Origin of C. latifolia and C. aurantiifolia triploid limes: the preferential disomic inheritance of doubled-diploid ‘Mexican’ lime is consistent with an interploid hybridization hypothesis

Rouiss, Houssem; Bakry, Frédéric; Froelicher, Yann; Navarro, Luís; Aleza, Pablo; Ollitrault, Patrick

doi:10.1093/aob/mcx179

Cited by 25 publications

(46 citation statements)

References 89 publications

(128 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The gamete proportions explained by random meiotic chromosome association (τ) ranged from 7 to 58%, and for all markers preferential pairing occurred between chromosomes derived from the same parent (i.e., WLM or PON). Such intermediate inheritance with a disomic tendency was also described for a doubled-diploid “Mexican lime” ( Rouiss et al, 2018 ) belonging to C. x aurantiifolia (Christm.) Swingle, a species of interspecific origin ( C. micrantha Wester x C. medica L.; Nicolosi et al, 2000 ; Curk et al, 2016 ; Wu et al, 2018 ).…”

Section: Discussionsupporting

confidence: 52%

“…For the last somatic hybrid, this cytogenetic observation was associated with preferential tetrasomic inheritance of molecular markers. However, as previously mentioned, reciprocal or inverted translocations can result in tetravalent formation even in diploid parents as observed in “Valencia” sweet orange ( Del Bosco et al, 1999 ) or Mexican lime ( Rouiss et al, 2018 ). Therefore, cytogenetic observations should be associated with marker segregation analysis for a full understanding of tetraploid meiosis behavior.…”

Section: Discussionmentioning

confidence: 96%

“…Weak or no preferential pairing of homologous chromosomes (i.e., mostly tetrasomic inheritance) with bivalents and quadrivalent meiotic configurations was observed for a mandarin + lemon somatic hybrid ( Kamiri et al, 2011 ), for a somatic hybrid between tangelo and pummelo ( Xie et al, 2015 ), and doubled-diploid clementine ( Aleza et al, 2016a ). By contrast, the doubled-diploid “Mexican” lime had predominantly disomic segregation ( Rouiss et al, 2018 ). However, no data were available on the inheritance mode of tetraploid Citrus-Poncirus intergeneric hybrids.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preferential Homologous Chromosome Pairing in a Tetraploid Intergeneric Somatic Hybrid (Citrus reticulata + Poncirus trifoliata) Revealed by Molecular Marker Inheritance

et al. 2018

View full text Add to dashboard Cite

The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop knowledge on the mode of inheritance in the intergeneric tetraploid hybrids. We assessed the meiotic behavior of an intergeneric tetraploid somatic hybrid resulting from symmetric protoplast fusion of diploid Citrus reticulata and diploid Poncirus trifoliata. The analysis was based on the segregation patterns of 16 SSR markers and 9 newly developed centromeric/pericentromeric SNP markers, representing all nine linkage groups of the Citrus genetic map. We found strong but incomplete preferential pairing between homologues of the same ancestral genome. The proportion of gametes that can be explained by random meiotic chromosome associations (τ) varied significantly between chromosomes, from 0.09 ± 0.02 to 0.47 ± 0.09, respectively, in chromosome 2 and 1. This intermediate inheritance between strict disomy and tetrasomy, with global preferential disomic tendency, resulted in a high level of intergeneric heterozygosity of the diploid gametes. Although limited, intergeneric recombinations occurred, whose observed rates, ranging from 0.09 to 0.29, respectively, in chromosome 2 and 1, were significantly correlated with τ. Such inheritance is of particular interest for rootstock breeding because a large part of the multi-trait value selected at the teraploid parent level is transmitted to the progeny, while the potential for some intergeneric recombination offers opportunities for generating plants with novel allelic combinations that can be targeted by selection.

show abstract

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preferential Homologous Chromosome Pairing in a Tetraploid Intergeneric Somatic Hybrid (Citrus reticulata + Poncirus trifoliata) Revealed by Molecular Marker Inheritance

et al. 2018

View full text Add to dashboard Cite

show abstract

“…For example, Navarro et al (2015) showed that the 'Fortune' mandarinwhen used as a female parent -produced a greater frequency of triploid hybrids per fruit than clementines. In citrus cultivars, triploid hybrids have been successfully used to produce seedless fruits (Aleza et al, 2012;Rouiss et al, 2018). Despite their desirable characteristics, such as larger fruit and higher yield (Hoshino et al, 2011), triploid plants are unusual because of their inviable seeds.…”

Section: Introductionmentioning

confidence: 99%

Triploid Citrus Genotypes Have a Better Tolerance to Natural Chilling Conditions of Photosynthetic Capacities and Specific Leaf Volatile Organic Compounds

Lourkisti

Froelicher

Herbette³

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Low temperatures during winter are one of the main constraints for citrus crop. Polyploid rootstocks can be used for improving tolerance to abiotic stresses, such as cold stress. Because the produced fruit are seedless, using triploid scions is one of the most promising approaches to satisfy consumer expectations. In this study, we evaluated how the triploidy of new citrus varieties influences their sensitivity to natural chilling temperatures. We compared their behavior to that of diploid citrus, their parents (Fortune mandarin and Ellendale tangor), and one diploid clementine tree, as reference, focusing on photosynthesis parameters, oxidative metabolism, and volatile organic compounds (VOC) in leaves. Triploid varieties appeared to be more tolerant than diploid ones to natural low temperatures, as evidenced by better photosynthetic properties (P net , g s , F v /F m , ETR/P net ratio), without relying on a better antioxidant system. The VOC levels were not influenced by chilling temperatures; however, they were affected by the ploidy level and atypical chemotypes were found in triploid varieties, with the highest proportions of E-β-ocimene and linalool. Such compounds may contribute to better stress adaptation.

show abstract

“…Comparatively, we found that for 'Moncada' mandarin as female parent, the LG6 shows tetrasomic segregation, while results for the LG8 agree with the after as was reported for the tetraploid 'Clemenules' clementine, but with higher PP value. Subsequently, Rouiss et al (2018) analyzed the segregation model of the tetraploid 'Mexican' lime (C. aurantiifolia), which originated from an interspecific hybridization between C. micrantha (papeda) and C. medica (Citron) (Curk et al 2016;Nicolosi et al 2000;Wu et al 2018). The results showed that tetraploid 'Mexican' lime has intermediary inheritance with a preferential disomic trend.…”

Section: Preferential Pairing (Pp) and Maximum Double Reduction (Dr)mentioning

confidence: 99%

Male and female inheritance patterns in tetraploid ‘Moncada’ mandarin

et al. 2019

View full text Add to dashboard Cite

Key message Tetraploid `Moncada´ mandarin, used as male and female in interploidy hybridizations, displays mainly tetrasomic inheritance for most LGs, with slight variations according to the direction of the crossing. Abstract Triploid-breeding programs in citrus are key tool to develop seedless cultivars. Obtaining triploid citrus hybrids may be achieved through different strategies, such as the exploitation of female unreduced gamete in crosses between diploid parents and diploid by tetraploid sexual hybridizations, in which tetraploid genotypes can be used as male or female parents. Genetic configuration of triploid populations from interploid crosses greatly depends on the chromosomic segregation mode of the tetraploid parent used. Here, we have analyzed the inheritance of the tetraploid 'Moncada' mandarin and compared the genetic structures of the resulting gametes when used as male and as female parent. The preferential chromosome pairing rate is calculated from the parental heterozygosity restitution (PHR) of codominant molecular markers, indicating the proportion between disomic and tetrasomic segregation. Tetraploid 'Moncada' both as female and male parent largely exhibited tetrasomic segregation. However, as female parent, one linkage group (LG8) showed intermediate segregation with tendency towards tetrasomic inheritance, while another linkage group (LG4) evidenced a clear intermediate segregation. On the other hand, when used as male parent two linkage groups (LG5 and LG6) showed values that fit an intermediate inheritance model with tetrasomic tendency. Significant doubled reduction (DR) rates were observed in five linkage groups as female parent, and in six linkage groups as male parent. The new knowledge generated here will serve to define crossing strategies in citrus improvement programs to efficiently obtain new varieties of interest in the global fresh consumption market.

show abstract

Origin of C. latifolia and C. aurantiifolia triploid limes: the preferential disomic inheritance of doubled-diploid ‘Mexican’ lime is consistent with an interploid hybridization hypothesis

Cited by 25 publications

References 89 publications

Preferential Homologous Chromosome Pairing in a Tetraploid Intergeneric Somatic Hybrid (Citrus reticulata + Poncirus trifoliata) Revealed by Molecular Marker Inheritance

Preferential Homologous Chromosome Pairing in a Tetraploid Intergeneric Somatic Hybrid (Citrus reticulata + Poncirus trifoliata) Revealed by Molecular Marker Inheritance

Triploid Citrus Genotypes Have a Better Tolerance to Natural Chilling Conditions of Photosynthetic Capacities and Specific Leaf Volatile Organic Compounds

Male and female inheritance patterns in tetraploid ‘Moncada’ mandarin

Contact Info

Product

Resources

About