1990
DOI: 10.1073/pnas.87.18.7260
|View full text |Cite
|
Sign up to set email alerts
|

Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells.

Abstract: We previously reported that osteoclast-like cells were formed in cocultures of a mouse marrow-derived stromal cell line (ST2) with mouse spleen cells in the presence of la,25-dihydroxyvitamin D3 and dexamethasone. In this study, we developed a new coculture system to determine the origin of osteoclasts. When relatively small numbers of mononuclear cells (103-105 cells per well) obtained from mouse bone marrow, spleen, thymus, or peripheral blood were cultured for 12 days on the ST2 cell layers, they formed col… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4

Citation Types

14
577
2
12

Year Published

1992
1992
2015
2015

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 933 publications
(622 citation statements)
references
References 29 publications
14
577
2
12
Order By: Relevance
“…Osteoclasts, which are terminally differentiated cells derived from hematopoietic progenitors in the monocyte/macrophage lineage, play an essential role in bone homeostasis by carrying out the controlled bone resorption essential for bone remodeling (Udagawa et al 1990). Osteoclastogenesis is a multistep process that involves progenitor survival, differentiation to mononuclear preosteoclasts, fusion to form multinucleate mature osteoclasts, and activation of mature osteoclasts to produce cells capable of bone resorption (Xing et al 2012).…”
Section: Introductionmentioning
confidence: 99%
“…Osteoclasts, which are terminally differentiated cells derived from hematopoietic progenitors in the monocyte/macrophage lineage, play an essential role in bone homeostasis by carrying out the controlled bone resorption essential for bone remodeling (Udagawa et al 1990). Osteoclastogenesis is a multistep process that involves progenitor survival, differentiation to mononuclear preosteoclasts, fusion to form multinucleate mature osteoclasts, and activation of mature osteoclasts to produce cells capable of bone resorption (Xing et al 2012).…”
Section: Introductionmentioning
confidence: 99%
“…Ocs, the only cells capable of carrying out bone resorption, are multinucleated cells that form by fusion of bone marrow-derived mononuclear precursors (Udagawa et al 1990). It has been well established that Oc formation from monocyte Oc precursors (pOcs) is regulated by two essential cytokines: receptor activator of NF-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF;Felix et al 1990;Lacey et al 1998).…”
Section: Introductionmentioning
confidence: 99%
“…3 In vitro studies have shown that osteoclasts in both mouse and humans may form directly from precursor cell populations of monocytes and macrophages. 4 To date, at least two key molecules that are essential and sufficient to the promotion of osteoclastogenesis have been identified, that is, macrophage colony-stimulating factor (M-CSF) and the receptor for activation of NF-kB ligand (RANKL). 5 M-CSF, which is imperative for macrophage maturation, binds to its receptor on early osteoclast precursors, thereby providing signals required for their survival, proliferation, and differentiation.…”
Section: Introductionmentioning
confidence: 99%
“…5 M-CSF, which is imperative for macrophage maturation, binds to its receptor on early osteoclast precursors, thereby providing signals required for their survival, proliferation, and differentiation. 4,6 RANKL, on the other hand, binds to receptors for activation of NF-kB (RANK) and induces signals necessary for both differentiation and activation of osteoclasts. 2,7,8 In addition to M-CSF and RANKL, various cytokines and hormones have been shown to play roles in the differentiation of pluripotent osteoclast progenitors into mature multinucleated osteoclasts, 5,9 such as interleukin-1b (IL-1b), 10 interleukin-6 (IL-6), 11 interleukin-11, 12 transforming growth factor-b (TGF-b), 13 1a, 25-dihydroxyvitamin D 3, 14 glucocorticoids, 5 and tumor necrosis factor-a (TNF-a).…”
Section: Introductionmentioning
confidence: 99%