ORIGINAL ARTICLE: Pathomorphologic and Immunohistochemical Study on the Devastation of Rat Embryos by Antiphospholipid Antibody Positive Serum

Halperin, Reuvit; Elhayany, Asher; Ben-Hur, H; Gurevich, Pavel; Kaganovsky, Ella; Zusman, Igor; Shinnar, Nili; Hadas, Eran

doi:10.1111/j.1600-0897.2008.00650.x

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…36 Interestingly, a detrimental effect on rat embryo culture of locally injected under 30 kDa fraction from boiled aPL positive serum, that was abolished when purified IgG was injected, may indicate another as-yet unidentified factor leading to fetal loss. 37 Thus, the use of whole sera may suggest a more physiologic condition where multiple factors interplay together.…”

Section: Discussionmentioning

confidence: 99%

“…Halperin et al offered a novel factor in APS boiled and denaturated sera that is lower than 30 kDa and has a detrimental effect directed against embryonic cells without damage to the trophoblast cells. 37 In addition, during pregnancy of SLE/APS women the embryo develops from the beginning in a detrimental environment, here the embryos developed in a normal setting until 10.5 days, and were under stressful condition only for 28 h. A longer culture period may be needed in order to create a more stressful atmosphere as to get a more pronounced damaging effect.…”

Section: Discussionmentioning

confidence: 99%

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The embryotoxicity of sera from patients with autoimmune diseases on post-implantation rat embryos in culture persists during remission and is not related to oxidative stress

Ergaz¹,

Mevorach

Goldzweig

et al. 2010

Lupus

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We evaluated the embryolethality and embryotoxicity of sera from patients suffering from autoimmune diseases during remission on post-implantation rat embryos cultured on these sera and determined the association between the patients' clinical history, high blood levels of specific antibodies, medications, and oxidative stress parameters. One hundred and eighty, 10.5-day-old rat embryos were cultured in their yolk sacs in 33 sera of systemic lupus erythematosus (SLE)/antiphospholipid syndrome (APS) patients, and compared with 84 embryos cultured in rat sera and 88 embryos cultured in control human sera. The sera proved to be lethal and embryotoxic but not teratogenic resulting in smaller yolk sacs and embryos, lower protein level and lower developmental score. Significantly less embryos cultured in 'toxic' SLE/APS sera had peak 2 of low molecular weight antioxidants (LMWA) wave, implying a delayed maturation of the antioxidant defense. Lower peak 1 of LMWA correlated with a history of recurrent abortions. Embryonic levels of superoxide dismutase (SOD) and catalase (CAT) did not correlate with sera toxicity, patients' clinical history or specific antibodies. We conclude that SLE/APS patients' clinical remission did not prevent death or developmental delay accompanied by later appearance of peak 2 of LMWA in post-implantation rat embryo cultures. The normal levels of the antioxidant enzymes evaluated may indicate that sera toxicity is not related to oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The embryotoxicity of sera from patients with autoimmune diseases on post-implantation rat embryos in culture persists during remission and is not related to oxidative stress

Ergaz¹,

Mevorach

Goldzweig

et al. 2010

Lupus

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show abstract

“…They concluded that one possible mechanism of stillbirth as a result of anti-cardiolipin antibodies (aCL) was the induction of thrombotic events in the placenta. In addition to thrombosis, Halperin et al [30]. reported that the injection of human aPL positive sera into the uterus of pregnant rats rapidly and apoptotically destroyed intrauterine embryos, suggesting the existence of a novel factor in the aPL positive serum unrelated to aPL that exerts a detrimental effect to the conceptus without any relation to the aPL.…”

Section: Anti-phospholipid Antibody Syndrome and Rplmentioning

confidence: 99%

Effective Treatment for Recurrent Pregnancy Loss

Makino

2010

RIB

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Abstract:Understanding the etiology of recurrent pregnancy loss (RPL) has been increasing in importance in reproductive medicine, especially in societies with low birth rates. Since the cause of human miscarriage is often complicated and multifactorial, effective treatment for the successful maintenance of pregnancy requires the comprehensive clinical management of recurrent wastages. Immunology for the maintenance of pregnancy is a very attractive field; however, the role of immunology during human gestation cannot be confirmed in couples with a history of recurrent pregnancy loss unless other clinical causes of miscarriage are especially ruled out. Balanced chromosomal rearrangements have been found at an increased frequency in couples with recurrent pregnancy loss compared with the general population. The major anomalies are Robertosonian or reciprocal translocations, while most minor variants include pericentric inversion of chromosome 9. Both major and minor anomalies result in a spontaneous abortion rate of approximately 80-90%. Though the incidences of these chromosomal abnormalities vary in reported studies, a survey indicated an incidence of 9.8% (3.9% in male partners and 5.9% in female partners) among 1,639 couples with a history of recurrent pregnancy loss. Congenital uterine anomaly resulting in a suboptimal uterine environment may influence ovum implantation and early fetal development and may be a candidate in the etiology of reproductive wastage. A review on the relation between congenital uterine anomalies and the spontaneous abortion rate concluded that a precise diagnosis of uterine cavity deformity, especially arcuate uterus, is a key issue for determining the clinical prognosis during human gestation. After establishing diagnostic criteria for uterine cavity anomaly, 278 congenital uterine anomalies were detected in a series of 2,061 hysterosalpingographies (13.5%) performed in women with a history of recurrent pregnancy loss. Among these abnormalities, the incidences of spontaneous abortion were equally high among patients with arcuate uterus and those with partial septate uterus. A few studies on abortion mechanism in patients with congenital uterine anomalies have been reported. Histological findings using CD34-positive staining indicate the lack of a blood capillary system in both the uterine septum and the fundus muscle layer. The results of metroplastic surgery on congenital uterine anomaly are also controversial. Based on our fundamental findings of the maldistribution of uterine capillaries, we performed ideal metroplastic surgery in 173 women and significantly

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Lipid-binding antiphospholipid antibodies: significance for pathophysiology and diagnosis of the antiphospholipid syndrome

Müller-Calleja,

Ruf,

Lackner

2024

Critical Reviews in Clinical Laboratory Sciences

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ORIGINAL ARTICLE: Pathomorphologic and Immunohistochemical Study on the Devastation of Rat Embryos by Antiphospholipid Antibody Positive Serum

Abstract: These findings support the existence of a novel factor in the APLA-positive serum, causing a detrimental effect to the conceptus, without any relation to the antiphospholipid antibodies.

Cited by 5 publications

References 12 publications

The embryotoxicity of sera from patients with autoimmune diseases on post-implantation rat embryos in culture persists during remission and is not related to oxidative stress

The embryotoxicity of sera from patients with autoimmune diseases on post-implantation rat embryos in culture persists during remission and is not related to oxidative stress

Effective Treatment for Recurrent Pregnancy Loss

Lipid-binding antiphospholipid antibodies: significance for pathophysiology and diagnosis of the antiphospholipid syndrome

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