis in the absence of Ftz-f1, suggesting that Ftz-f1 was essential for oocyte division (57). In Drosophila, Ftz-f1 is divided into two subtypes: aFtz-f1 and bFtz-f1.…”

Section: Discussionmentioning

confidence: 99%

MnFtz-f1 Is Required for Molting and Ovulation of the Oriental River Prawn Macrobrachium nipponense

Yuan

Zhang

et al. 2021

Front. Endocrinol.

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Molting and ovulation are the basic processes responsible for the growth and reproduction of Macrobrachium nipponense; however, the molecular mechanisms of molting and ovulation in M. nipponense are poorly understood. The present study aimed to use MnFtz-f1 as the starting point to study the molting and ovulation phenomena in M. nipponense at the molecular level. The full-length MnFtz-f1 cDNA sequence was 2,198 base pairs (bp) in length with an open reading frame of 1,899 bp encoding 632 amino acids. Quantitative real-time PCR analysis showed that MnFtz-f1 was highly expressed in the ovary at the cleavage stage and on the fifth day after hatching. In vivo administration of 20-hydroxyecdysone (20E) showed that 20E effectively inhibited the expression of the MnFtz-f1 gene, and the silencing of the MnFtz-f1 gene reduced the content of 20E in the ovary. In situ hybridization (ISH) analysis revealed the localization of MnFtz-f1 in the ovary. Silencing of MnFtz-f1 by RNA interference (RNAi) resulted in significant inhibition of the expression of the vitellogenin (Vg), Spook, and Phantom genes, thus confirming that MnFtz-f1 had a mutual regulatory relationship with Vg, Spook, and Phantom. After RNAi, the molting frequency and ovulation number of M. nipponense decreased significantly, which demonstrated that MnFtz-f1 played a pivotal role in the process of molting and ovulation.

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Section: Discussionmentioning

confidence: 99%

MnFtz-f1 Is Required for Molting and Ovulation of the Oriental River Prawn Macrobrachium nipponense

Yuan

Zhang

et al. 2021

Front. Endocrinol.

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“…BgFtz-F1 is expressed throughout the ovary during the whole gonadotrophic cycle, and its depletion results in the complete block of oogenesis due, at least in part, to failure in the reorganization of the actin filaments of the follicle cells during choriogenesis. Interestingly, FTZ-F1 is also required for completing oogenesis in T. castaneum (Xu et al, 2010) and D. melanogaster (Beachum et al, 2021; Knapp et al, 2020). In the fly, like in B. germanica , this factor is detected throughout the ovary, and is required for follicle cells to differentiate properly into the final maturation stage (Beachum et al, 2021; Knapp et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Section: Bgftz-f1 Likely Controls Embryo Segmentation and Is Required...mentioning

confidence: 99%

“…The copyright holder for this preprint this version posted March 12, 2022. ; https://doi.org/10.1101/2022.03.10.483750 doi: bioRxiv preprint completing oogenesis in T. castaneum (Xu et al, 2010) and D. melanogaster (Beachum et al, 2021;Knapp et al, 2020). In the fly, like in B. germanica, this factor is detected throughout the ovary, and is required for follicle cells to differentiate properly into the final maturation stage (Beachum et al, 2021;Knapp et al, 2020). Likewise, the mammalian FTZ-F1 homolog, steroidogenic factor-1 (SF-1) has been also detected in somatic follicle cells in rodents and humans (Hinshelwood et al, 2003;Tajima et al, 2003), and its depletion in mouse granulosa cells results in the loss of developing follicles (Pelusi et al, 2008).…”

Section: Bgftz-f1 Likely Controls Embryo Segmentation and Is Required...mentioning

confidence: 99%

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Ecdysone signaling controls early embryogenesis in the short-germ hemimetabolous insect Blattella germanica

Cruz

Maestro

Franch-Marro

2022

Preprint

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Steroid hormone signaling is a central regulator of insect development especially during the post-embryonic period. However, its role during embryonic development is less understood, particularly in short-germ band hemimetabolous insects. Here, we used the short-germ band hemimetabolous insect Blattella germanica to analyse the functions of 20-hydroxyecdysone (20E), the most active insect steroid, signaling during the embryonic stage. We show that the heterodimeric 20E receptor, BgEcR-A and BgRXR, is present from the beginning of embryogenesis and that a stereotypical 20E-dependent cascade of nuclear receptors is first detected at the early blastoderm stage. Using parental RNAi, we show that both receptors are required for the proper formation of the germ band by controlling cell proliferation during the blastoderm stage. In addition, they are also required for the coordinated induction of the 20E-induced cascade of nuclear receptors. Finally, we show that two of these nuclear receptors, BgHR3 and BgFTZ-F1, have relevant roles in germ band formation. Whereas BgHR3 controls the formation and development of the cephalic region of the germ band, BgFTZ-F1 stripped expression suggested a role in the segmentation of the germ band. In summary, our results show that 20E-signaling is required much earlier in short-germ band hemimetabolous than in long-germ band holometabolous embryogenesis, which raises the possibility that the loss of 20E-signaling during the initial stages of embryogenesis could be a key feature in the evolution of the different types of insect development.

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“…In recent years, Drosophila has served as a classic in vivo model for use in studying aging and reproduction (23), since Drosophila reproductive biology resembles that of mammals (24,25). As compared to other animal models, Drosophila has a simple ovarian structure, short reproductive cycle, and easy gene editing (26).…”

Section: Introductionmentioning

confidence: 99%

Ginsenosides improve reproductive capability of aged female Drosophila through mechanism dependent on ecdysteroid receptor (ECR) and steroid signaling pathway

Liu

et al. 2022

Front. Endocrinol.

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Aging ovaries caused diminished fertility and depleted steroid hormone level. Ginsenosides, the active ingredient in ginseng, had estrogen-like hormonal effects. Although ginsenosides were well known for their ability to alleviate many age-related degenerative diseases, the effect of ginsenosides on the decline in reproductive capability caused by aging, as well as the mechanism, are unknown. We found that ginsenosides improved the quantity and quality of the offspring, prolonged life and restored muscle ability in aged female Drosophila. In addition, ginsenosides inhibited ovarian atrophy and maintained steroid hormone 20-Hydroxyecdysone (20E) and juvenile-preserving hormone (JH)) levels. Ginsenosides activated ecdysteroid receptor (ECR) and increased the expression of the early transcription genes E74 and Broad (Br), which triggered steroid signaling pathway. Meanwhile, ginsenosides promoted JH biosynthesis by increasing the expression of Hydroxyl-methylglutaryl-CoA reductase (HMGR) and juvenile hormone acid O-methyltransferase (JHAMT). Subsequently, JH was bound to Methoprene Tolerant (Met) and activated the transcription of the responsive gene Kruppel Homolog 1 (Kr-h1), which coordinated with 20E signaling to promote the reproduction of aged female Drosophila. The reproductive capacity and steroid hormone levels were not improved and the steroid signaling pathway was not activated in ginsenoside-treated ECR knockout Drosophila. This suggested that ginsenosides played a role dependent on targeted ECR. Furthermore, 17 kinds of ginsenoside monomers were identified from the total ginsenosides. Among them, Rg1, Re and Rb1 improved the reproductive capacity and steroid hormone levels of aged female Drosophila, which has similar effects to the total ginsenoside. These results indicated that ginsenosides could enhance the reproductive capacity of aged female Drosophila by activating steroid signals dependent on nuclear receptor ECR. In addition, ginsenoside monomers Rg1, Rb1 and Re are the main active components of total ginsenosides to improve reproductive ability. This will provide strong evidence that ginsenosides had the potential to alleviate age-induced reproductive degradation.

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Orphan nuclear receptorftz-f1 (NR5A3)promotes egg chamber survival in theDrosophilaovary

Cited by 8 publications

References 100 publications

MnFtz-f1 Is Required for Molting and Ovulation of the Oriental River Prawn Macrobrachium nipponense

MnFtz-f1 Is Required for Molting and Ovulation of the Oriental River Prawn Macrobrachium nipponense

Ecdysone signaling controls early embryogenesis in the short-germ hemimetabolous insect Blattella germanica

Ginsenosides improve reproductive capability of aged female Drosophila through mechanism dependent on ecdysteroid receptor (ECR) and steroid signaling pathway

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