Ornamenting 3D printed scaffolds with cell-laid extracellular matrix for bone tissue regeneration

Pati, Falguni; Song, Tae-Ha; Rijal, Girdhari; Jang, Jinah; Kim, Sung Won; Cho, Dong‐Woo

doi:10.1016/j.biomaterials.2014.10.012

Cited by 308 publications

(217 citation statements)

References 55 publications

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“…ECM from various sources have been studied previously as biomaterials for tissue regeneration, as ECM has a number of obvious advantages for tissue engineering applications [17,35,43,44]. The data presented in this study add to the body of work demonstrating the utility of different cell and native tissue-derived ECM to be used as scaffolds in orthopaedic medicine [21,25,[45][46][47][48][49][50][51][52].…”

Section: Discussionmentioning

confidence: 75%

Porous decellularized tissue engineered hypertrophic cartilage as a scaffold for large bone defect healing

et al. 2015

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Clinical translation of tissue engineered therapeutics is hampered by the significant logistical and regulatory challenges associated with such products, prompting increased interest in the use of decellularized extracellular matrix (ECM) to enhance endogenous regeneration. Most bones develop and heal by endochondral ossification, the replacement of a hypertrophic cartilaginous intermediary with bone. The hypothesis of this study is that a porous scaffold derived from decellularized tissue engineered hypertrophic cartilage will retain the necessary signals to instruct host cells to accelerate endogenous bone regeneration. Cartilage tissue (CT) and hypertrophic cartilage tissue (HT) were engineered using human bone marrow derived mesenchymal stem cells, decellularized and the remaining ECM was freeze-dried to generate porous scaffolds. When implanted subcutaneously in nude mice, only the decellularized HT-derived scaffolds were found to induce vascularization and de novo mineral accumulation. Furthermore, when implanted into critically-sized femoral defects, full bridging was observed in half of the defects treated with HT scaffolds, while no evidence of such bridging was found in empty controls. Host cells which had migrated throughout the scaffold were capable of producing new bone tissue, in contrast to fibrous tissue formation within empty controls. These results demonstrate the capacity of decellularized engineered tissues as 'off-the-shelf' implants to promote tissue regeneration.

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Section: Discussionmentioning

confidence: 75%

Porous decellularized tissue engineered hypertrophic cartilage as a scaffold for large bone defect healing

et al. 2015

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“…More exciting than expected, a cohesive cell-formed sheet can be layered into 3D tissues or organs with physiological mechanical strength [715,716]; this concept has been demonstrated to be feasible in clinical sittings [717]. In addition, there is a wealth of evidence that cell-formed ECM products can be applied to enhance the large-scale expansion of highly functional adult human MSCs [718] or to decorate the surfaces of synthetic polymers and manufactured metals on which subsequent cell adhesion is regulated by adsorbed ECM proteins [96,184,719,720]. It is self-evident that the physical properties of synthetic biomaterials can be extensively tailored, but it is also clear that these materials often suffer from limited biological functionalities.…”

Section: Future Directions and Outlookmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

859

470

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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“…Many groups develop 3D synthetic polymeric biocompatible and biodegradable materials allowing osteoinduction. [42][43][44] This 3D environment is favorable to proliferation, cell differentiation, and mineralization, when equipped with a large amount of single cells. The classical tissue-engineering strategy using single dispersed cells on a biocompatible biomaterial has been predominant over the last 20 years.…”

Section: Discussionmentioning

confidence: 99%

Nanoengineered implant as a new platform for regenerative nanomedicine using 3D well-organized human cell spheroids

Keller¹,

Idoux-Gillet²,

Wagner³

et al. 2017

IJN

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Ornamenting 3D printed scaffolds with cell-laid extracellular matrix for bone tissue regeneration

Cited by 308 publications

References 55 publications

Porous decellularized tissue engineered hypertrophic cartilage as a scaffold for large bone defect healing

Porous decellularized tissue engineered hypertrophic cartilage as a scaffold for large bone defect healing

Advancing biomaterials of human origin for tissue engineering

Nanoengineered implant as a new platform for regenerative nanomedicine using 3D well-organized human cell spheroids

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