Oriented Protein Nanoarrays on Block Copolymer Template

Shen, Lei; Zhu, Jintao

doi:10.1002/marc.201500687

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…In another example, PEO block incorporating a maleimide group were used in polystyrene-block-poly(ethylene oxide) (PS-b-PEO) diblock copolymers to nanopattern small peptides through the maleimide-cystein linkage [52]. Attempts to extend the versatility of this approach have also been reported, as the binding of poly-histidine tagged proteins on polystyrene-block-poly(2hydroxyethyl methacrylate) (PS-b-PHEMA) diblock copolymers mixed with iminodiacetic-terminated PS [60]. Although the opportunities to tailor well-studied block copolymer systems exponentially increases by customizing their synthesis, the consequences on the self-assembled nanostructure are not negligible [12,59].…”

Section: Discussionmentioning

confidence: 99%

Large-Area Biomolecule Nanopatterns on Diblock Copolymer Surfaces for Cell Adhesion Studies

et al. 2019

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Cell membrane receptors bind to extracellular ligands, triggering intracellular signal transduction pathways that result in specific cell function. Some receptors require to be associated forming clusters for effective signaling. Increasing evidences suggest that receptor clustering is subjected to spatially controlled ligand distribution at the nanoscale. Herein we present a method to produce in an easy, straightforward process, nanopatterns of biomolecular ligands to study ligand–receptor processes involving multivalent interactions. We based our platform in self-assembled diblock copolymers composed of poly(styrene) (PS) and poly(methyl methacrylate) (PMMA) that form PMMA nanodomains in a closed-packed hexagonal arrangement. Upon PMMA selective functionalization, biomolecular nanopatterns over large areas are produced. Nanopattern size and spacing can be controlled by the composition of the block-copolymer selected. Nanopatterns of cell adhesive peptides of different size and spacing were produced, and their impact in integrin receptor clustering and the formation of cell focal adhesions was studied. Cells on ligand nanopatterns showed an increased number of focal contacts, which were, in turn, more matured than those found in cells cultured on randomly presenting ligands. These findings suggest that our methodology is a suitable, versatile tool to study and control receptor clustering signaling and downstream cell behavior through a surface-based ligand patterning technique.

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Section: Discussionmentioning

confidence: 99%

Large-Area Biomolecule Nanopatterns on Diblock Copolymer Surfaces for Cell Adhesion Studies

et al. 2019

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“…For instance, a mixture of PS-b-PHEMA and PS end-functionalized with iminodiacetic acid groups (IDA) results in the assembly of nanopatterns where the PS domains bear IDA and can be used for further immobilization of histidine-tagged protein (Figure 11). [56] In a different strategy, Spatz et al have proposed to use BCP nanopatterns as a sacrificing template for printing peptides. [57] The advantage of this approach is that the space between individual peptides can be precisely controlled.…”

Section: Bcp Nanopatterns As Templates For Proteins Adsorptionmentioning

confidence: 99%

Preparation, Properties, and Bioapplications of Block Copolymer Nanopatterns

Fontelo,

Reis,

Novoa‐Carballal

et al. 2023

Adv Healthcare Materials

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Block copolymer (BCP) self‐assembly has emerged as a feasible method for large‐scale fabrication with remarkable precision – features that are not common for most of the nanofabrication techniques. In this review, we present recent advancements in the molecular design of BCP along with state‐of‐the‐art processing methodologies based on microphase separation alone or its combination with different lithography methods. Furthermore, we explore the bioapplications of the generated nanopatterns in the development of protein arrays, cell‐selective surfaces, and antibacterial coatings. Finally, we outline the current challenges in the field and discuss the potential breakthroughs that can be achieved by adopting BCP approaches already applied in the fabrication electronic devices.This article is protected by copyright. All rights reserved

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“…The most common morphologies are a cubic ordering of spheres, hexagonally ordered cylinders, and lamellae. , The fabrication of BCP thin films adds even more possible morphologies due to the influence of interface-directed ordering. − The variety of accessible, tunable self-assembled nanostructures with a long-range order makes BCP thin films highly attractive as functional materials . Films of BCPs can be used to order protein on top of − or inside thin films. − Furthermore, the Olsen group demonstrated the self-assembly of proteins as part of protein–polymer diblock copolymers in three-dimensional solid-state nanostructures. − Another interesting approach was presented by Rao et al who arranged gold nanoparticles through self-assembled peptide-functionalized BCPs. For the organization of proteins within BCP films, however, particular challenges arise.…”

Section: Introductionmentioning

confidence: 99%

Polystyrene-block-poly(ethylene oxide) Thin Films Fabricated from a Solvent Mixture for the Co-Assembly of Polymers and Proteins

Kollmetz

Gerrard

et al. 2020

ACS Omega

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The co-assembly of peptides and proteins in poly(styrene- block -ethylene oxide) (PS- b -PEO) thin films has proven to be a promising method to fabricate polymer–biomolecule functional materials. Contrary to the covalent immobilization of biomolecules on surfaces, co-assembly presents the opportunity to arrange cargo within thin films, which can be released upon exposure to an aqueous environment. The use of a mixed solvent system ensures the solubilization of hydrophobic polymer as well as the solubilization and protection of the biomolecule cargo. However, to produce largely defect-free films of PS- b -PEO from a solvent mixture containing water is challenging due to the narrow range of solvent miscibility and polymer/protein solubility. This work explores the limits of using a benzene/methanol/water solvent mixture for the production of thin PS- b -PEO films and provides a template for the fabrication optimization of block copolymer thin films in different complex solvent systems. The film quality is analyzed using optical microscopy and atomic force microscopy and correlated to the solvent composition. By adjusting the solvent composition to 80/18.8/1.2 vol % benzene/methanol/water, it was possible to reliably fabricate thin films with less than 1% macroscopic defect surface coverage. Using the optimized solvent composition, we also demonstrate the fabrication of ordered PS- b -PEO films containing lysozyme. Furthermore, we show the release of lysozyme into aqueous media, which highlights the potential use of such films for drug delivery applications.

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Oriented Protein Nanoarrays on Block Copolymer Template

Cited by 9 publications

References 47 publications

Large-Area Biomolecule Nanopatterns on Diblock Copolymer Surfaces for Cell Adhesion Studies

Large-Area Biomolecule Nanopatterns on Diblock Copolymer Surfaces for Cell Adhesion Studies

Preparation, Properties, and Bioapplications of Block Copolymer Nanopatterns

Polystyrene-block-poly(ethylene oxide) Thin Films Fabricated from a Solvent Mixture for the Co-Assembly of Polymers and Proteins

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