Origin of Replication, oriC, of the Escherichia coli K12 Chromosome: Genetic Mapping and Minichromosome Replication

Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles

“…Initiation synchrony was found not to be affected by increasing the oriC concentration by introducing minichromosomes (unpublished results), despite their fairly high copy numbers (20,36). This result is complementary to the recent finding of precisely timed initiation of chromosome and minichromosome replication (17,19).…”

Replication of mini-P1 plasmid DNA in vitro requires two initiation proteins, encoded by the repA gene of phage P1 and the dnaA gene of Escherichia coli.

“…1); it was grown in E. coli strain N100 (13). The oriC plasmid was pCM960 grown in E. coli strain CM987 (14). The oriX plasmid DNA was RF I (replicative form I; i.e., superhelical circular doublestranded) DNA prepared from E. coli strain JM101 (15) infected with M13 oriX1 (16 (19) by the deletion of the X N gene, was the gift of R. Fuller (Stanford University).…”

A broadening view of recombinational DNA repair in bacteria

“…The initiation of replication at oriC is dependent on the DnaA protein and requires new RNA and protein synthesis (von Meyenburg et al 1979). DNA replication occurring in the absence of protein synthesis is called stable DNA replication (SDR) (Kogoma & Lark 1970, 1975.…”