Original article High resolution proton nuclear magnetic resonance (1H NMR) spectroscopy of surviving C6 glioma cells after X-ray irradiation

Matulewicz, Ł.; Cichoń, Anna; Sokół, Maria; Przybyszewski, Waldemar M.; Głowala-Kosińska, Magdalena; Gibas, Mirosław

doi:10.5114/fn.2013.34194

Cited by 2 publications

(3 citation statements)

References 46 publications

(73 reference statements)

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“…Following treatment with antitumor glycosides, an increase in free choline and PC was reported in C6 glioma tumors in mice, suggesting that the mode of action of glycosides involves alterations in phospholipid metabolism resulting in cell death . X‐ray irradiation as well as treatments with the nitrones PBN and OKN007, both induced a significant decrease in the level of lipids related to the modulation of extent of necrosis .…”

Section: Preclinical Studiesmentioning

confidence: 99%

“…Response to multiple therapeutic approaches has been evaluated in preclinical in vivo models of GBM using 1 H MRS . Among these, treatment with the phosphatidylinositol‐3‐kinase (PI3K) irreversible inhibitor, PX‐886, resulted in a decrease in the MRS‐detectable Cho‐to‐NAA ratio, which was linked to increased autophagy post‐treatment .…”

Section: Preclinical Studiesmentioning

confidence: 99%

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Magnetic Resonance (MR) Metabolic Imaging in Glioma

Chaumeil¹,

Lupo²,

Ronen

2015

Brain Pathology

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This review is focused on describing the use of magnetic resonance (MR) spectroscopy for metabolic imaging of brain tumors. We will first review the MR metabolic imaging findings generated from preclinical models, focusing primarily on in vivo studies, and will then describe the use of metabolic imaging in the clinical setting. We will address relatively well-established (1) H MRS approaches, as well as (31) P MRS, (13) C MRS and emerging hyperpolarized (13) C MRS methodologies, and will describe the use of metabolic imaging for understanding the basic biology of glioma as well as for improving the characterization and monitoring of brain tumors in the clinic.

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Section: Preclinical Studiesmentioning

confidence: 99%

Section: Preclinical Studiesmentioning

confidence: 99%

Magnetic Resonance (MR) Metabolic Imaging in Glioma

Chaumeil¹,

Lupo²,

Ronen

2015

Brain Pathology

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“…The CH 2 /CH 3 ratio increased from 1.25 (non-irradiated cells) to 1.61 (irradiated cells). Increase in the lipid signals, especially increase in CH 2 / CH 3 ratio, is frequently claimed to be associated with progression of apoptotic death (Santini et al, 2006a;Santini et al, 2006b;Rainaldi et al, 2008;Matulewicz et al, 2013) and with disruption of fatty acid metabolism (Goudarzi et al, 2014). Blankenberg et al (Blankenberg et al, 1997) observed that in lymphoblasts treated with doxorubicin the onset of apoptosis was accompanied by over twofold increase in the CH 2 resonance.…”

Section: Discussionmentioning

confidence: 99%

Nuclear magnetic resonance spectroscopy reveals metabolic changes in living cardiomyocytes after low doses of ionizing radiation

Gramatyka¹,

Skorupa²,

Sokół³

2018

Acta Biochim Pol

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Several lines of evidence indicate that exposure of heart to ionizing radiation increases the risk of cardiotoxicity manifested by heart dysfunction and cardiovascular diseases. It was initially believed that the heart is an organ relatively resistant to radiation. Currently, however, it is suspected that even low doses of radiation (< 2 Gy) may have a negative impact on the cardiovascular system. Cardiotoxicity of ionizing radiation is associated with metabolic changes observed in cardiac cells injured by radiation. In this study, we used human cardiomyocytes as a model system, and studied their metabolic response to radiation using high-resolution magic angle spinning nuclear magnetic resonance techniques (HR-MAS NMR). Human cardiomyocytes cultured in vitro were exposed to ionizing radiation and their survival was assessed by clonogenic assay. Changes in apoptosis intensity and cell cycle distribution after the irradiation were measured as well. NMR spectra of cardiomyocytes were acquired using Bruker Avance 400 MHz spectrometer at a spinning rate of 3200 Hz. Survival of cardiomyocytes after NMR experiments was assessed by the Trypan blue exclusion assay. Exposure of cardiomyocytes to small doses of ionizing radiation had no effect on cell proliferation potential and intensity of cell death. However, analysis of metabolic profiles revealed changes in lipids, threonine, glycine, glycerophosphocholine, choline, valine, isoleucine, glutamate, reduced glutathione and taurine metabolism. The results of this study showed that ionizing radiation affects metabolic profiles of cardiomyocytes even at low doses, which potentially have no effect on cell viability.

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Original article High resolution proton nuclear magnetic resonance (1H NMR) spectroscopy of surviving C6 glioma cells after X-ray irradiation

Cited by 2 publications

References 46 publications

Magnetic Resonance (MR) Metabolic Imaging in Glioma

Magnetic Resonance (MR) Metabolic Imaging in Glioma

Nuclear magnetic resonance spectroscopy reveals metabolic changes in living cardiomyocytes after low doses of ionizing radiation

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