Ornithine decarboxylase and polyamines in silkmoth pupal tissues: Effects of ecdysone and injury

Wyatt, G.R.; Rothaus, K.; Lawler, D.; Herbst, Edward J.

doi:10.1016/0304-4165(73)90268-7

Cited by 53 publications

(22 citation statements)

References 29 publications

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“…ODCs, enzymes catalyzing polyamine production, were highly up-regulated in the spermatheca by both mating and 20E treatment. Interestingly, ODCs are hormonally controlled in insects (50), and both ODCs and polyamine synthesis have been linked to egg development and egg-laying in some species in which inhibition of ODC activity resulted in impaired vitellogenesis (51) and a delay in oviposition (46). 20E released from the mating plug therefore may induce egg-laying via the stimulation of polyamine synthesis by the sperm storage organ, which in turn may trigger yet unknown downstream cascades.…”

Section: Discussionmentioning

confidence: 99%

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Gabrieli

Kakani

Mitchell

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

109

174

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Significance Anopheles gambiae females are the principal vectors of malaria, a disease that kills more than 600,000 people every year. Current control methods using insecticides to kill mosquitoes are threatened by the spread of resistance in natural populations. A promising alternative control strategy is based on interfering with mosquito reproduction to reduce the number of malaria-transmitting females. Here we show that a male hormone transferred to the female during sex induces large changes in female behavior. These changes, defined as the postmating switch, include a physical incapacity for fertilization by additional males and the ability to lay mature eggs. Tampering with the function of this hormone generates unprecedented opportunities to reduce the reproductive success of Anopheles mosquitoes and impact malaria transmission.

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Section: Discussionmentioning

confidence: 99%

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Gabrieli

Kakani

Mitchell

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

109

174

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“…The enzymatic activity was determined by the method described by Wyatt et al (1973), modified by Cardoso et al (2002).…”

Section: Preparation Of Tissues and Odc Activity Assaysmentioning

confidence: 99%

Ornithine decarboxylase activity during the development of Anastrepha fraterculus (Diptera, Tephritidae)

Cardoso

Prestes

Casali

et al. 2004

Arch Insect Biochem Physiol

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Ornithine decarboxylase (ODC) (EC 4.1.1.17) is very important for polyamine biosynthesis, which is required for main biological events. In the present study, ODC activity was measured in samples of Anastrepha fraterculus's egg, larva, pupa body and abdomen, adult body, ovaries, and fat body of young females, and in ovaries of mature flies. The kinetic parameters (Km app and Vmax) for ODC activity were determined for pupa, larva, and young ovary. ODC activity showed fluctuations during A. fraterculus's life development. In its earlier stages, prior to emergence, the egg has high ODC-specific activity probably due to embryogenesis, which is characterized by a high rate of cell division. This enzyme activity is also significantly high in the ovary and fat body of young females possibly related to the increased oogenesis and vitellogenesis. The kinetic parameters (Km app and Vmax) had great variation. Our results using GTP showed that the great variation in kinetic parameters can be accounted for by post-translational modifications.

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“…The changes in ODC activity and polyamine synthesis have been followed in Manduca sexta (Birnbaum and Whelan, 1988), Tenebrio molitor (Besson et al, 1986), Hyalophora cecropia (Wyatt et al, 1973), Drosophila melanogaster (Birnbaum and Gilbert, 1990), and Aedes aegypti (Kogan and Hagerdorn, 2000). Moreover, some studies have associated normal vitellogenesis with ODC activity, which in Drosophila reaches a peak at 24 hours following adult eclosion and is reduced at 48 hours.…”

Section: Resultsmentioning

confidence: 99%

Effect of α-difluormethylornithine on Anastrepha fraterculus (Diptera, Tephritidae) ovary size

2009

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Ovarian sizes (length and width) were measured in young females of Anastrepha fraterculus (Wiedemann) (Diptera, Tephritidae) subjected or not to the inhibitor α-difluormethylornithine (α-DFMO). The most effective concentration of α-DMFO used was 50 mM and the ovarian measurements (length and width) of the treated females were smaller than those of females not treated with α-DMFO. These data may suggest some relationship between ornithine decarboxylase (ODC) and sexual maturation in A. fraterculus.Keywords: Anastrepha fraterculus, α-difluormethylornithine, ovarian size . Efeito da α-difluorometilornitina nas dimensões dos ovários emAnastrepha fraterculus (Diptera, Tephritidae) ResumoAs dimensões dos ovários (comprimento e largura) foram mensuradas em fêmeas jovens da Anastrepha fraterculus (Wiedemann) (Diptera, Tephritidae) submetidas ou não ao inibidor α-difluormetilornitina (α-DFMO). A concentração mais efetiva de α-DMFO utilizada foi 50 mM e as medidas (comprimento e largura) das fêmeas tratadas com o inibidor foram menores que as fêmeas não tratadas com inibidor α-DMFO. Estes dados podem sugerir uma relação entre ornitina descarboxilase (ODC) e maturação sexual em A. fraterculus.Palavras-chave: Anastrepha fraterulus, α-difluoro metil ornitina; tamanho do ovário.

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Ornithine decarboxylase and polyamines in silkmoth pupal tissues: Effects of ecdysone and injury

Cited by 53 publications

References 29 publications

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Ornithine decarboxylase activity during the development of Anastrepha fraterculus (Diptera, Tephritidae)

Effect of α-difluormethylornithine on Anastrepha fraterculus (Diptera, Tephritidae) ovary size

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