Origin and Spread of the Dengue Virus Type 1, Genotype V in Senegal, 2015–2019

Dieng, Idrissa; Cunha, Marielton dos Passos; Diagne, Moussa Moïse; Sembène, Pape Mbacké; Zanotto, Paolo Marinho de Andrade; Faye, Ousmane; Sall, Amadou Alpha

doi:10.3390/v13010057

Cited by 33 publications

(33 citation statements)

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“…Phylogenetically, the Louga, Fatick and Thiès strains are identical and closely related (99.18% nucleotides) to a DENV strain from Singapore (KM403584.1). Based on the complete E-gene sequences, Dieng and colleagues reported that the Louga 2017 and Fatick 2018 DENV-1 isolates fall into a monophyletic cluster and were introduced into Senegal (Medina Gounass) from Singapore in 2014 (95% HPD = 2012.88–2014.84) [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, 200 µl of each sample diluted 1:10 in Leibovitz 15 (L15) medium in a 25 cm 2 flask of C6/36 cells at 80% confluence, followed by incubation at 28 °C for 1 h to allow virus binding and entry. After incubation, L15 medium containing 5% FBS, 1% penicillin and streptomycin, 0.05% fungizone was added to the flask and incubated for 8 days according to the protocol described by Dieng and colleagues [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

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Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

et al. 2021

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Background Dengue fever is a mosquito born disease associated with self-limited to life threatening illness. First detected in Senegal in the nineteenth century, and despite its growing incidence this last decade, significant knowledge gaps exist in our knowledge of genetic diversity of circulating strains. This study highlights the circulating serotypes and genotypes between January 2017 and December 2018 and their spatial and temporal distribution throughout all regions of Senegal. Methods We used 56 dengue virus (DENV) strains for the analysis collected from 11 sampling areas: 39 from all regions of Senegal, and 17 isolates from Thiès, a particular area of the country. Two real time RT-qPCR systems were used to confirm dengue infection and corresponding serotypes. For molecular characterization, CprM gene was sequenced and submitted to phylogenetic analysis for serotypes and genotypes assignment. Results Three dengue virus serotypes (DENV-1–3) were detected by all used methods. DENV-3 was detected in 50% (28/56) of the isolates, followed by DENV-1 and DENV-2, each representing 25% (14/56) of the isolates. DENV-3 belongs to genotype III, DENV-1 to genotype V and DENV-2 to Cosmopolitan genotype. Serotype 3 was detected in 7 sampling locations and a co-circulation of different serotypes was observed in Thiès, Fatick and Richard-toll. Conclusions These results emphasize the need of continuous DENV surveillance in Senegal to detect DENV cases, to define circulating serotypes/genotypes and to prevent the spread and the occurrence of severe cases.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

et al. 2021

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“…Expansion of multiple DENV serotypes in Africa may be caused by several factors. International travel of infected people from epidemic and endemic countries has been associated with the introduction of DENV-1 and DENV-3 serotypes in several African countries [83,84]. Spill-over of sylvatic DENV-2 strains from forest Aedes mosquitoes into a human transmission cycle possibly facilitates the spread into urban or new geographical areas with the potential to cause epi-demics [85,86].…”

Section: Discussionmentioning

confidence: 99%

Dengue Virus Infection and Associated Risk Factors in Africa: A Systematic Review and Meta-Analysis

Mwanyika

Mboera

Rugarabamu

et al. 2021

Viruses

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Dengue contributes a significant burden on global public health and economies. In Africa, the burden of dengue virus (DENV) infection is not well described. This review was undertaken to determine the prevalence of dengue and associated risk factors. A literature search was done on PubMed/MEDLINE, Scopus, Embase, and Google Scholar databases to identify articles published between 1960 and 2020. Meta-analysis was performed using a random-effect model at a 95% confidence interval, followed by subgroup meta-analysis to determine the overall prevalence. Between 1960 and 2020, 45 outbreaks were identified, of which 17 and 16 occurred in East and West Africa, respectively. Dengue virus serotype 1 (DENV-1) and DENV-2 were the dominant serotypes contributing to 60% of the epidemics. Of 2211 cases reported between 2009 and 2020; 1954 (88.4%) were reported during outbreaks. Overall, the prevalence of dengue was 29% (95% CI: 20–39%) and 3% (95% CI: 1–5%) during the outbreak and non-outbreak periods, respectively. Old age (6/21 studies), lack of mosquito control (6/21), urban residence (4/21), climate change (3/21), and recent history of travel (3/21) were the leading risk factors. This review reports a high burden of dengue and increased risk of severe disease in Africa. Our findings provide useful information for clinical practice and health policy decisions to implement effective interventions.

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“…West Africa has experienced several outbreaks of DENV and it is the main MBV circulating in the human population and posed a major public health threat. It is likely that the first detection of DENV in West Africa was in Ibadan, Nigeria in 1964 from febrile patients [ 42 ]. However, the most recent outbreak of DENV was reported in Ouagadougou, Burkina Faso in 2017 [ 59 ].…”

Section: Discovery Of Mosquito-associated Virusesmentioning

confidence: 99%

Mosquito-Associated Viruses and Their Related Mosquitoes in West Africa

Agboli

Zahouli

Badolo

et al. 2021

Viruses

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Mosquito-associated viruses (MAVs), including mosquito-specific viruses (MSVs) and mosquito-borne (arbo)viruses (MBVs), are an increasing public, veterinary, and global health concern, and West Africa is projected to be the next front for arboviral diseases. As in-depth knowledge of the ecologies of both western African MAVs and related mosquitoes is still limited, we review available and comprehensive data on their diversity, abundance, and distribution. Data on MAVs’ occurrence and related mosquitoes were extracted from peer-reviewed publications. Data on MSVs, and mosquito and vertebrate host ranges are sparse. However, more data are available on MBVs (i.e., dengue, yellow fever, chikungunya, Zika, and Rift Valley fever viruses), detected in wild and domestic animals, and humans, with infections more concentrated in urban areas and areas affected by strong anthropogenic changes. Aedes aegypti, Culex quinquefasciatus, and Aedes albopictus are incriminated as key arbovirus vectors. These findings outline MAV, related mosquitoes, key knowledge gaps, and future research areas. Additionally, these data highlight the need to increase our understanding of MAVs and their impact on host mosquito ecology, to improve our knowledge of arbovirus transmission, and to develop specific strategies and capacities for arboviral disease surveillance, diagnostic, prevention, control, and outbreak responses in West Africa.

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Origin and Spread of the Dengue Virus Type 1, Genotype V in Senegal, 2015–2019

Cited by 33 publications

References 45 publications

Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

Dengue Virus Infection and Associated Risk Factors in Africa: A Systematic Review and Meta-Analysis

Mosquito-Associated Viruses and Their Related Mosquitoes in West Africa

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