Ormona® SI and Ormona® RC—New Nutraceuticals with Geranylgeraniol, Tocotrienols, Anthocyanins, and Isoflavones—Decrease High-Fat Diet-Induced Dyslipidemia in Wistar Rats

Rodrigues, Ana Paula Santos; Barbosa, Raimundo da Silva; Pereira, Arlindo César Matias; Batista, Mateus Alves; Sales, Priscila Faimann; Ferreira, Adriana Maciel; Colares, Nayara Nilcia Dias; Silva, Heitor Ribeiro da; Soares, Marcelo Oliveira dos Santos; Hage‐Melim, Lorane Izabel da Silva; Carvalho, José Carlos Tavares

doi:10.3390/nutraceuticals2040024

Cited by 7 publications

(10 citation statements)

References 34 publications

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“…In addition, no deaths occurred in the group treated with Ormona ® at a 500 mg/kg dose. This study's histopathological analysis of the liver demonstrated that Ormona ® did not induce significant toxicity at 500 mg/kg [11].…”

Section: Discussionmentioning

confidence: 67%

“…Among the isoflavones found in the T. pratense species, four bind competitively to ERα and ERβ receptors (genistein > daidzein > biochanin A > formononetin) with affinities approximately one-thousandth of 17β-estradiol [41,42]. In the phytonutraceutical Ormona ® , 5.4% daidzein, 2.3% glycitein, and 3.6% genistein were identified [11], justifying the potent estrogenic activity performed by Ormona ® .…”

Section: Discussionmentioning

confidence: 97%

“…The chemical characterization of Ormona ® reveals a fraction rich in isoflavones, corresponding to 20.8% w/w (derived from Trifolium pratense extract). The HPLC analysis of the product identified 5.4% daidzein, 2.3% glycitein, and 3.6% genistein [11]. The chosen dose of Ormona ® was adapted from Tsuang et al [12].…”

Section: Experimental Design and Ovariectomymentioning

confidence: 99%

“…In addition, it contains 20.8% red clover extract (Trifolium pratense), standardized with isoflavones such as daidzein, genistein, Biochanin A, and formononetin. Another 10% of the formulation is composed of a standardized extract of açaí (Euterpe oleracea) standardized with anthocyanins (mainly cyanidin 3-Oglucoside) [11]. This study examined the impacts of Ormona ® on Wistar rats affected by ovariectomy-induced osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

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Osteoprotective Effect of the Phytonutraceutical Ormona® on Ovariectomy-Induced Osteoporosis in Wistar Rats

do Nascimento,

Furtado,

Vilhena

et al. 2024

Nutraceuticals

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The phytonutraceutical Ormona® is a product composed of purified oil of Bixa orellana Linné, dry extract of Myrciaria dubia McVaugh, dry extract of Trifolium pratense L., and dry extract of Euterpe oleracea Mart. obtained using Evolve® technology. This study evaluated the effects of Ormona® on Wistar rats affected by ovariectomy-induced osteoporosis. Pre-treatment was conducted for 15 days before surgery and continued for a further 45 days after the surgical procedure. The experimental design consisted of five groups (n = 5): OVW: treated with distilled water (1 mL/kg, p.o); ADS: alendronate sodium (4 mg/kg p.o); EST: conjugated estrogen (2 µg/kg, p.o); ORM: Ormona® (20 mg/kg, p.o); ORM + EST: Ormona® (20 mg/kg, p.o) + conjugated estrogen (2 µg/kg, p.o). Biochemical and hormonal parameters of bone histopathology and trabecular and femoral diaphysis size were evaluated through scanning electron microscopy (SEM) and bone calcium quantification by atomic absorption spectrophotometry. The results show that ovariectomy caused bone alterations such as loss of femoral microarchitecture, decreased bone homeostasis parameters, and changes in the lipid profile. Estrogen supplementation reduced parameters such as cholesterol, LDL, and Ca2+ concentration. However, Ormona® showed higher serum estradiol levels (p < 0.01), effects on the lipid profile, including parameters that estrogen replacement and alendronate sodium did not affect, with an increase in HDL, and positive modulation of bone metabolism, increasing osteocytes and the presence of osteoblasts. Ormona®, therefore, produced better results than the groups treated with estrogen and alendronate sodium.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 97%

Section: Experimental Design and Ovariectomymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Osteoprotective Effect of the Phytonutraceutical Ormona® on Ovariectomy-Induced Osteoporosis in Wistar Rats

do Nascimento,

Furtado,

Vilhena

et al. 2024

Nutraceuticals

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, it contains 20.8% red clover extract (Trifolium pratense), standardized with isoflavones such as daidzein, genistein, Biochanin A, and formononetin. 10% of the formulation is composed of a standardized extract of açaí (Euterpe oleracea) standardized with anthocyanins (mainly cyanidin 3-O-glucoside) (10).…”

Section: Discussionmentioning

confidence: 99%

Osteoprotective Effect of the Phytonutraceutical Ormona® on Post-Menopausal Osteoporosis in Wistar Rats

Do Nascimento,

Furtado,

Vilhena

et al. 2023

Preprint

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The phytonutraceutical Ormona® is a product composed of: purified oil of Bixa orellana Linné; dry extract of Myrciaria dubia McVaugh; dry extract of Trifolium pratense L. and dry extract of Euterpe oleracea Mart. obtained using Evolve® technology. This study evaluated the effects of Ormona® on Wistar rats affected by ovariectomy-induced osteoporosis. Pre-treatment was conducted for 15 days before surgery and continued for a further 45 days after the surgical procedure. The experimental design consisted of 5 groups (n=5): OVW: treated with distilled water (1 mL/ kg, p.o); ADS: alendronate sodium (4 mg/ kg p.o); EST: conjugated estrogen (2 ug/ kg, p.o); ORM: Ormona® (20 mg/kg, p.o); ORM + EST: Ormona® (20 mg/kg + conjugated estrogen 2 ug/ kg, p.o. Biochemical and hormonal parameters, along with bone histopathology and trabecular and femoral diaphysis size, were evaluated through Scanning Electron Microscopy (SEM) and bone calcium quantification by atomic absorption spectrophotometry. The results show that ovariectomy caused bone alterations such as loss of femoral microarchitecture, decreased bone homeostasis parameters, and changes in the lipid profile. Estrogen supplementation reduced parameters such as cholesterol, LDL, and Ca2+ concentration. However, Ormona® showed higher serum estradiol levels (p&lt; 0.01), effects on the lipid profile, including parameters that estrogen replacement and alendronate sodium did not affect, with an increase in HDL, and positive modulation of bone metabolism, increasing osteocytes and the presence of osteoblasts. Ormona®, therefore, produced better results than the groups treated with estrogen and alendronate sodium.

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Intramuscular compatibility of an injectable anti-inflammatory nanodispersion from a standardized Bixa orellana oil (Chronic®): a toxicological study in Wistar rats

et al. 2023

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Bixa orellana L. is a plant popularly known as "ucurum", "annatto", and "achiote". It is native to South America, and its seeds are an abundant source of geranylgeraniol and tocotrienols. Nanoencapsulation is a valuable technique that can decrease the drug needed to achieve an effect, decreasing potential toxicity and side effects. This study aimed to evaluate the acute toxicity of an intramuscular application of a nanodispersion containing a standardized extract (Chronic®) from the seeds of Bixa orellana (NBO) in Wistar rats. The chemical evaluation showed δ-tocotrienol at 0.725 ± 0.062 mg/ml (72.6 ± 0.9%). The stability study showed the nanoparticles had an average size from 53.15 ± 0.64 nm to 59.9 ± 3.63 nm, with a polydispersity index ranging from 0.574 ± 0.032 to 0.574 ± 0.32, Zeta potential from 18.26 ± 0.59 mV to 19.66 ± 1.45 mV. After testing the intramuscular application of NBO with doses from 1 to 5 mg/kg in animals, it was observed that the acute treatment did not elicit any toxic effects within this range. The dose of 10 mg/kg, although not affecting hematological and biochemical parameters (CPK, LDH, myoglobin, AST, ALT), could induce some muscle tissue changes, including leukocyte in ltration, morphological chances, and potentially necrosis. In conclusion, the results showed that the treatments devoided toxicity between 1 mg/kg and 5 mg/kg.

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Ormona® SI and Ormona® RC—New Nutraceuticals with Geranylgeraniol, Tocotrienols, Anthocyanins, and Isoflavones—Decrease High-Fat Diet-Induced Dyslipidemia in Wistar Rats

Cited by 7 publications

References 34 publications

Osteoprotective Effect of the Phytonutraceutical Ormona® on Ovariectomy-Induced Osteoporosis in Wistar Rats

Osteoprotective Effect of the Phytonutraceutical Ormona® on Ovariectomy-Induced Osteoporosis in Wistar Rats

Osteoprotective Effect of the Phytonutraceutical Ormona® on Post-Menopausal Osteoporosis in Wistar Rats

Intramuscular compatibility of an injectable anti-inflammatory nanodispersion from a standardized Bixa orellana oil (Chronic®): a toxicological study in Wistar rats

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