Origin of metazoan adhesion molecules and adhesion receptors as deduced from cDNA analyses in the marine sponge Geodia cydonium : a review

Müller, Wernér E.G.

doi:10.1007/s004410050885

Cited by 102 publications

(53 citation statements)

References 72 publications

(67 reference statements)

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“…The transition from Protozoa to Metazoa dates back 600-1000 million years ago (reviewed in : Müller 1997;Nikoh et al 1997). It has been proposed that oxygen level, temperature, and seawater chemistry played major roles in the evolution from Protozoa to Metazoa (Seilacher 1994).…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of Spicule Formation in the Demosponge Suberites domuncula: Silicatein-Collagen-Myotrophin

Müller¹,

Krasko²,

Pennec³

et al. 2003

Silicon Biomineralization

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Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of Spicule Formation in the Demosponge Suberites domuncula: Silicatein-Collagen-Myotrophin

Müller¹,

Krasko²,

Pennec³

et al. 2003

Silicon Biomineralization

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“…The sponge MTL represents a tandem duplicated polypeptide composed of 2 parts which are highly related to metazoan MTs. This finding is not too surprising in view of our earlier studies, which revealed that the domain composition and the degree of domain duplication in the sponge genome is unusually complex in comparison to other metazoan phyla (reviewed in Miiller 1997Miiller , 1998. The second distinction between the sponge MTL and the metazoan MTs, which are usually constitutively expressed, especially in tissue undergoing growth and differentiation (Brady et al 19821, is the fact that the sponge MTL is an inducible protein.…”

Section: Discussionmentioning

confidence: 78%

Purification, cDNA cloning and expression of a cadmium-inducible cysteine-rich metallothionein-like protein from the marine sponge Suberites domuncula

Schröder¹,

Shostak²,

Gamulin³

et al. 2000

Mar. Ecol. Prog. Ser.

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During the last few years sponges have been proven to be suitable model organisms for biomonitoring programmes. Previously it was reported that the marine sponge Suberites domuncula reacts to cadmium, both under controlled laboratory conditions and in the field with an increased expression of the heat-shock protein 70 and with DNA damage. In this study it is shown that this sponge contains a cadmium-binding protein with a M, of 24 000. Cloning studies detected a gene in S. domuncula, termed SDMTL, which encodes a putative polypeptide, MTL-SUBDO, that shares high sequence similarity with metallothioneins (MTs) from Metazoa. The open reading frame displays a polypeptide with a calculated size of M, of 17 103. Phylogenetic analysis revealed that the sponge molecule, termed MT-like (MTL) protein, is related to the metazoan MTs. Under laboratory conditions, no expression of the SDMTL can be detected. After 1 d, incubation of the sponges with cadmium (200 pg 1-l) already revealed a dramatic upregulation of the MTL gene in S. domuncula. Subsequent studies in the field, the area around Rovinj (Croatia), demonstrated a correlation between the environmental load with cadmium (accumulation of this metal in the animals) and the expression of the gene encoding the 'detoxifying' cadmium-binding protein, MTL, in the test animals. These data suggest that the discovered MTL gene from S. domuncula is a sensitive indicator for environmental, bioavailable cadmium.

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“…There is only one report in the literature that assigns a definitive role for a sponge lectin, the Geodia cydonium lectin, which may act as a bridge linking the aggregation factor to cells (39). G. cydonium is the most characterized sponge lectin to date (6, 33, 40 -42), and it has been included recently (3,(43)(44)(45)(46) in the Galectins family (47)(48)(49)(50)(51)(52), based on DNA sequence homology. The Galectins are soluble proteins with a conserved S-type domain, which share the ability to bind ␤-galactosides in polylactosamine units.…”

Section: Discussionmentioning

confidence: 99%

Lectins from Tropical Sponges

Miarons

Fresno

2000

Journal of Biological Chemistry

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Only a few animal phyla have been screened for the presence and distribution of lectins. Probably the most intensively studied group is the mollusk. In this investigation, 22 species from 12 families of tropical sponges collected in Los Roques National Park (Venezuela) were screened for the presence of lectins. Nine saline extracts exhibited strong hemagglutinating activity against pronase-treated hamster red blood cells; five of these reacted against rabbit red blood cells, four with trypsintreated bovine red blood cells, and five with human red blood cells regardless of the blood group type. Extracts from the three species studied from genus Aplysina (archeri, lawnosa, and cauliformis) were highly reactive and panagglutinating against the panel of red blood cells tested. The lectins from A. archeri and A. lawnosa were purified to homogeneity by ammonium sulfate fractionation, affinity chromatography on p-aminobenzyl-␤-1-thiogalactopyranoside-agarose, and gel filtration chromatography. Both lectins exhibited a native molecular mass of 63 kDa and by SDS-polyacrylamide gel electrophoresis under reducing conditions have an apparent molecular mass of 16 kDa, thus suggesting they occur as homotetramers. The purified lectins contain 3-4 mol of divalent cation per molecule, which are essential for their biological activity. Hapten inhibition of hemagglutination was carried out to define the sugar binding specificity of the purified A. archeri lectin. The results indicate a preference of the lectin for nonreducing ␤-linked D-Gal residues being the best inhibitors of red blood cells binding methyl-␤-D-Gal and thiodigalactoside (Gal␤1-4-thiogalactopyranoside). The behavior of several glycans on immobilized lectin affinity chromatography confirmed and extended the specificity data obtained by hapten inhibition.In animals, only a few phyla have been screened for the presence and distribution of lectins. In particular, the number of lectins that have isolated from invertebrate organisms is quite small as compared with the great variety of lectins isolated from plant origin and have been limited to those partially characterized from mollusks and crustaceans. Since the discovery of hemagglutinins in sponges by Dodd et al. (1), there have been some reports on the partial characterization (serological and immunoelectrophoretic properties) of lectins from the oldest multicellular animals (2, 3) from the Mediterranean Sea or Japan (4 -8). However, their properties and specificities have not been clearly defined. In this report, we present data on an investigation undertaken to search for novel lectins in marine organisms, which could show unique properties. In addition, we describe the purification and characterization of the lectins present in two species of tropical sponges, Aplysina lawnosa and Aplysina archeri. We present information on the nature and specificity of their combining site as examined by hapten inhibition experiments and affinity chromatography.

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Origin of metazoan adhesion molecules and adhesion receptors as deduced from cDNA analyses in the marine sponge Geodia cydonium : a review

Cited by 102 publications

References 72 publications

Molecular Mechanism of Spicule Formation in the Demosponge Suberites domuncula: Silicatein-Collagen-Myotrophin

Molecular Mechanism of Spicule Formation in the Demosponge Suberites domuncula: Silicatein-Collagen-Myotrophin

Purification, cDNA cloning and expression of a cadmium-inducible cysteine-rich metallothionein-like protein from the marine sponge Suberites domuncula

Lectins from Tropical Sponges

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