Origin of endothelial progenitors in human postnatal bone marrow

Reyes, Morayma; Dudek, Arkadiusz Z.; Jahagirdar, Balkrishna; Koodie, Lisa; Marker, Paul H.; Verfaillie, Cathérine

doi:10.1172/jci0214327

Cited by 934 publications

(457 citation statements)

References 42 publications

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“…Another important factor regulating the fate of the plated cells is the cell culture media ranging from EBM-2 (23-25), medium-199 (22,26,27), to x-vivo-20 (28) as basal media, and 20% fetal calf serum (1), brain extract (1,26), to commercial available single quots of VEGF, insulin-like growth factor (IGF), basic fibroblast growth factor (23,25) as supplements. An identification criteria often used for EPCs in the culture of MNCs for 4 or 7 days is the double-positive staining of EPCs for endothelial-specific lectin (e.g., Ulex europaeus agglutini-1) and Dil-labeled acetylated low-density lipoproteins (Ac-LDL) (26,29,30). An example of such a staining is shown in Figure 3.…”

Section: Cell Culturementioning

confidence: 99%

Endothelial progenitor cells: Implications for cardiovascular disease

et al. 2008

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Section: Cell Culturementioning

confidence: 99%

Endothelial progenitor cells: Implications for cardiovascular disease

et al. 2008

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“…Early EPCs secrete large amounts of angiogenic growth factors (Rehman et al 2003;Hur et al 2004). Alternatively, late EPCs possess high proliferation potency and express CD34 (Hur et al 2004;Lin et al 2000;Reyes et al 2002). In addition, ex vivo cultivation procedures may confound the influence of cardiac risk factors on the number of EPCs, whereas the direct measurement of EPCs might more closely resemble in vivo conditions (George et al 2006).…”

Section: Determinants Of Circulating Cd14 + -Epcs Levelsmentioning

confidence: 99%

Level of CD14+-endothelial progenitor cells is not associated with coronary artery disease or cardiovascular risk factors

Qiang

Jiang

et al. 2008

AGE

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There is evidence for two subpopulations among circulating endothelial progenitor cells (EPCs), i.e., CD34 + -EPCs and CD14 + -EPCs. Prior studies on the relationship between the level of EPCs and coronary artery disease (CAD), either did not distinguish between the two types of EPCs or studied only CD34 + -EPCs. We therefore investigated whether the number of circulating CD14 + -EPCs correlates with either CAD and/or cardiovascular risk factors. Circulating CD14 + -EPCs-as defined by the surface markers CD14 + KDR + -were analyzed by flow cytometry in 100 individuals [34 control subjects, 41 patients with stable CAD and 25 patients with acute coronary syndromes (ACS)]. The level of circulating CD14 + -EPCs was not significantly different in patients with normal coronary arteries compared to those with stable CAD or ACS. Neither was there any association between the severity of CAD or risk factors and the number of circulating CD14 + -EPCs. Thus, the number of circulating CD14 + -EPCs was not significantly correlated either with the severity of coronary disease or with cardiovascular risk factors.

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“…Jiang and colleagues have isolated multipotent mesenchymal stem cells from adult mouse and human bone marrow and found these stem cells could produce mature endothelial cells in vitro and in vivo (Jiang et al, 2002;Reyes et al, 2002). The human stem cell antigen CD133 is expressed in the CD34 ϩ hematopoietic cells but not in mature endothelial cells.…”

Section: Adult Hemangioblasts In Mice and Humansmentioning

confidence: 99%

Molecular and developmental biology of the hemangioblast

Xiong

2008

Developmental Dynamics

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The hemangioblast hypothesis was proposed a century ago. The existence of hemangioblasts is now demonstrated in mouse and human embryonic stem cell (ESC) -derived embryoid bodies (EBs), in the mouse and zebrafish gastrula, and in adults. The hemangioblast is believed to derive from mesodermal cells, and is enriched in the Bry ؉ Flk1 ؉ and Flk1 ؉ Scl ؉ cell populations in EBs and in the posterior primitive streak of the mouse gastrula and in the ventral mesoderm of the zebrafish gastrula. However, recent studies suggest that the hemangioblast does not give rise to all endothelial and hematopoietic lineages in mouse and zebrafish embryos. Although several signaling pathways are known to involve the generation of hemangioblasts, it remains largely unknown how the hemangioblast is formed and what are the master genes controlling hemangioblast development. This review will summarize our current knowledge, challenges, and future directions on molecular and developmental aspects of the hemangioblast.

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Origin of endothelial progenitors in human postnatal bone marrow

Cited by 934 publications

References 42 publications

Endothelial progenitor cells: Implications for cardiovascular disease

Endothelial progenitor cells: Implications for cardiovascular disease

Level of CD14+-endothelial progenitor cells is not associated with coronary artery disease or cardiovascular risk factors

Molecular and developmental biology of the hemangioblast

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