Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein

Gray, John C.; Kung, Shain‐dow; Wildman, S. G.; Sheen, S. J.

doi:10.1038/252226a0

Cited by 171 publications

(62 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tobacco (Nicotiana tabacum) is an amphidiploid plant, probably derived from the two progenitor species N. tomentosiformis and N. sylvestris (Gerstel, 1966;Gray et al, 1974). To determine whether all of the RISP genes from both progenitors are transcribed in amphidiploid tobacco, genomic DNAs isolated from N. tabacum, N. tomentosiformis, and N. sylvestris were characterized by DNA gel blot analysis using cDNA probes.…”

Section: Mitochondria! Risp Genes From Both Progenitors Are Transcribmentioning

confidence: 99%

Flower-enhanced expression of a nuclear-encoded mitochondrial respiratory protein is associated with changes in mitochondrion number.

et al. 1994

View full text Add to dashboard Cite

The mitochondrial Rieske iron-sulfur protein is an obligatory component of the respiratory electron transport chain that is encoded by a single-copy gene in mammals and fungi. In contrast, this protein is encoded by a small gene family in dicotyledonous tobacco and monocotyledonous maize. We cloned four cDNAs from tobacco that encode the mitochondrial Rieske iron-sulfur protein. These clones, along with a previously isolated cDNA, represent five independent members of the gene family that can be divided into three subfamilies. All of these genes were derived from the two progenitor species and were expressed in amphidiploid tobacco. The proteins encoded by these five genes are probably functional because they all contain the universally conserved hexyl peptides necessary for the 2Fe-2s cluster formation. The expression of the Rieske protein gene family is differentially regulated; a 6-to 11-fold higher leve1 of steady state transcripts was found in flowers than in leaves, stems, and roots. Members of at least two subfamilies were preferentially expressed in flowers, indicating that they share a common cis-regulatory element(s), which can respond to a flowerspecific signal(s). Although -10 times more transcripts occurred in flowers than in leaves, flower and leaf mitochondria contained a similar amount of the Rieske protein. Flowers, however, contained seven times more Rieske proteins than leaves. These results indicated an increase in mitochondrion number in flowers. High-energy demands during anther development might bring about an increase in mitochondrion numbers in flowers and the flower-enhanced expression of the Rieske protein gene family. Our results suggested that nuclear genes encoding mitochondrial respiratory proteins could sense and respond to changes in energy metabolism andlor changes in mitochondrion numbers. INTRODUCTIONThe mitochondrial electron transport chain of eukaryotes consists of four major multimeric enzyme complexes, one of which is the ubiquino1:cytochrome c oxidoreductase, commonly referred to as the cytochrome bcl complex or complex III. Electron flow through the cytochrome bcl complex is coupled with a vectorial transmembrane proton translocation, which generates an electrochemical gradient that is subsequently used for the synthesis of ATP by the Fo/Fl ATPase located in the mitochondrial inner membrane (for review, see Trumpower, 1981). The enzyme complex has been purified from bacteria (Yang and Trumpower, 1986), fungi (Siedow et al., 1978;Weiss and Kolb, 1979), plants (Nakajimaet al., 1984;Berry et al., 1991;Braun and Schmitz, 1992), and mammals (Rieske et al., 1964). Although the number of subunits in the complex varies from as few as three in some bacteria to as many as 11 in some higher eukaryotes, all bcl complexes contain a core of three catalytic subunits, including cytochrome b, which has two heme groups, cytochrome cl, and the Rieske iron-sulfur protein (RISP) that contains a nonheme 2Fe-2s cluster.To whom correspondence should be addressed.The RlSP was first observed in ...

show abstract

Section: Mitochondria! Risp Genes From Both Progenitors Are Transcribmentioning

confidence: 99%

Flower-enhanced expression of a nuclear-encoded mitochondrial respiratory protein is associated with changes in mitochondrion number.

et al. 1994

View full text Add to dashboard Cite

show abstract

“…Since the genetic code for the large subunit is maternally inherited (5) it was concluded that Nicotiana sylvestris is the female parent ofNicotiana tabacum. The small subunit of Nicotiana sylvestris, tomentosiformis and otophora give single bands with the isoelectric focusing procedure, whereas Nicotiana tabacum produces two bands of which one corresponds in its isoelectric point to that of Nicotiana sylvestris or otophora and the other to that of Nicotania tomentosiformis (12). Since the genetic code for the small subunit shows biparental inheritance in a Mendelian manner (17) the results of GRAY et al indicate that Nicotiana tabacum has retained the allelle of Nicotiana sylvestris as well as the allete of Nicotiana tomentosiformis at the gene locus coding for the small subunit of ribulose diphosphate carboxylase.…”

Section: S Strobaek El Al: Polymorphism Of Ribulose Diphosphate Carmentioning

confidence: 99%

“…4.1.1.39) by isoelectric focusing has been carried out by GRAY. KUNG, WILDMAN and SHEEN (12) for the large and small subunit of Nicotiana tabacum, sylvestris, tomentosiformis and otophora as well as newly produced amphidiploids of Nicotiana sylvestris x tomentosiformis and Nicotiana sylvestris x otophora. The large subunit of Nicotiana tabacum revealed three polypeptide bands with the same isoelectric points as the three bands from Nicotiana sylvestris, whereas the polypeptide pattern obtained from the large subunits of Nicotiana tomentosiformis or otophora were different.…”

Section: S Strobaek El Al: Polymorphism Of Ribulose Diphosphate Carmentioning

confidence: 99%

“…Turkish Samsun was described earlier (9). About 100 mg three times crystallized native protein from Nicotiana sylvestris or tomentosiformis obtained by the direct crystallization method of CHAN et al (4), with modification in the case of tomentosiformis (12), were dissolved in 25 ml 0.05 M Tris-HCl pH 8.5 buffer containing 0.5% sodium dodecyl sulphate and 0.14 M 13-mercaptoethanol, flushed with nitrogen and incubated at room temperature for 30 minutes. The subunits were then separated by sodium dodecyl sulphate column chromatography under conditions described earlier (22).…”

Section: Purification Of Ribulose-l5-diphosphate Carboxylase and Sepmentioning

confidence: 99%

“…small subunits configuration. The separations achieved by isoelectric focusing (12) are schematically shown in the columns beside the molecular models. The upper three bands originate from the large subunit, the lower from the small subunit.…”

Section: Amino Acid Analysismentioning

confidence: 99%

See 2 more Smart Citations

On the nature of the polymorphism of the small subunit of ribulose-1,5-diphosphate carboxylase in the amphidiploid Nicotiana tabacum

Strøbæk

Gibbons

Haslett

et al. 1976

Carlsberg Res. Commun.

Self Cite

View full text Add to dashboard Cite

The N-terminal amino acid sequence of the small subunit of ribulose-l,5-disphosphate carboxylase from the amphidiploid Nicotiana tabacum contains two polymorphisms. From examination of the equivalent sequences in the putative parent species Nicotiana sylvestris and tomentosiformis it is concluded that the amphidiploid Nicotiana tabacum has inherited two alleles for the small subunit of ribulose diphosphate carboxylase, one from each parent species. The alleles continue to be retained and expressed. The relevance of these findings is discussed in relation to the successful adaption of the amphidiploid Nicotiana tabacum to a wide range of environments.

show abstract

Analysis of the N Gene of Nicotiana

Dunigan

Golemboski

Zaitlin

2007

Ciba Foundation Symposium 133 ‐ Plant Resistance to Virus

View full text Add to dashboard Cite

Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein

Cited by 171 publications

References 10 publications

Flower-enhanced expression of a nuclear-encoded mitochondrial respiratory protein is associated with changes in mitochondrion number.

Flower-enhanced expression of a nuclear-encoded mitochondrial respiratory protein is associated with changes in mitochondrion number.

On the nature of the polymorphism of the small subunit of ribulose-1,5-diphosphate carboxylase in the amphidiploid Nicotiana tabacum

Analysis of the N Gene of Nicotiana

Contact Info

Product

Resources

About