Orosomucoid secretion levels by epicardial adipose tissue as possible indicator of endothelial dysfunction in diabetes mellitus or inflammation in coronary artery disease

Fandiño-Vaquero, Rubén; Fernández-Trasancos, Ángel; Álvarez, Ezequiel; Ahmad, Samah; Batista-Oliveira, A.L.; Adrio, Belén; Fernández, Ángel L.; González-Juanatey, José Ramón; Eiras, Sonia

doi:10.1016/j.atherosclerosis.2014.05.921

Cited by 31 publications

(26 citation statements)

References 33 publications

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“…Proinflammatory cytokines IL‐6 and TNF have a close relationship with the occurrence and development of atherosclerosis and CHD . In patients with CHD, proinflammatory cytokines induced by perioperative stimuli may be one of the significant causes of coronary spasm, plaque rupture or haemorrhage, leading to unstable angina or myocardial infarction . In the present study, we found that IL‐6 concentrations increased at the end of surgery, whereas TNF‐ α concentrations did not change in either group and dexmedetomidine minimized the increase in IL‐6.…”

Section: Discussionsupporting

confidence: 45%

Does dexmedetomidine have a cardiac protective effect during non‐cardiac surgery? A randomised controlled trial

Shen

et al. 2014

Clin Exp Pharma Physio

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This study was designed to determine the effects of dexmedetomidine on perioperative myocardial injury by observing peripheral circulatory changes in response to tracheal intubation and extubation, myocardial enzyme levels, myocardial ischaemia improvements, cardiovascular adverse events and cytokines in patients with coronary heart disease (CHD) undergoing non-cardiac surgery. This study was a prospective, randomized, double-blind trial. Eighty patients having CHD were scheduled for elective hip-replacement surgery and randomly allocated to receive a loading dose of 1 μg/kg dexmedetomidine followed by a 0.2 μg/kg per h infusion (Dex group; n = 40) or normal saline (control group; n = 40). Systolic blood pressure, diastolic blood pressure, mean arterial pressure, heart rate, rate-pressure product and changes in ST-T segment on the electrocardiogram were recorded every 5 min during surgery. Serum creatine kinase-MB (CK-MB), cardiac troponin I (cTnI), glycogen phosphorylase BB (GP-BB), interleukin (IL)-6 and tumour necrosis factor (TNF)-α protein levels were determined preoperatively, at the end of surgery and 12 and 24 h after surgery. The improvement rate of myocardial ischaemia was higher in the Dex than control group (87.5% vs 32.5%, respectively; P < 0.05). In addition, the Dex group had lower serum CK-MB, IL-6, cTnI and GP-BB concentrations than the control group (P < 0.05). There was no significance difference in TNF-α between the two groups (P > 0.05). Dexmedetomidine can reduce myocardial injury and cytokine levels in patients with CHD undergoing non-cardiac surgery.

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Section: Discussionsupporting

confidence: 45%

Does dexmedetomidine have a cardiac protective effect during non‐cardiac surgery? A randomised controlled trial

Shen

et al. 2014

Clin Exp Pharma Physio

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“…The real-time cell analyser (RTCA SP Instrument; Roche Applied Science) is an impedance-based technology that was used for label-free and real-time monitoring of the cell viability of HUVEC under different conditions (Fandino-Vaquero et al 2014). Viability and growth of HUVEC in the presence of HSA, ct-HSA, gHSA or AGE-HSA at different concentrations (12-400 mg/ml) in EBM-2 medium were registered continuously during 12 h. The system monitored cellular events in real time measuring electrical impedance across interdigitated micro-electrodes integrated on the bottom of cell culture E-plates.…”

Section: Cell Culture and Cell Viability's Experimentsmentioning

confidence: 99%

Key structural and functional differences between early and advanced glycation products

Paradela-Dobarro

Rodiño-Janeiro

Alonso

et al. 2016

Journal of Molecular Endocrinology

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Most of the studies on advanced glycation end products (AGE) have been carried out with uncharacterized mixtures of AGE, so the observed effects cannot be linked to defined structures. Therefore, we analysed the structural differences between glycated human serum albumin (gHSA), a low glycated protein, and AGE-human serum albumin (AGE-HSA), a high glycated protein, and we compared their effects on endothelial functionality. Specifically, we characterized glycation and composition on both early and advanced stage glycation products of gHSA and AGE-HSA by using the MALDI-TOF-mass spectrometry assay. Furthermore, we studied the effects of both types of glycation products on reactive oxygen species (ROS) production and in the expression of vascular and intercellular cell adhesion molecules (VCAM-1 and ICAM-1) on human umbilical endothelial cells (HUVEC). We also measured the adhesion of peripheral blood mononuclear cells (PBMC) to HUVEC. Low concentrations of gHSA enhanced long-lasting ROS production in HUVEC, whereas lower concentrations of AGE-HSA caused the anticipation of the induced extracellular ROS production. Both gHSA and AGE-HSA up-regulated the expression of VCAM-1 and ICAM-1 at mRNA levels. Nevertheless, only AGE-HSA increased protein levels and enhanced the adhesion of PBMC to HUVEC monolayers. Functional differences were observed between gHSA and AGE-HSA, causing the latter an anticipation of the pro-oxidant effects in comparison to gHSA. Moreover, although both molecules induced genetic up-regulation of adhesion molecules in HUVEC, only the high glycated protein functionally increased mononuclear cell adhesion to endothelial monolayers. These observations could have important clinical consequences in the development of diabetic vascular complications.

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“…Although it is mainly synthesized by the liver, many extrahepatic tissues, including adipocytes, heart, and brain, are capable of producing ORM under myriad physiological and pathological conditions (16)(17)(18)(19). A variety of activities have been attributed to ORM, which include modulating immunity, carrying drugs, maintaining the capillary barrier, and mediating sphingolipid metabolism (14,(20)(21)(22)(23). It has been reported that the effects of ORM on macrophages, neutrophils, and liver parenchymal cells are mediated by membrane receptor CCR5, Siglect-5, and hemoglobin b-chain, respectively (24)(25)(26).…”

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confidence: 99%

The Acute-Phase Protein Orosomucoid Regulates Food Intake and Energy Homeostasis via Leptin Receptor Signaling Pathway

et al. 2016

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The acute-phase protein orosomucoid (ORM) exhibits a variety of activities in vitro and in vivo, notably modulation of immunity and transportation of drugs. We found in this study that mice lacking ORM1 displayed aberrant energy homeostasis characterized by increased body weight and fat mass. Further investigation found that ORM, predominantly ORM1, is significantly elevated in sera, liver, and adipose tissues from the mice with high-fat diet (HFD)-induced obesity and db/db mice that develop obesity spontaneously due to mutation in the leptin receptor (LepR). Intravenous or intraperitoneal administration of exogenous ORM decreased food intake in C57BL/6, HFD, and leptin-deficient ob/ob mice, which was absent in db/db mice and was significantly reduced in mice with arcuate nucleus (ARC) LepR knockdown, whereas enforced expression of ORM1 in ARC significantly decreased food intake, body weight, and serum insulin level. Furthermore, we found that ORM is able to bind directly to LepR and activate the receptor-mediated JAK2-STAT3 signaling in hypothalamus tissue and GT1-7 cells, which was derived from hypothalamic tumor. These data indicated that ORM could function through LepR to regulate food intake and energy homeostasis in response to nutrition status. Modulating the expression of ORM is a novel strategy for the management of obesity and related metabolic disorders.

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Orosomucoid secretion levels by epicardial adipose tissue as possible indicator of endothelial dysfunction in diabetes mellitus or inflammation in coronary artery disease

Cited by 31 publications

References 33 publications

Does dexmedetomidine have a cardiac protective effect during non‐cardiac surgery? A randomised controlled trial

Does dexmedetomidine have a cardiac protective effect during non‐cardiac surgery? A randomised controlled trial

Key structural and functional differences between early and advanced glycation products

The Acute-Phase Protein Orosomucoid Regulates Food Intake and Energy Homeostasis via Leptin Receptor Signaling Pathway

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