Origins, phylogenetic relationships and host-parasite interactions of Troglotrematoidea since the cretaceous

Vainutis, Konstantin S.; Voronova, Anastasia N.; Duscher, Georg Gerhard; Щелканов, Е. М.; Shchelkanov, M. Yu.

doi:10.1016/j.meegid.2022.105274

Cited by 2 publications

(1 citation statement)

References 42 publications

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“…Paragonimus is a single genus in the family Paragonimidae, which, together with the Nanophyetidae and Troglotrematidae, is part of the superfamily Troglotrematoidea (Blair et al 2016). There is debate not only about the Paragonimus genus but also about the ordal and superfamilial rank to which these species belong: whether the suborder Xiphidiata remains as originally classi ed or be reclassi ed as Troglotremata, all in the order Plagiorchiida (Blair 2008; 2019; Chai and Jung 2018; Vainutis et al 2022). In the taxonomic guide to infectious diseases, the suborder Troglotremata name is used where Paragonimus is a genus in the family Troglotrematidae, with the lineage being: Platyhelminthes: Trematoda ( ukes): Digenea: Plagiorchiida: Troglotremata (Berman, 2019).…”

Section: Introductionmentioning

confidence: 99%

Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): structure, polymorphism, and implications for intersubordal phylogeny

Nguyen,

Doan,

Pham

et al. 2024

Preprint

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The complete coding sequences (from the 5’ terminus of 18S to the 3’ terminus of 28S rRNA genes) of ribosomal transcription units (designated as rTU*) of seven strains of five Asian Paragonimus species, excluding the unsequenced flanking ETS and IGS regions, were genetically characterized. The rTU* length was 7,661 bp for P. heterotremus (LC strain, Vietnam), 7,543 bp for P. iloktsuenensis (Amami strain, Japan), 6,932 bp for P. skrjabini miyazakii (OkuST1 strain, Japan), 7,422 bp for P. ohirai (Kino strain, Japan), and 8,616 bp (Megha strain, India), 7,292 bp (Bogil strain, South Korea), and 7,052 bp (QT2 strain, Vietnam) for P. westermani geographical samples, respectively. All Paragonimus strains' rRNA genes were equal in length: 1,977 bp for 18S, 160 bp for 5.8S, and 3,881 bp for 28S, except for P. s. miyazakii’s 18S rRNA gene, which was 1,974 bp. The ITS2 region in all Paragonimus species was 283 − 285 bp. The ITS1 lengths varied from 634 bp (P. s. miyazakii) to 2,313 bp (Indian P. westermani, Megha strain) due to the presence of tandem repeats, except P. s. miyazakii. The structural polymorphism in ITS1 is defined by the existence of 120 bp- or 121 bp-repeat units. The ITS1 and ITS2 sequences were capable of forming de novo “hairpin” (or stem-loop) and “loop” secondary structures, and the ITS2 shared a characteristic “four fingers” motif, which is likely conserved in trematodes. The base composition of A, T, G, and C, as well as skewness values of AT and GC content for 18S, 28S, 18S + 28S, and rTU*, indicated that T, A, and C were used equally frequently, with G being more common than C. These usages resulted in low negative AT-skew and low positive GC-skew. The PhyML software package was used to construct a comprehensive maximum-likelihood phylogeny from the alignment of 161 sequences of 28S D1 − D3 rDNA (1.1–1.3 kb) from 101 species of 62 genera and 23 families. Of these, 75 sequences came from 18 species of 6 genera from the suborder Troglotremata (previously, Troglotrematoidea). The phylogenetic tree clearly showed a well-supported phylogeny and clarified relationships within Paragonimidae as well as between families within the Troglotremata and Xiphidiata, Echinostomata, and Haplosplanchnata suborders, where Paragonimidae is monophyletic. There were two significant clusters: one was mixed-Paragonimus with a very tight group of P. heterotremus strains, and the other was P. westermani/siamensis, which was seperated into eight geographical/country strain groupings. Xiphidiata appears to be polyphyletic. The ribosomal datasets presented here will be valuable for taxonomic reappraisal as well as evolutionary and population genetics studies in the superfamily Troglotrematoidea (or suborder Troglotremata).

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Section: Introductionmentioning

confidence: 99%

Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): structure, polymorphism, and implications for intersubordal phylogeny

Nguyen,

Doan,

Pham

et al. 2024

Preprint

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The Species Diversity Assessment of Azygia Looss, 1899 (Digenea: Azygiidae) from the Volga, Ob, and Artyomovka Rivers Basins (Russia), with Description of A. sibirica n. sp.

Vainutis

Voronova²,

Mironovsky

et al. 2023

Diversity

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This study is devoted to the investigation of Azygia (Digenea: Azygiidae) species diversity using classical morphological, recent molecular tools (28S rRNA and cox1 mtDNA for genetic-based inference) and robust statistical techniques (Principal component analysis, PCA). The analysis revealed that the genus Azygia included four valid species: A. lucii, A. longa, A. hwangtsiyui, and A. sibirica n. sp. The distribution of the type species A. lucii was confirmed in the largest Russian rivers: the Volga and the Ob. The worms isolated from Perccottus glenii were determined as the Chinese species A. hwangtsiyui, according to the genetic data for the cox1 mtDNA gene, at 1.32–1.56%. The new species, Azygia sibirica n. sp, was described from Esox lucius in the Ob River and differentiated from the type species A. lucii by the smaller ovary, testes and prostatic sac, wider body, very narrow pharyngeal lumen and form of anterior margin of ovary. In addition, multivariate analysis and three methods for species delimitation (ABGD, GMYC, bPTP) showed the subdivision of A. lucii and A. sibirica n. sp. into two separate groups, one from the Volga River and another from the Ob River, respectively. To conclude, A. lucii infects Esox lucius in the western (European part of Russia, the Volga River basin), and northern (Western Siberia, the Ob River basin) parts of Russia; A. sibirica n. sp. has also been found to infect Esox lucius in the Ob River, while A. hwangtsiyui infects Perccottus glenii in the South of the Russian Far East (the Artymovka River basin).

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Origins, phylogenetic relationships and host-parasite interactions of Troglotrematoidea since the cretaceous

Cited by 2 publications

References 42 publications

Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): structure, polymorphism, and implications for intersubordal phylogeny

Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): structure, polymorphism, and implications for intersubordal phylogeny

The Species Diversity Assessment of Azygia Looss, 1899 (Digenea: Azygiidae) from the Volga, Ob, and Artyomovka Rivers Basins (Russia), with Description of A. sibirica n. sp.

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