Original article Promising anticancer activity of lavender (Lavandulaangustifolia Mill.) essential oil through induction of both apoptosis and necrosis

Abstract

In the current study, potential anticancer and antiproliferative activity of EOs from lavender (Lavandula angustifolia Mill.) were determined against A549, H1299, C6 cancer cells and nontumorous HUVEC cells through induction of both apoptosis and necrosis. Furthermore, the differentiations of the cancer prevention activities of the EOs distilled from first and second year's harvest of the lavender growing under traditional system and plastic mulch were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue, neutral red uptake assays as well as lactate dehydrogenase (LDH) assay. The EOs exhibited remarkable anticancer and antiproliferative activity against all treated cancer cells, in a dose and time dependent manner. They exerted significantly decreases the cell growth in cancer cells, and also observed higher toxicity towards C6 glioma cells than the A549 and H1299 lung cells. In case of growing conditions of the EOs, higher anticancer and antiproliferative activity were observed under growing plastic mulch as compared to that of traditional system. Furthermore, first year's harvest of the lavender growing under plastic mulch seem to be possessed stronger cytotoxic effect than that of the second year's harvest. Besides, the lysosomal and LDH activity of the EOs were found to be highly in correlation with anticancer activity, and seem to be decreased obviously depending the year. It may be concluded that data obtained from the presented research demonstrates lavender EOs could have possible usage for cancer treatment as an alternative anticancer agent.

Apoptotic Effects on HL60 Human Leukaemia Cells Induced by Lavandin Essential Oil Treatment

“…Previous investigations on L. angustifolia EO have reported cytotoxic activity on different cancer cell lines, with EC 50 values of 80.62 ± 1.04 µg/mL on Hela cells and 88.90 ± 1.71 µg/mL on A549, confirming the high cytotoxic properties of the EOs from the Lamiaceae family on cancer cells, as observed in our results [9]. Gezici [27] determined that the cell growth and cell viability in three different cancer cell lines (A549, H1299, and C6) were affected by lavender (L. angustifolia Mill.) EOs at a low concentration and with minimum exposure time.…”

A study on Turmeric (Curcuma longa L.): Multifunctional agents for the management of oxidative damage, neurodegeneration and cancer

“…The A549 and HeLa cancer cells were cultured on Roswell Park Memorial Institute Medium (RPMI, ThermoFisher Scientific), and the other cells were grown in Dulbecco's modified Eagle medium (DMEM): Ham's F12 nutrient medium (1:1) (ThermoFisher Scientific) in the flasks at 37°C in a humidified CO 2 (5%) incubator. The cell growing conditions and supplements were used as same described in our previous publications Gezici, 2018;. In order to determine anticancer activities of the turmeric extracts, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) assay was performed as described by Mosmann (1983) 29 with minor modifications.…”

“…In order to determine anticancer activities of the turmeric extracts, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) assay was performed as described by Mosmann (1983) 29 with minor modifications. 12,16 The absorbance was measured at 570 nm with a Thermo Lab systems 408 Multiskan multiplate spectrophotometer, and the dose response curve was used to generate the IC 50 (μg mL −1 ) values for each cells.…”

“…capacities by our research group. [12][13][14][15][16][17][18][19][20][21][22][23][24][25] The main purpose of these researches are to contribute to discover new and effective natural products for prevention and treatment of oxidative stress-related diseases and disorders. Take into consideration our previous researches on MAPs and plant-derived natural products, total polyphenolic contents, in vitro antioxidant effects, neuroprotective and anticancer activities of the extracts obtained from the rhizomes of C. longa L. were aimed to evaluate in the presented research.…”

Anticancer, Antiproliferative and Lactate Dehydrogenase Enzyme Activities of Different Parts of Acorn (Quercus coccifera L.) against A549, MCF-7 and HeLa Human Cancer Cells

“…Meyveleri insan ve hayvan gıdası olarak kullanılan kermes meşesinin farklı bitki kısımları yüzyıllardır çeşitli hastalıkların tedavisinde halk ilacı olarak kullanılmakta, bitkinin meyvelerinin; sahip olduğu antimikrobiyal, antiülser, antihelmintik, antiinflamatuvar ve antioksidan aktivitelerinden dolayı, Anadolu Halk Hekimliğinde hemoroit, şeker hastalığı ve böbrek taşı rahatsızlıklarında kullanıldığı belirtilmektedir (Akcan ve ark., 2017; Sohretoglu ve ark., 2007). Çeşitli tıbbi ve etnobotanik kullanımlarının yanı sıra palamut adı verilen meşe meyveleri kavrulup öğütüldükten sonra farklı aroması ile kahve şeklinde de tüketilmektedir (Gezici ve Sekeroglu, 2019a Karik ve ark., 2018;Sekeroglu ve ark., 2018;Senol ve ark., 2018;Gezici, 2018Gezici, , 2019Gezici ve Sekeroglu, 2019a;2019b;Shida ve ark., 2019), meşe palamudunun (Q. coccifera L.) dış kabuk, külah ve iç kısımlarından elde edilen ekstraktların toplam polifenolik içerikleri, in vitro antioksidan kapasiteleri ve nöroprotektif aktiviteleri de önceki çalışmalarımızda tespit edilmiştir (Senol ve ark., 2018;Gezici ve Sekeroglu, 2019b Davis (1982) Türkiye Florası'na göre yapılmış olup; bitki materyali Biyoloji Bölümü Herbaryum'unda saklanmaktadır.…”