Oroxylum indicum root bark extract prevents doxorubicin-induced cardiac damage by restoring redox balance

Menon, Seema; Lawrence, Lincy; Sivaram, V.; Padikkala, José

doi:10.1016/j.jaim.2017.06.007

Cited by 22 publications

(9 citation statements)

References 35 publications

(36 reference statements)

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“…ese results were also in line with the present study. Not only these studies but also many other previous studies were consistent with the results of the present study [16,24,54]. In the present study, doxorubicin-treated rats showed large areas with early changes of necrosis and reversible histological changes including inflammatory infiltrations, interstitial oedema, haemmorrhage, congestion of blood vessels, wavy myocardial fibers, and intracellular vacuoles.…”

Section: Evidence-based Complementary and Alternative Medicinesupporting

confidence: 93%

“…It is widely reported that Murraya leaf extract is a rich source of phenolic compounds and alkaloids (carbazole alkaloids) which contribute to the antioxidant activity by scavenging free radicals effectively [8,9,15]. As the major mechanism of doxorubicin-induced cardiotoxicity is oxidative stress which is caused by an imbalance between intracellular antioxidant system and the ROS production during doxorubicin metabolism, it is believed that enrichment of heart muscles with antioxidants may be important to prevent cardiotoxicity induced by doxorubicin [16]. erefore, supplementation therapies with high amounts of antioxidants have been identified as effective measures to prevent doxorubicin-induced cardiotoxicity [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Cardioprotective Potential of Murraya koenigii (L.) Spreng. Leaf Extract against Doxorubicin‐Induced Cardiotoxicity in Rats

Sandamali

Hewawasam

Jayatilaka

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Dose-dependent cardiotoxicity of doxorubicin may lead to irreversible congestive heart failure. Although multiple mechanisms are involved, generation of free radicals is the most commonly postulated mechanism. erefore, free radical scavengers are considered as potential therapeutic agents. As Murraya koenigii leaves are a rich source of flavonoids and phenols, they have the ability to scavenge free radicals effectively. erefore, the objective of this study was to investigate the cardioprotective potential of Murraya leaf extract against doxorubicin-induced cardiotoxicity in rats. Rats were randomly divided into five groups with 10 animals in each group. Doxorubicin was administered intraperitonially at 18 mg/kg while lyophilized plant extract was administered orally at 2 g/kg. Dexrazoxane, at 180 mg/kg, was used as the positive control. Cardiac damage of doxorubicin control was evident with a significant increase (p < 0.05) in cardiac troponin I, NT-pro BNP, AST, and LDH compared to the normal control. Plant-treated group showed cardioprotective effect by significantly reducing (p < 0.05) all of the above parameters compared to doxorubicin control (p < 0.05). Increased oxidative stress in doxorubicin control was evident with a significant reduction in reduced glutathione, glutathione reductase, glutathione peroxidase, total antioxidant capacity, superoxide dismutase, and catalase activity and a significant increase in lipid peroxidation compared to the control. Interestingly, treatment with Murraya leaf extract showed a significant increase in all of the above antioxidant parameters and a significant reduction in lipid peroxidation by showing an antioxidant effect. A significant increase in myeloperoxidase activity confirmed the increased inflammatory activity in doxorubicin control group whereas plant-treated group showed a significant reduction (p < 0.05) which expressed the anti-inflammatory effect of Murraya leaf extract. Doxorubicin-treated group showed histological evidence of extensive damage to the myocardium while plant-treated group showed a preserved myocardium with lesser degree of damage. Pretreatment with Murraya leaf extract may replenish cardiomyocytes with antioxidants and promote the defense against doxorubicin-induced cardiotoxicity.

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Section: Evidence-based Complementary and Alternative Medicinesupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Cardioprotective Potential of Murraya koenigii (L.) Spreng. Leaf Extract against Doxorubicin‐Induced Cardiotoxicity in Rats

Sandamali

Hewawasam

Jayatilaka

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Due to enlargement of the sarcoplasmic reticulum, DOX induced several degenerative changes in heart tissue, including myocardial hyalinization, and sarcoplasmic vacuolization [ 107 ], wavy myocardial fibers flocculation, and fragmentation. In addition, multifocal areas of myocardial necrosis, myofibrillar loss, inflammatory cell infiltrations, myocardial fibrosis, hemorrhage, vascular congestion, and interfibrillar edema were observed [ 71 , 74 , 100 , 108 , 109 ]. On the contrary, cardiac tissues of DPPE plus DOX-treated animals showed a marked improvement in cardiac tissue structure and integrity.…”

Section: Discussionmentioning

confidence: 99%

Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways

Elblehi

El‐Sayed

Soliman

et al. 2021

Animals

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Doxorubicin (DOX) has a potent antineoplastic efficacy and is considered a cornerstone of chemotherapy. However, it causes several dose-dependent cardiotoxic results, which has substantially restricted its clinical application. This study was intended to explore the potential ameliorative effect of date palm pollen ethanolic extract (DPPE) against DOX-induced cardiotoxicity and the mechanisms underlying it. Forty male Wistar albino rats were equally allocated into Control (CTR), DPPE (500 mg/kg bw for 4 weeks), DOX (2.5 mg/kg bw, intraperitoneally six times over 2 weeks), and DPPE + DOX-treated groups. Pre-coadministration of DPPE with DOX partially ameliorated DOX-induced cardiotoxicity as DPPE improved DOX-induced body and heart weight changes and mitigated the elevated cardiac injury markers activities of serum aminotransferases, lactate dehydrogenase, creatine kinase, and creatine kinase-cardiac type isoenzyme. Additionally, the concentration of serum cardiac troponin I (cTnI), troponin T (cTnT), N-terminal pro-brain natriuretic peptide (NT-pro BNP), and cytosolic calcium (Ca+2) were amplified. DPPE also alleviated nitrosative status (nitric oxide) in DOX-treated animals, lipid peroxidation and antioxidant molecules as glutathione content, and glutathione peroxidase, catalase, and superoxide dismutase activities and inflammatory markers levels; NF-κB p65, TNF-α, IL-1β, and IL-6. As well, it ameliorated the severity of histopathological lesions , histomorphometric alteration and improved the immune-staining of the pro-fibrotic (TGF-β1), pro-apoptotic (caspase-3 and Bax), and anti-apoptotic (Bcl-2) proteins in cardiac tissues. Collectively, pre-coadministration of DPPE partially mitigated DOX-induced cardiac injuries via its antioxidant, anti-inflammatory, anti-fibrotic, and anti-apoptotic potential.

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“…Fruit pods of this plant have been used in many Asian countries as a plantbased food and herbal medicine for thousands of years without any known adverse effects [7][8][9]. Numerous scientific studies showed that O. indicum was not toxic even in experimental animals, even up to high doses [10][11][12]. e fruit pods of this plant are rich in nutrients concerning dietary fiber, essential amino acids, minerals, and fatty acids [8].…”

Section: Introductionmentioning

confidence: 99%

Intracellular ROS Scavenging and Anti‐Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN‐γ‐Activated RAW264.7 Macrophages

Dunkhunthod

Talabnin

Murphy

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Oroxylum indicum (L.) Kurz has been used as plant-based food and herbal medicine in many Asian countries. The aim of the present study was to examine the antioxidant and anti-inflammatory activities of O. indicum extract (O. indicum) in RAW264.7 cells activated by LPS plus IFN-γ. The phytochemical compounds in O. indicum were identified by GC-MS and LC-MS/MS. Five flavonoids (luteolin, apigenin, baicalein, oroxylin A, and quercetin) and 27 volatile compounds were found in O. indicum. O. indicum presented antioxidant activities, including reducing ability by FRAP assay and free radical scavenging activity by DPPH assay. Moreover, O. indicum also suppressed LPS plus IFN-γ-activated reactive oxygen species generation in RAW264.7 macrophages. It possessed the potent anti-inflammatory action through suppressing nitric oxide (NO) and IL-6 secretion, possibly due to its ability to scavenge intracellular ROS. The synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy results showed the alteration of signal intensity and integrated areas relating to lipid and protein of the activated RAW264.7 macrophages compared to unactivated cells. This is the first report of an application of the SR-FTIR technique to evaluate biomolecular changes in activated RAW264.7 cells. Our results indicate that O. indicum may be used as a potential source of nutraceutical for the development of health food supplement or a novel anti-inflammatory herbal medicine.

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Oroxylum indicum root bark extract prevents doxorubicin-induced cardiac damage by restoring redox balance

Abstract: O. indicum Vent. root bark shows marked cardio-protective activity, possibly due to the presence of antioxidant compounds acting synergistically.

Cited by 22 publications

References 35 publications

Cardioprotective Potential of Murraya koenigii (L.) Spreng. Leaf Extract against Doxorubicin‐Induced Cardiotoxicity in Rats

Cardioprotective Potential of Murraya koenigii (L.) Spreng. Leaf Extract against Doxorubicin‐Induced Cardiotoxicity in Rats

Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways

Intracellular ROS Scavenging and Anti‐Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN‐γ‐Activated RAW264.7 Macrophages

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