Origin of regenerated epithelium in cryopreserved tracheal allotransplantation

Mukaida, Takahiro; Shimizu, Nobuyoshi; Aoe, Motoi; Andou, Akio; Date, Hiroshi; Moriyama, Shigeharu

doi:10.1016/s0003-4975(98)00154-4

Cited by 38 publications

(23 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…21 Up until now, we have not found any demerit about the reduction of epithelium. 4 Mukaida et al 22 confirmed that the recipient epithelia migrated gradually from the anastomotic site, and the regenerated epithelia that are of recipient origin covered the allograft within about 50 days after transplantation.…”

Section: Discussionmentioning

confidence: 96%

Ultrastructural Changes in Cryopreserved Tracheal Grafts of Sprague-Dawley Rats

Yu¹,

Yu²,

Ding³

et al. 2009

ASAIO Journal

View full text Add to dashboard Cite

It has been reported that immunosuppressant-free tracheal transplantation can be achieved by using cryopreserved grafts. However, the ultrastructural changes of cryopreserved tracheal tissue remain unclear. This study investigated this issue in cryopreserved tracheal grafts of 30 Sprague-Dawley (SD) rats, divided into five groups (n = 6 rats/group). Tracheal rings removed from them were stored in a deep freezer at -85 degrees C for 1 week, 1 month, 3 months, or 6 months. Never frozen tracheal tissues were obtained from the control group. Ultrastructural changes in the cryopreserved tracheas of each group were observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). As the duration of cryopreservation lengthened, tracheal grafts were observed by SEM to develop an obviously speckled depletion of epithelium, accompanied by a loss of cilia in the remaining epithelium. TEM examination of chondrocytes that had been cryopreserved for 3 months revealed obvious injury. Cell membranes and nuclei were frequently observed to be ruptured, and fibrils appeared disorganized and randomly oriented. As the time of cryopreservation increased, ultrastructural injury to tracheal chondrocytes became more severe. The data indicated that the optimal maximum time for cryopreservation of tracheal grafts of SD rats might be <3 months with our methods.

show abstract

Section: Discussionmentioning

confidence: 96%

Ultrastructural Changes in Cryopreserved Tracheal Grafts of Sprague-Dawley Rats

Yu¹,

Yu²,

Ding³

et al. 2009

ASAIO Journal

View full text Add to dashboard Cite

show abstract

“…If the graft did not develop severe stenosis, it was extracted at 2 months after transplantation when the epithelium of the donor had been replaced by that of the recipient. 17 These experiments were carried out in accordance with the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 80-23, revised in 1985) and the guideline for experiments on animals (Japanese Association for Laboratory Animal Science).…”

Section: Methodsmentioning

confidence: 99%

Allowable warm ischemic time to tracheal extraction for allotransplantation of cryopreserved trachea

Kitamura

Shimizu

Aoe

et al. 2004

Jpn J Thorac Cardiovasc Surg

Self Cite

View full text Add to dashboard Cite

show abstract

“…Mukaida observed gradual replacement of graft epithelium by recipient epithelial cells after sixty days in a cryopreserved tracheal allograft [130].…”

Section: Implant Modificationmentioning

confidence: 99%

Tracheal Tissue Engineering

Birla

2014

Introduction to Tissue Engineering

View full text Add to dashboard Cite

Origin of regenerated epithelium in cryopreserved tracheal allotransplantation

Cited by 38 publications

References 12 publications

Ultrastructural Changes in Cryopreserved Tracheal Grafts of Sprague-Dawley Rats

Ultrastructural Changes in Cryopreserved Tracheal Grafts of Sprague-Dawley Rats

Allowable warm ischemic time to tracheal extraction for allotransplantation of cryopreserved trachea

Tracheal Tissue Engineering

Contact Info

Product

Resources

About