Origin and Early Development of Secondary Embryos in Quercus suber L.

Puigderrajols, Pere; Fernández-Guijarro, B.; Toribio, M.; Molinas, Marisa

doi:10.1086/297389

Cited by 37 publications

(29 citation statements)

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“…In a PGR-free medium, the embryonic axis shows a high capacity for the formation of secondary embryos (Puigderrajols et al 1996); therefore, in this tissue, the process depends on endogenous regulation. However, to yield a morphogenic response, cotyledons need an exogenous induction.…”

Section: Discussionmentioning

confidence: 99%

“…There are other examples in which the internal origin of somatic embryos is reported: i.e., Solanum aviculare, in which embryogenesis is related to parenchyma cells associated with vascular traces close to the cut ends (Alizadeh and Mantell 1991). This origin contrasts with that found in the axis, in which the outer layers of the root cap gave rise to secondary embryos (Puigderrajols et al 1996). This observation demonstrates that specific cells in different explants may be committed to providing the embryogenic response, at least that of multicellular origin.…”

Section: Discussionmentioning

confidence: 99%

“…1 cm in length), or excised cotyledons were used as starting explants in the different experiments. For a more detailed description, see Puigderrajols et al (1996).…”

Section: Methodsmentioning

confidence: 99%

“…For light microscopy, samples were fixed in 10% buffered formalin (pH 7.2), dehydrated through an isopropyl alcohol series, and embedded in glycol-methacrylate. The sections were stained with the periodic acid-Schiff reaction, toluidine blue, or thionin (Puigderrajols et al 1996). Fluorescence was observed using a Vanox epifluorescence microscope equipped with a HBO200 mercury burner.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous paper on cork oak recurrent somatic embryogenesis (Puigderrajols et al 1996), we reported that on PGRfree medium, secondary embryos arose from a slight initial proliferation at the external layers of the root cap and developed mainly following a multicellular budding pattern. Under these culture conditions, we seldom observed secondary embryos originating from cotyledons.…”

Section: Introductionmentioning

confidence: 94%

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Histology of Organogenic and Embryogenic Responses in Cotyledons of Somatic Embryos of Quercus suber L.

Puigderrajols

Celestino

Suils

et al. 2000

International Journal of Plant Sciences

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. In cork oak (Quercus suber L.), recurrent embryogenesis is produced in vitro through autoembryony without exogenous plant growth regulators (PGRs); secondary embryos appear on the embryo axis but seldom on cotyledons. Focusing mainly on the histological origin of neoformations, we investigated the influence of the embryo axis and exogenous PGRs on the embryogenic potential of somatic embryo cotyledons. Isolated cotyledons of somatic embryos became necrotic when cultured on PGR-free medium but gave secondary embryos when cultured on media containing benzyladenine and naphthaleneacetic acid. Cotyledons of cork oak somatic embryos are competent to give embryogenic responses. Isolated cotyledons without a petiole showed a lower percentage of embryogenic response than did those with a petiole. In petioles, somatic embryos arose from inner parenchyma tissues following a multicellular budding pattern. Joined to the embryo axis, cotyledons did not show morphogenic responses when cultured on PGR-free medium but revealed budlike and phylloid formations when cultured on medium with PGRs. The different morphogenic behavior displayed by somatic cotyledons indicates an influence of the embryo axis and indicates a relationship between organogenic and embryogenic regeneration pathways. HISTOLOGY OF ORGANOGENIC AND EMBRYOGENIC RESPONSES IN COTYLEDONS OF SOMATIC EMBRYOS OF QUERCUS SUBER L.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…1 cm in length), or excised cotyledons were used as starting explants in the different experiments. For a more detailed description, see Puigderrajols et al (1996).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

See 3 more Smart Citations