ORIGINAL ARTICLE: Endometrial Osteopontin mRNA Expression and Plasma Osteopontin Levels are Increased in Patients with Endometriosis

Cho, SiHyun; Ahn, Young Sun; Choi, Young Sik; Seo, Seok Kyo; Nam, Anna S.; Kim, Hye Yeon; Kim, Jae Hoon; Park, Ki Hyun; Cho, Dong Jae; Lee, Byung Seok

doi:10.1111/j.1600-0897.2009.00692.x

Cited by 46 publications

(38 citation statements)

References 29 publications

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“…Our findings on OPN gene expression are in agreement with other reports which have demonstrated an increase in OPN expression in a rat model of endometriosis in comparison to normal rats endometrial tissue and in patients with endometriosis in comparison with control subjects (28, 51-53). According to the functional role of OPN in cell adhesion, migration, differentiation and invasion of tumor cells, it could be assumed that OPN enhance endometrial invasiveness, proliferation and survival in ectopic lesions in animal models.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells

Jafarabadi

Salehnia

Sadafi

2017

IJRM

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Background:The animal models of endometriosis could be a valuable alternative tool for clarifying the etiology of endometriosis.Objective: In this study two endometriosis models at the morphological and molecular levels was evaluated and compared.Materials and Methods:The human endometrial tissues were cut into small fragments then they were randomly considered for transplantation into γ irradiated mice as model A; or they were isolated and cultured up to fourth passages. 2×106 cultured stromal cells were transplanted into γ irradiated mice subcutaneously as model B. twenty days later the ectopic tissues in both models were studied morphologically by Periodic acid-Schiff and hematoxylin and eosin staining. The expression of osteopontin (OPN) and matrix metalloproteinase 2 (MMP2) genes were also assessed using real time RT-PCR. 17-β estradiol levels of mice sera were compared before and after transplantation. Results:The endometrial like glands and stromal cells were formed in the implanted subcutaneous tissue of both endometriosis models. The gland sections per cubic millimeter, the expression of OPN and MMP2 genes and the level of 17-β estradiol were higher in model B than model A (p=0.03).Conclusion:Our observation demonstrated that endometrial mesenchymal stromal cells showed more efficiency to establish endometriosis model than human endometrial tissue fragments.

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Section: Discussionsupporting

confidence: 93%

Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells

Jafarabadi

Salehnia

Sadafi

2017

IJRM

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“…Another study by the same group showed lower levels of osteopontin RNA messenger (mRNA) in women with moderate to severe endometriosis during the early secretory phase (Burney et al, 2007). On the other hand, Cho et al (2009) showed that higher osteopontin mRNA was expressed in the eutopic endometrium and in the plasma of women with endometriosis when compared to controls. However, the authors of these studies included fertile and infertile patients with endometriosis and during different periods of the cycle, precluding the comparison of their results with those found by Casals et al (2012) and those presented here.…”

Section: Discussionmentioning

confidence: 95%

“…In this context, the literature presents conflicting studies evaluating progesterone receptors (Attia et al, 2000;Igarashi et al, 2005;Shen et al, 2015), αvβ3 integrin (Ordi et al, 2003;Wei et al, 2009;Casals et al, 2012) and osteopontin (Burney et al, 2007;Cho et al, 2009;Wei et al, 2009;Casals et al, 2012) in the eutopic endometrium of women with endometriosis, especially with respect to disease-associated infertility. Moreover, to date, there is no study evaluating the expression of HB-EGF in these patients and no previous study simultaneously evaluated the expression of these five genes in infertile patients with endometriosis.…”

Section: Introductionmentioning

confidence: 99%

Expression of PGR, HBEGF, ITGAV, ITGB3 and SPP1 genes in eutopic endometrium of infertile women with endometriosis during the implantation window: a pilot study

Broi¹,

Júnior²,

Meola³

et al. 2017

JBRA Assisted Reproduction

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Objective: Alterations in endometrial receptivity may be involved in the etiopathogenesis of endometriosis-related infertility. The literature has suggested that patients with endometriosis present progestin resistance, which could affect embryo implantation. We question the presence of alterations in the expression of the progesterone receptor gene (PGR) and the genes related to endometriumembryo interaction regulated by progesterone. This pilot study compared the expression of PGR, HBEGF, ITGAV, ITGB3, and SPP1 genes in eutopic endometrium during the implantation window (IW) in infertile women with endometriosis with that observed in the endometrium of fertile and infertile controls.Methods: In this prospective case-control study, endometrial biopsies were performed during the IW in patients aged between 18 and 45 years old, with regular cycles and without endocrine/systemic dysfunctions, divided into endometriosis (END), infertile control (IC) and fertile control (FC) groups. Total RNA extraction, cDNA synthesis, and gene expression analysis by Real-Time PCR were performed. We assessed the size of the difference that our series was powered to detect.Results: From the 687 patients who underwent diagnostic videolaparoscopy or tubal ligation at the University Hospital, 130 were eligible. Of these, 32 had endometrial samples collected, with 17 confirmed in the IW. Fifteen samples (5 END, 5 IC and 5 FC) were analyzed. There was no significant difference in the expression of any studied gene. Our sample size allowed us to identify or discard large differences (two standard deviations) among the groups.Conclusion: Endometriosis doesn't cause large changes in the endometrial expression of PGR, HBEGF, ITGAV, ITGB3 and SPP1 during the IW.

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“…Wei et al in 2009 found no difference OPN expression in the eutopic endometrium of women with endometriosis during the mid-secretory phase [22]. Interestingly, in the same year, Cho et al found significantly increased OPN mRNA levels in eutopic endometrium of women with endometriosis [27].…”

Section: Cytokines and Growth Factorsmentioning

confidence: 90%

Endometriosis and infertility: biomarkers affecting implantation rate

Carvalho

Hui

Agarwal

2013

Expert Review of Obstetrics & Gynecology

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ORIGINAL ARTICLE: Endometrial Osteopontin mRNA Expression and Plasma Osteopontin Levels are Increased in Patients with Endometriosis

Abstract: Osteopontin may be involved in the pathogenesis of endometriosis and plasma OPN may be a useful non-invasive marker for the diagnosis of endometriosis.

Cited by 46 publications

References 29 publications

Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells

Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells

Expression of PGR, HBEGF, ITGAV, ITGB3 and SPP1 genes in eutopic endometrium of infertile women with endometriosis during the implantation window: a pilot study

Endometriosis and infertility: biomarkers affecting implantation rate

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